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TGFBI Protein, Human(HEK293, His)

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Based on 1 publication(s) in Google Scholar

TGFBI Protein, Human(HEK293, His) is the recombinant human-derived TGFBI, expressed by HEK293 , with C-10*His labeled tag. ,

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for TGFBI Protein, Human(HEK293, His)
  • TGFBI Protein, Human(HEK293, His) Product Image
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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
Top Publications Citing

Use of MCE Products

Biological Activity

Description

TGFBI Protein, Human(HEK293, His) is the recombinant human-derived TGFBI, expressed by HEK293 , with C-10*His labeled tag. ,

Background

TGFBI, a multifaceted protein, assumes a pivotal role in cell adhesion, with implications for diverse cellular processes. Its involvement in cell-collagen interactions further underscores its significance in mediating cellular responses and adhesion-related events. Notably, TGFBI exhibits a binding affinity for a spectrum of collagens, including type I, II, and IV, implicating its participation in various extracellular matrix interactions and highlighting its potential impact on cellular adhesion to different collagen types.

Verified Bioactivity

1.Measured by its ability to induce adhesion of ATDC5 mouse chondrogenic cells. When 5 x 104 cells/well are added to Recombinant rh beta IG-H3 coated plates (5 µg/mL with 100 µL/well), greater than 45.00%-68.78% cells will adhere.
2.Measured by its ability to induce adhesion of ATDC5 mouse chondrogenic cells. When 5 x 104 cells/well are added to Recombinant Human TGFBI coated plates (5 µg/mL with 100 µL/well), 66.00%-77.08% cells will adhere after 1 hour incubation.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 90%, as determined by reducing SDS-PAGE.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag C-10*His
  • Accession

    Q15582/NP_000349.1 (G24-H683)

  • Gene ID
    7045
  • Molecular Construction
    • N-term
    • TGFBI (G24-H683)
      Accession # Q15582/NP_000349.1
    • 10*His
    • C-term
  • Protein Length

    Full Length of Mature Protein

  • Synonyms

    TGFBI; RGD-Containing Collagen-Associated Protein; Prev. CSD1; Beta Ig-H3; Prev. CSD2; RGD-CAP; Prev. CSD3; Transforming Growth Factor, Beta-Induced, 68kDa; Prev. LCD1; Transforming Growth Factor Beta-Induced 68kDa; BIGH3; Transforming Growth Factor Beta;

  • AA Sequence

    GPAKSPYQLVLQHSRLRGRQHGPNVCAVQKVIGTNRKYFTNCKQWYQRKICGKSTVISYECCPGYEKVPGEKGCPAALPLSNLYETLGVVGSTTTQLYTDRTEKLRPEMEGPGSFTIFAPSNEAWASLPAEVLDSLVSNVNIELLNALRYHMVGRRVLTDELKHGMTLTSMYQNSNIQIHHYPNGIVTVNCARLLKADHHATNGVVHLIDKVISTITNNIQQIIEIEDTFETLRAAVAASGLNTMLEGNGQYTLLAPTNEAFEKIPSETLNRILGDPEALRDLLNNHILKSAMCAEAIVAGLSVETLEGTTLEVGCSGDMLTINGKAIISNKDILATNGVIHYIDELLIPDSAKTLFELAAESDVSTAIDLFRQAGLGNHLSGSERLTLLAPLNSVFKDGTPPIDAHTRNLLRNHIIKDQLASKYLYHGQTLETLGGKKLRVFVYRNSLCIENSCIAAHDKRGRYGTLFTMDRVLTPPMGTVMDVLKGDNRFSMLVAAIQSAGLTETLNREGVYTVFAPTNEAFRALPPRERSRLLGDAKELANILKYHIGDEILVSGGIGALVRLKSLQGDKLEVSLKNNVVSVNKEPVAEPDIMATNGVVHVITNVLQPPANRPQERGDELADSALEIFKQASAFSRASQRSVRLAPVYQKLLERMKH

  • Predicted Molecular Mass

    72.4 kDa

  • Molecular Weight

    Approximately 75 kDa, based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 90%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder

Formulation

1.Lyophilized from a 0.22 μm filtered solution of PBS, pH 8.0.
2.Lyophilized from a 0.22 μm filtered solution of 10 mM Sodium Citrate, pH 6.0.
Please refer to the lot-specific COA for specific buffer information.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 90%, as determined by reducing SDS-PAGE.

  • TGFBI Protein, Human(HEK293, His) Product Image
    1/16
  • Experimental Validation Results for TGFBI Protein, Human(HEK293, His)
  • TGFBI Protein, Human(HEK293, His) Product Image