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CHI3L1 Protein, Human (HEK293, His)

4 Cited Publications
Customer Review

Based on 4 publication(s) in Google Scholar

The CHI3L1 protein is a binding lectin that lacks chitinase activity. CHI3L1 contributes to tissue remodeling, cellular adaptation to environmental changes, and T helper type 2 inflammatory responses. CHI3L1 also controls hyperoxia-induced injury, inflammation, and epithelial cell apoptosis. CHI3L1 Protein, Human (HEK293, His) is the recombinant human-derived CHI3L1 protein, expressed by HEK293 , with C-6*His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for CHI3L1 Protein, Human (HEK293, His)
  • Experimental Validation Results for CHI3L1 Protein, Human (HEK293, His)
  • Experimental Validation Results for CHI3L1 Protein, Human (HEK293, His)
  • Experimental Validation Results for CHI3L1 Protein, Human (HEK293, His)
  • Experimental Validation Results for CHI3L1 Protein, Human (HEK293, His)
  • Experimental Validation Results for CHI3L1 Protein, Human (HEK293, His)
  • Experimental Validation Results for CHI3L1 Protein, Human (HEK293, His)
  • CHI3L1 Protein, Human (HEK293, His) Product Image
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  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
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Biological Activity

Description

The CHI3L1 protein is a binding lectin that lacks chitinase activity. CHI3L1 contributes to tissue remodeling, cellular adaptation to environmental changes, and T helper type 2 inflammatory responses. CHI3L1 also controls hyperoxia-induced injury, inflammation, and epithelial cell apoptosis. CHI3L1 Protein, Human (HEK293, His) is the recombinant human-derived CHI3L1 protein, expressed by HEK293 , with C-6*His labeled tag.

Background

Chitinase-3-like protein 1 (CHI3L1) is a carbohydrate-binding lectin with a specific affinity for chitin, although it lacks chitinase activity. Its main role extends beyond chitin degradation, as it is implicated in tissue remodeling and cellular responses to environmental changes. CHI3L1 plays a crucial role in T-helper cell type 2 (Th2) inflammatory responses and IL-13-induced inflammation, influencing allergen sensitization, inflammatory cell apoptosis, dendritic cell accumulation, and the differentiation of M2 macrophages. Additionally, it facilitates the invasion of pathogenic enteric bacteria into colonic mucosa and lymphoid organs. CHI3L1 is involved in the activation of the AKT1 signaling pathway, leading to IL8 production in colonic epithelial cells. Furthermore, it regulates antibacterial responses in the lungs, contributing to macrophage bacterial killing, controlling bacterial dissemination, and enhancing host tolerance. In lung tissues, CHI3L1 also plays a role in regulating hyperoxia-induced injury, inflammation, and epithelial apoptosis. Structurally, CHI3L1 functions as a monomer.

Verified Bioactivity

Measured by the ability of the immobilized protein to support the adhesion of FaDu human squamous cell carcinoma cells. The ED50 for this effect is 0.6-3.0 μg/mL.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

  • Bioactivity - Cell-Based Assay

    Bioactivity - Cell-Based Assay

    Measured by the ability of the immobilized protein to support the adhesion of FaDu human squamous cell carcinoma cells. The ED50 for this effect is 2.603 μg/mL, corresponding to a specific activity is 384.1721 units/mg.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag C-6*His
  • Accession

    P36222 (Y22-T383)

  • Gene ID
    1116  [NCBI]
  • Molecular Construction
    • N-term
    • CHI3L1 (Y22-T383)
      Accession # P36222
    • 6*His
    • C-term
  • Protein Length

    Full Length of Mature Protein

  • Synonyms

    CHI3L1; HCGP-39; Chitinase 3 Like 1; CGP-39; Chitinase-3-Like Protein 1; YKL-40; YKL40; GP-39; YK-40; CHI3L1 Protein; GP39; HC-Gp39; Chitinase 3-Like 1 (Cartilage Glycoprotein-39); HCGP-3P; Cartilage Glycoprotein-39; YYL-40; Cartilage Glycoprotein 39; ASR

  • AA Sequence

    YKLVCYYTSWSQYREGDGSCFPDALDRFLCTHIIYSFANISNDHIDTWEWNDVTLYGMLNTLKNRNPNLKTLLSVGGWNFGSQRFSKIASNTQSRRTFIKSVPPFLRTHGFDGLDLAWLYPGRRDKQHFTTLIKEMKAEFIKEAQPGKKQLLLSAALSAGKVTIDSSYDIAKISQHLDFISIMTYDFHGAWRGTTGHHSPLFRGQEDASPDRFSNTDYAVGYMLRLGAPASKLVMGIPTFGRSFTLASSETGVGAPISGPGIPGRFTKEAGTLAYYEICDFLRGATVHRILGQQVPYATKGNQWVGYDDQESVKSKVQYLKDRQLAGAMVWALDLDDFQGSFCGQDLRFPLTNAIKDALAAT

  • Predicted Molecular Mass

    41.3 kDa

  • Molecular Weight

    Approximately 43-46 kDa, based on SDS-PAGE under reducing conditions.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder

Formulation

1.Lyophilized from a 0.22 μm filtered solution of 20 mM Tris-HCl, 300 mM NaCl, 2 mM EDTA, pH 8.5.
2.Lyophilized from a 0.22 μm filtered solution of 20 mM PB, 150 mM NaCl, 2 mM EDTA, pH 7.4.
3.Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.
Please refer to the lot-specific COA for specific buffer information.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured by the ability of the immobilized protein to support the adhesion of FaDu human squamous cell carcinoma cells. The ED50 for this effect is 2.603 μg/mL, corresponding to a specific activity is 384.1721 units/mg.

  • 1/16

    CHI3L1 Protein, Human (HEK293, His) purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Feb;12(6):e2310169. [Abstract]

    Customer Validation
     Representative images and quantification of transwell assay at 24 h after stimulation with different concentrations of CHI3L1 Protein (0-200 ng/mL). n = 6/ea.

  • 1/16

    CHI3L1 Protein, Human (HEK293, His) purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Feb;12(6):e2310169. [Abstract]

    Customer Validation
     Representative images and relative quantification of collagen gel contractility assay after 24 h of CHI3L1 Protein (0-200 ng/mL) administration.

  • 1/16

    CHI3L1 Protein, Human (HEK293, His) purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Feb;12(6):e2310169. [Abstract]

    Customer Validation
     CO-IP analysis of CHI3L1 Protein and rIL-17RA in a cell-free system.

  • 1/16

    CHI3L1 Protein, Human (HEK293, His) purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Feb;12(6):e2310169. [Abstract]

    Customer Validation
     Representative immunofluorescent staining images with Ki67 at 24 h after CHI3L1 Protein treatment.

  • 1/16

    CHI3L1 Protein, Human (HEK293, His) purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Feb;12(6):e2310169. [Abstract]

    Customer Validation
     Representative images and quantification of transwell assay at 24 h with CHI3L1 Protein stimulation.

  • CHI3L1 Protein, Human (HEK293, His) Product Image
    1/16
  • Experimental Validation Results for CHI3L1 Protein, Human (HEK293, His)
  • Experimental Validation Results for CHI3L1 Protein, Human (HEK293, His)
  • Experimental Validation Results for CHI3L1 Protein, Human (HEK293, His)
  • Experimental Validation Results for CHI3L1 Protein, Human (HEK293, His)
  • Experimental Validation Results for CHI3L1 Protein, Human (HEK293, His)
  • Experimental Validation Results for CHI3L1 Protein, Human (HEK293, His)
  • Experimental Validation Results for CHI3L1 Protein, Human (HEK293, His)
  • CHI3L1 Protein, Human (HEK293, His) Product Image