Copy Link Print Information

COMP Protein, Human (HEK293, His)

2 Cited Publications
Customer Review

Based on 2 publication(s) in Google Scholar

The COMP protein is critical for cartilage integrity and interacts with extracellular matrix proteins to promote the connection of chondrocytes to the cartilage matrix through integrin receptors. Its role in the pathogenesis of osteoarthritis emphasizes the significance of joint health. COMP Protein, Human (HEK293, His) is the recombinant human-derived COMP protein, expressed by HEK293 , with C-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for COMP Protein, Human (HEK293, His)
  • COMP Protein, Human (HEK293, His) Product Image
Size
Price
Stock
Quantity
Amount: USD 0.00
Please select quantity
or
or

* Please select Quantity before adding items.

To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download

To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
Top Publications Citing

Use of MCE Products

Biological Activity

Description

The COMP protein is critical for cartilage integrity and interacts with extracellular matrix proteins to promote the connection of chondrocytes to the cartilage matrix through integrin receptors. Its role in the pathogenesis of osteoarthritis emphasizes the significance of joint health. COMP Protein, Human (HEK293, His) is the recombinant human-derived COMP protein, expressed by HEK293 , with C-His labeled tag.

Background

The COMP protein assumes a crucial role in preserving the structural integrity of cartilage by interacting with other extracellular matrix proteins, including collagens and fibronectin. It facilitates the connection between chondrocytes and the cartilage extracellular matrix by engaging with cell surface integrin receptors. Additionally, COMP's involvement in the pathogenesis of osteoarthritis underscores its potential significance in joint health. Beyond its structural role, COMP emerges as a potent suppressor of apoptosis in both primary chondrocytes and transformed cells. Its anti-apoptotic effects are mediated by inhibiting caspase-3 activation and inducing the IAP family of survival proteins (BIRC3, BIRC2, BIRC5, and XIAP). Furthermore, COMP plays an essential role in maintaining the contractile and differentiated phenotype of vascular smooth muscle cells under both physiological and pathological stimuli, achieved through its interaction with ITGA7. This multifaceted functionality positions COMP as a key player in the maintenance of cartilage structure, joint health, and cellular survival processes.

Verified Bioactivity

1. Measured by its ability to induce adhesion of ATDC5 mouse chondrogenic cells.When cells are added to COMP-coated plates (0.625 μg/mL and 100 μL/well), approximately ≥30% cells will adhere specifically after 60 minutes at 37°C.
2. Measured by the ability of the immobilized protein to support the adhesion of ATDC5 cells. When 5 x 104 cells/well are added to Recombinant Human COMP coated plates (0.625µg/mL, 100 µL/well), 48-60% cells will adhere after 1 hour incubation.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 90%, as determined by reducing SDS-PAGE.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag C-His
  • Accession

    P49747-1 (Q21-A757)

  • Gene ID
    1311  [NCBI]
  • Molecular Construction
    • N-term
    • COMP (Q21-A757)
      Accession # P49747-1
    • His
    • C-term
  • Protein Length

    Full Length of Isoform-1

  • Synonyms

    COMP; TSP-5; Prev. PSACH; Cartilage Oligomeric Matrix Protein (Pseudoachondroplasia, Epiphyseal Dysplasia 1, Multiple); Prev. EDM1; Multiple Epiphyseal Dysplasia; Prev. EPD1; Pseudoachondroplasia (Epiphyseal Dysplasia 1, Multiple); TSP5; Alternative Prote

  • AA Sequence

    QGQSPLGSDLGPQMLRELQETNAALQDVRELLRQQVREITFLKNTVMECDACGMQQSVRTGLPSVRPLLHCAPGFCFPGVACIQTESGARCGPCPAGFTGNGSHCTDVNECNAHPCFPRVRCINTSPGFRCEACPPGYSGPTHQGVGLAFAKANKQVCTDINECETGQHNCVPNSVCINTRGSFQCGPCQPGFVGDQASGCQRRAQRFCPDGSPSECHEHADCVLERDGSRSCVCAVGWAGNGILCGRDTDLDGFPDEKLRCPERQCRKDNCVTVPNSGQEDVDRDGIGDACDPDADGDGVPNEKDNCPLVRNPDQRNTDEDKWGDACDNCRSQKNDDQKDTDQDGRGDACDDDIDGDRIRNQADNCPRVPNSDQKDSDGDGIGDACDNCPQKSNPDQADVDHDFVGDACDSDQDQDGDGHQDSRDNCPTVPNSAQEDSDHDGQGDACDDDDDNDGVPDSRDNCRLVPNPGQEDADRDGVGDVCQDDFDADKVVDKIDVCPENAEVTLTDFRAFQTVVLDPEGDAQIDPNWVVLNQGREIVQTMNSDPGLAVGYTAFNGVDFEGTFHVNTVTDDDYAGFIFGYQDSSSFYVVMWKQMEQTYWQANPFRAVAEPGIQLKAVKSSTGPGEQLRNALWHTGDTESQVRLLWKDPRNVGWKDKKSYRWFLQHRPQVGYIRVRFYEGPELVADSNVVLDTTMRGGRLGVFCFSQENIIWANLRYRCNDTIPEDYETHQLRQA

  • Molecular Weight

    Approximately 120-130 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Glycosylation

    Yes

  • Purity

    ≥ 90%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder

Formulation

1.Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 5% trehalose, 5% mannitol and 0.01% Tween 80.
2.Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.
Please refer to the lot-specific COA for specific buffer information.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) Volume (to add to vial)
=
Mass (in vial) Mass (in vial)
÷
Desired Reconstitution Concentration Desired Reconstitution Concentration
Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

Concentration (start) Concentration (start)
×
Volume (start) Volume (start)
=
Concentration (final) Concentration (final)
×
Volume (final) Volume (final)
The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

Get Quote In-stock

Other size
Get Quote
Please select quantity
Amount: USD 0.00

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 90%, as determined by reducing SDS-PAGE.

  • COMP Protein, Human (HEK293, His) Product Image
    1/16
  • Experimental Validation Results for COMP Protein, Human (HEK293, His)
  • COMP Protein, Human (HEK293, His) Product Image