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CFHR1 Protein, Human (HEK293, His)

Customer Review

Based on 1 Customer Validation

CFHR1, a key complement regulator, actively engages in the finely tuned complement system. Dimerized CFHR1 demonstrates avidity for tissue-bound complement fragments, challenging CFH. It also associates with lipoproteins, suggesting involvement in lipid metabolism. With a unique head-to-tail homodimeric structure, CFHR1 forms heterodimers with CFHR2 or CFHR5, showcasing its versatile role in complement-related processes. CFHR1 Protein, Human (HEK293, His) is the recombinant human-derived CFHR1 protein, expressed by HEK293 , with C-6*His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for CFHR1 Protein, Human (HEK293, His)
  • Experimental Validation Results for CFHR1 Protein, Human (HEK293, His)
  • CFHR1 Protein, Human (HEK293, His) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
Top Publications Citing

Use of MCE Products

Biological Activity

Description

CFHR1, a key complement regulator, actively engages in the finely tuned complement system. Dimerized CFHR1 demonstrates avidity for tissue-bound complement fragments, challenging CFH. It also associates with lipoproteins, suggesting involvement in lipid metabolism. With a unique head-to-tail homodimeric structure, CFHR1 forms heterodimers with CFHR2 or CFHR5, showcasing its versatile role in complement-related processes. CFHR1 Protein, Human (HEK293, His) is the recombinant human-derived CFHR1 protein, expressed by HEK293 , with C-6*His labeled tag.

Background

CFHR1 emerges as a pivotal figure in the intricate realm of complement regulation, showcasing its active involvement in this finely tuned system. In its dimerized forms, CFHR1 exhibits a remarkable avidity for tissue-bound complement fragments, effectively challenging the physiological complement inhibitor CFH. Beyond its regulatory prowess, CFHR1 displays an affinity for association with lipoproteins, hinting at a potential role in lipid metabolism. The unique structural arrangement of CFHR1 includes a head-to-tail homodimeric configuration, and it further forms heterodimers with CFHR2 or CFHR5, emphasizing its versatile engagement in the multifaceted orchestration of complement-related processes.

Verified Bioactivity

Immobilized Human CFHR1 at 1 μg/mL (100 μL/well) can bind Anti-CFHR1. The ED50 for this effect is 183.9 ng/mL.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

  • Bioactivity - ELISA

    Bioactivity - ELISA

    Immobilized Human CFHR1 at 1 μg/mL (100 μL/well) can bind Anti-CFHR1. The ED50 for this effect is 183.9 ng/mL.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag C-6*His
  • Accession

    Q03591 (E19-R330)

  • Gene ID
    3078  [NCBI]
  • Molecular Construction
    • N-term
    • CFHR1 (E19-R330)
      Accession # Q03591
    • 6*His
    • C-term
  • Protein Length

    Full Length of Mature Protein

  • Synonyms

    CFHR1; Complement Factor H-Related 1 Pseudogene; Prev. CFHL1P; H Factor (Complement)-Like 1; Prev. CFHR1P; H Factor (Complement)-Like 2; Prev. CFHL1; H Factor-Like Protein 1; Prev. HFL1; H-Factor-Like 1; Prev. HFL2; FHL-1; FHR1; FHR-1; CFHL; H36; H36-1; C

  • AA Sequence

    EATFCDFPKINHGILYDEEKYKPFSQVPTGEVFYYSCEYNFVSPSKSFWTRITCTEEGWSPTPKCLRLCFFPFVENGHSESSGQTHLEGDTVQIICNTGYRLQNNENNISCVERGWSTPPKCRSTDTSCVNPPTVQNAHILSRQMSKYPSGERVRYECRSPYEMFGDEEVMCLNGNWTEPPQCKDSTGKCGPPPPIDNGDITSFPLSVYAPASSVEYQCQNLYQLEGNKRITCRNGQWSEPPKCLHPCVISREIMENYNIALRWTAKQKLYLRTGESAEFVCKRGYRLSSRSHTLRTTCWDGKLEYPTCAKR

  • Molecular Weight

    Approximately 37-52 kDa, based on SDS-PAGE under reducing conditions.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder

Formulation

1.Lyophilized from a 0.22 μm filtered solution of 20 mM PB, 8% trehalose, 4% mannitol, 50 mM NaCl, 0.05% Tween 80, pH 7.5.
2.Lyophilized from a 0.22 μm filtered solution of PBS, pH 8.0, 2 mM EDTA.
Please refer to the lot-specific COA for specific buffer information.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Immobilized Human CFHR1 at 1 μg/mL (100 μL/well) can bind Anti-CFHR1. The ED50 for this effect is 183.9 ng/mL.

  • CFHR1 Protein, Human (HEK293, His) Product Image
    1/16
  • Experimental Validation Results for CFHR1 Protein, Human (HEK293, His)
  • Experimental Validation Results for CFHR1 Protein, Human (HEK293, His)
  • CFHR1 Protein, Human (HEK293, His) Product Image