MGP Protein, Human (His)
Based on 1 Customer Validation
MGP Protein, Human (His) is the recombinant human-derived MGP, expressed by E. coli , with C-6*His labeled tag. ,
- Species: Human
- Source: E. coli
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
MGP Protein, Human (His) is the recombinant human-derived MGP, expressed by E. coli , with C-6*His labeled tag. ,
Background
MGP is a protein that associates with the organic matrix of bone and cartilage and is thought to act as an inhibitor of bone formation.
Verified Bioactivity
1.Measured in a cell proliferation assay using HT-29 cells. The ED50 for this effect is 61.07 ng/mL, corresponding to a specific activity is 1.637×104 Unit/mg.
2.Recombinant Human Vitronectin immobilized on CM5 Chip can bind Human MGP with an affinity constant of 0.1-1.0 μM as determined in a SPR assay.
MCE Validation Data
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Purity - SDS-PAGE
Purity - SDS-PAGE
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Bioactivity - Cell-Based Assay
Bioactivity - Cell-Based Assay
Technical Parameters
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Species Human
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Source E. coli
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Tag C-6*His
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Accession
P08493-1 (Y20-F96)
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Gene ID4256
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Protein Length
Full Length of Isoform-1
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Synonyms
MGP; MGLAP; Matrix Gla Protein; GIG36; Cell Growth-Inhibiting Gene 36 Protein; NTI
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AA Sequence
YESHESMESYELNPFINRRNANTFISPQQRWRAKVQERIRERSKPVHELNREACDDYRLCERYAMVYGYNAAYNRYF
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Molecular Weight
Approximately 13 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder
1.Lyophilized from a 0.22 μm filtered solution of PBS, 6-8% trehalose, pH 7.4.
2.Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
Please refer to the lot-specific COA for specific buffer information.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (235 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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