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GAS6 Protein, Human (HEK293, His)

Customer Review

Based on 1 Customer Validation

The GAS6 protein is a ligand for AXL, TYRO3 and MER receptors and affects a variety of cellular processes.GAS6/AXL signaling promotes endothelial cell survival, aids cytokine signaling in natural killer cell development, supports liver regeneration, affects gonadotropin-releasing hormone neuron survival and migration, modulates platelet activation, and regulates thrombotic responses.GAS6 Protein, Human (HEK293, His) is the recombinant human-derived GAS6 protein, expressed by HEK293 , with C-6*His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for GAS6 Protein, Human (HEK293, His)
  • Experimental Validation Results for GAS6 Protein, Human (HEK293, His)
  • GAS6 Protein, Human (HEK293, His) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

The GAS6 protein is a ligand for AXL, TYRO3 and MER receptors and affects a variety of cellular processes.GAS6/AXL signaling promotes endothelial cell survival, aids cytokine signaling in natural killer cell development, supports liver regeneration, affects gonadotropin-releasing hormone neuron survival and migration, modulates platelet activation, and regulates thrombotic responses.GAS6 Protein, Human (HEK293, His) is the recombinant human-derived GAS6 protein, expressed by HEK293 , with C-6*His labeled tag.

Background

GAS6, a ligand for tyrosine-protein kinase receptors AXL, TYRO3, and MER, is implicated in diverse cellular processes including cell growth, survival, adhesion, and migration. GAS6/AXL signaling contributes to endothelial cell survival under acidic conditions by preventing apoptosis, facilitates optimal cytokine signaling during human natural killer cell development, plays a role in hepatic regeneration, influences gonadotropin-releasing hormone neuron survival and migration, regulates platelet activation, and modulates thrombotic responses. Additionally, in microbial infections, GAS6 can act as a bridge between virus envelope phosphatidylserine and the TAM receptor tyrosine kinase Axl, facilitating viral entry through apoptotic mimicry. This unique function extends to its involvement in the entry processes of diverse viruses, including Dengue, Vaccinia, ebolavirus, and marburgvirus, highlighting the multifaceted roles of GAS6 in cellular and infectious contexts.

Verified Bioactivity

Measured by its ability to down regulate the expression of Axl in DU145 human prostate carcinoma cells. The ED50 for this effect is 15-50 ng/mL.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

  • Bioactivity - Cell-Based Assay

    Bioactivity - Cell-Based Assay

    Measured by its ability to down regulate the expression of Axl in DU145 human prostate carcinoma cells. The ED50 for this effect is 27.66 ng/mL. Corresponding to a specific activity is 3.6×104 U/mg.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag C-6*His
  • Accession

    Q14393-2 (A31-A678)

  • Gene ID
    2621
  • Molecular Construction
    • N-term
    • GAS6 (A31-A678)
      Accession # Q14393
    • 6*His
    • C-term
  • Protein Length

    Full Length of Isoform-2

  • Synonyms

    GAS6; AXSF; Prev. AXLLG; DKFZp666G247; AXL Receptor Tyrosine Kinase Ligand; FLJ34709; Growth Arrest-Specific Protein 6; GAS-6; Growth Arrest Specific 6; AXL Stimulatory Factor

  • AA Sequence

    ALLPAREATQFLRPRQRRAFQVFEEAKQGHLERECVEELCSREEAREVFENDPETDYFYPRYLDCINKYGSPYTKNSGFATCVQNLPDQCTPNPCDRKGTQACQDLMGNFFCLCKAGWGGRLCDKDVNECSQENGGCLQICHNKPGSFHCSCHSGFELSSDGRTCQDIDECADSEACGEARCKNLPGSYSCLCDEGFAYSSQEKACRDVDECLQGRCEQVCVNSPGSYTCHCDGRGGLKLSQDMDTCEDILPCVPFSVAKSVKSLYLGRMFSGTPVIRLRFKRLQPTRLVAEFDFRTFDPEGILLFAGGHQDSTWIVLALRAGRLELQLRYNGVGRVTSSGPVINHGMWQTISVEELARNLVIKVNRDAVMKIAVAGDLFQPERGLYHLNLTVGGIPFHEKDLVQPINPRLDGCMRSWNWLNGEDTTIQETVKVNTRMQCFSVTERGSFYPGSGFAFYSLDYMRTPLDVGTESTWEVEVVAHIRPAADTGVLFALWAPDLRAVPLSVALVDYHSTKKLKKQLVVLAVEHTALALMEIKVCDGQEHVVTVSLRDGEATLEVDGTRGQSEVSAAQLQERLAVLERHLRSPVLTFAGGLPDVPVTSAPVTAFYRGCMTLEVNRRLLDLDEAAYKHSDITAHSCPPVEPAAA

  • Predicted Molecular Mass

    71.9 kDa

  • Molecular Weight

    Approximately 75-95 kDa, based on SDS-PAGE under reducing conditions.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured by its ability to down regulate the expression of Axl in DU145 human prostate carcinoma cells. The ED50 for this effect is 27.66 ng/mL. Corresponding to a specific activity is 3.6×104 U/mg.

  • GAS6 Protein, Human (HEK293, His) Product Image
    1/16
  • Experimental Validation Results for GAS6 Protein, Human (HEK293, His)
  • Experimental Validation Results for GAS6 Protein, Human (HEK293, His)
  • GAS6 Protein, Human (HEK293, His) Product Image