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Notch 3 Protein, Human (HEK293, Fc)

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Based on 1 publication(s) in Google Scholar

Notch 3 protein is a receptor for the ligands JAG1, JAG2 and DLL1 and plays a crucial regulatory role in cell fate determination. Upon ligand activation, NICD forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes within the enhancer of the cleavage site. Notch 3 Protein, Human (HEK293, Fc) is the recombinant human-derived Notch3 protein, expressed by HEK293 , with C-hFc labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

Notch 3 protein is a receptor for the ligands JAG1, JAG2 and DLL1 and plays a crucial regulatory role in cell fate determination. Upon ligand activation, NICD forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes within the enhancer of the cleavage site. Notch 3 Protein, Human (HEK293, Fc) is the recombinant human-derived Notch3 protein, expressed by HEK293 , with C-hFc labeled tag.

Background

Notch 3 protein serves as a receptor for membrane-bound ligands, including Jagged1, Jagged2, and Delta1, playing a pivotal role in regulating cell-fate determination. Upon ligand activation, the released notch intracellular domain (NICD) forms a transcriptional activator complex with RBPJ/RBPSUH, initiating the activation of genes within the enhancer of split locus. This multifaceted protein influences cellular differentiation, proliferation, and apoptotic programs. Structurally, it exists as a heterodimer composed of a C-terminal fragment (N(TM)) and a N-terminal fragment (N(EC)), likely linked by disulfide bonds. Notch 3 interacts with transcriptional coactivators MAML1, MAML2, and MAML3, modulating downstream transcriptional processes. It also engages with PSMA1 and HIF1AN, contributing to diverse cellular functions and regulatory pathways.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag C-hFc
  • Accession

    Q9UM47 (A40-E467)

  • Gene ID
    4854  [NCBI]
  • Molecular Construction
    • N-term
    • Notch3 (A40-E467)
      Accession # Q9UM47
    • hFc
    • C-term
  • Protein Length

    Partial Extracellular Domain

  • Synonyms

    NOTCH3; Notch 3-Like Protein; Prev. CADASIL; CADASIL1; Notch 3; CARASIL1; CASIL; FPLD1; Neurogenic Locus Notch Homolog Protein 3; IMF2; Notch (Drosophila) Homolog 3; LMNS; Notch Homolog 3 (Drosophila); Notch Receptor 3; Notch Homolog 3

  • AA Sequence

    APPCLDGSPCANGGRCTQLPSREAACLCPPGWVGERCQLEDPCHSGPCAGRGVCQSSVVAGTARFSCRCPRGFRGPDCSLPDPCLSSPCAHGARCSVGPDGRFLCSCPPGYQGRSCRSDVDECRVGEPCRHGGTCLNTPGSFRCQCPAGYTGPLCENPAVPCAPSPCRNGGTCRQSGDLTYDCACLPGFEGQNCEVNVDDCPGHRCLNGGTCVDGVNTYNCQCPPEWTGQFCTEDVDECQLQPNACHNGGTCFNTLGGHSCVCVNGWTGESCSQNIDDCATAVCFHGATCHDRVASFYCACPMGKTGLLCHLDDACVSNPCHEDAICDTNPVNGRAICTCPPGFTGGACDQDVDECSIGANPCEHLGRCVNTQGSFLCQCGRGYTGPRCETDVNECLSGPCRNQATCLDRIGQFTCICMAGFTGTYCE

  • Predicted Molecular Mass

    71.5 kDa

  • Molecular Weight

    Approximately 65-83 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Purity

    ≥ 95%, as determined by Bis-Tris PAGE.

Product Properties

Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
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