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CHI3L1 Protein, Mouse (HEK293, His)

1 Cited Publications
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Based on 1 publication(s) in Google Scholar

The CHI3L1 protein is a carbohydrate-binding lectin with a preference for chitin that is involved in tissue remodeling and cellular adaptation to environmental changes. It is involved in Th2 inflammation, IL-13-induced inflammation, allergen sensitization, inflammatory cell apoptosis, dendritic cell accumulation, M2 macrophage differentiation and pathogenic intestinal bacterial invasion. CHI3L1 Protein, Mouse (HEK293, His) is the recombinant mouse-derived CHI3L1 protein, expressed by HEK293 , with C-10*His labeled tag.

For research use only. We do not sell to patients.
  • Species: Mouse
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for CHI3L1 Protein, Mouse (HEK293, His)
  • Experimental Validation Results for CHI3L1 Protein, Mouse (HEK293, His)
  • Experimental Validation Results for CHI3L1 Protein, Mouse (HEK293, His)
  • CHI3L1 Protein, Mouse (HEK293, His) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
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  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Use of MCE Products

Biological Activity

Description

The CHI3L1 protein is a carbohydrate-binding lectin with a preference for chitin that is involved in tissue remodeling and cellular adaptation to environmental changes. It is involved in Th2 inflammation, IL-13-induced inflammation, allergen sensitization, inflammatory cell apoptosis, dendritic cell accumulation, M2 macrophage differentiation and pathogenic intestinal bacterial invasion. CHI3L1 Protein, Mouse (HEK293, His) is the recombinant mouse-derived CHI3L1 protein, expressed by HEK293 , with C-10*His labeled tag.

Background

CHI3L1 Protein is a carbohydrate-binding lectin that exhibits a specific preference for chitin. Although it lacks chitinase activity, it plays crucial roles in tissue remodeling and enables cells to adapt and respond to environmental changes. It is involved in various processes, such as T-helper cell type 2 (Th2) inflammatory response, IL-13-induced inflammation, regulation of allergen sensitization, modulation of inflammatory cell apoptosis, dendritic cell accumulation, and promotion of M2 macrophage differentiation. Furthermore, it facilitates the invasion of pathogenic enteric bacteria into the colonic mucosa and lymphoid organs. In colonic epithelial cells, it mediates the activation of the AKT1 signaling pathway, leading to subsequent production of IL8. CHI3L1 also regulates antibacterial responses in the lung by enhancing macrophage bacterial killing, controlling bacterial dissemination, and promoting host tolerance. Additionally, it plays a role in regulating hyperoxia-induced injury, inflammation, and epithelial apoptosis in the lung. It exists as a monomer.

Verified Bioactivity

Measured by the ability of the immobilized protein to support the adhesion of FaDu human squamous cell carcinoma cells. The ED50 for this effect is 3.232 μg/ml, corresponding to a specific activity is 309.4059 units/mg.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

  • Bioactivity - Cell-Based Assay

    Bioactivity - Cell-Based Assay

    Measured by the ability of the immobilized protein to support the adhesion of FaDu human squamous cell carcinoma cells. The ED50 for this effect is 3.232 μg/mL, corresponding to a specific activity is 309.4059 units/mg.

Technical Parameters

  • Species Mouse
  • Source HEK293
  • Tag C-10*His
  • Accession

    Q61362-2 (Y22-A381)

  • Gene ID
    12654  [NCBI]
  • Molecular Construction
    • N-term
    • CHI3L1 (Y22-A381)
      Accession # Q61362-2
    • 10*His
    • C-term
  • Protein Length

    Partial

  • Synonyms

    CHI3L1; HCGP-39; Chitinase 3 Like 1; CGP-39; Chitinase-3-Like Protein 1; YKL-40; YKL40; GP-39; YK-40; CHI3L1 Protein; GP39; HC-Gp39; Chitinase 3-Like 1 (Cartilage Glycoprotein-39); HCGP-3P; Cartilage Glycoprotein-39; YYL-40; Cartilage Glycoprotein 39; ASR

  • AA Sequence

    YKLVCYFTSWSQYREGVGSFLPDAIQPFLCTHIIYSFANISSDNMLSTWEWNDESNYDKLNKLKTRNTNLKTLLSVGGWKFGEKRFSEIASNTERRTAFVRSVAPFLRSYGFDGLDLAWLYPRLRDKQYFSTLIKELNAEFTKEVQPGREKLLLSAALSAGKVAIDTGYDIAQIAQHLDFINLMTYDFHGVWRQITGHHSPLFQGQKDTRFDRYSNVNYAVQYMIRLGAQASKLLMGIPTFGKSFTLASSENQLGAPISGEGLPGRFTKEAGTLAYYEICDFLKGAEVHRLSNEKVPFATKGNQWVGYEDKESVKNKVGFLKEKKLAGAMVWALDLDDFQGTCQPKEFFPLTNAIKDALA

  • Molecular Weight

    Approximately 42-50 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured by the ability of the immobilized protein to support the adhesion of FaDu human squamous cell carcinoma cells. The ED50 for this effect is 3.232 μg/mL, corresponding to a specific activity is 309.4059 units/mg.

  • 1/16

    CHI3L1 Protein, Mouse (HEK293, His) purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Feb;12(6):e2310169. [Abstract]

    Customer Validation
     Cellular CO-IP assays of Chi3L1 (CHI3L1 Protein, Mouse (HEK293, His)) (10 min) and IL-17RA. BLM-SSc WT mice DFs lysates were immunoprecipitated with antibody against IL-17RA, immunoblot with Chi3L1 antibody (B) and vice versa (C).

  • CHI3L1 Protein, Mouse (HEK293, His) Product Image
    1/16
  • Experimental Validation Results for CHI3L1 Protein, Mouse (HEK293, His)
  • Experimental Validation Results for CHI3L1 Protein, Mouse (HEK293, His)
  • Experimental Validation Results for CHI3L1 Protein, Mouse (HEK293, His)
  • CHI3L1 Protein, Mouse (HEK293, His) Product Image

  • CHI3L1 Protein, Mouse (HEK293, His) purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Feb;12(6):e2310169. [Abstract]

    Cellular CO-IP assays of Chi3L1 (CHI3L1 Protein, Mouse (HEK293, His)) (10 min) and IL-17RA. BLM-SSc WT mice DFs lysates were immunoprecipitated with antibody against IL-17RA, immunoblot with Chi3L1 antibody (B) and vice versa (C).