CHI3L1 Protein, Mouse (HEK293, His)

The CHI3L1 protein is a carbohydrate-binding lectin with a preference for chitin that is involved in tissue remodeling and cellular adaptation to environmental changes. It is involved in Th2 inflammation, IL-13-induced inflammation, allergen sensitization, inflammatory cell apoptosis, dendritic cell accumulation, M2 macrophage differentiation and pathogenic intestinal bacterial invasion. CHI3L1 Protein, Mouse (HEK293, His) is the recombinant mouse-derived CHI3L1 protein, expressed by HEK293 , with C-10*His labeled tag.

CHI3L1 Protein is a carbohydrate-binding lectin that exhibits a specific preference for chitin. Although it lacks chitinase activity, it plays crucial roles in tissue remodeling and enables cells to adapt and respond to environmental changes. It is involved in various processes, such as T-helper cell type 2 (Th2) inflammatory response, IL-13-induced inflammation, regulation of allergen sensitization, modulation of inflammatory cell apoptosis, dendritic cell accumulation, and promotion of M2 macrophage differentiation. Furthermore, it facilitates the invasion of pathogenic enteric bacteria into the colonic mucosa and lymphoid organs. In colonic epithelial cells, it mediates the activation of the AKT1 signaling pathway, leading to subsequent production of IL8. CHI3L1 also regulates antibacterial responses in the lung by enhancing macrophage bacterial killing, controlling bacterial dissemination, and promoting host tolerance. Additionally, it plays a role in regulating hyperoxia-induced injury, inflammation, and epithelial apoptosis in the lung. It exists as a monomer.

Measured by the ability of the immobilized protein to support the adhesion of FaDu human squamous cell carcinoma cells. The ED₅₀ for this effect is 3.232 μg/ml, corresponding to a specific activity is 309.4059 units/mg.

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  Measured by the ability of the immobilized protein to support the adhesion of FaDu human squamous cell carcinoma cells. The ED₅₀ for this effect is 3.232 μg/mL, corresponding to a specific activity is 309.4059 units/mg.

Mouse

HEK293

C-10*His

Q61362-2 (Y22-A381)

12654  [NCBI]

Partial

YKLVCYFTSWSQYREGVGSFLPDAIQPFLCTHIIYSFANISSDNMLSTWEWNDESNYDKLNKLKTRNTNLKTLLSVGGWKFGEKRFSEIASNTERRTAFVRSVAPFLRSYGFDGLDLAWLYPRLRDKQYFSTLIKELNAEFTKEVQPGREKLLLSAALSAGKVAIDTGYDIAQIAQHLDFINLMTYDFHGVWRQITGHHSPLFQGQKDTRFDRYSNVNYAVQYMIRLGAQASKLLMGIPTFGKSFTLASSENQLGAPISGEGLPGRFTKEAGTLAYYEICDFLKGAEVHRLSNEKVPFATKGNQWVGYEDKESVKNKVGFLKEKKLAGAMVWALDLDDFQGTCQPKEFFPLTNAIKDALA

Approximately 40.83 kDa

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  ≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.