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  2. Cytokines and Growth Factors Receptor Proteins Enzymes & Regulators
  3. Ephrin/Eph Family Receptor Tyrosine Kinases Protein Tyrosine Kinases
  4. Eph Receptors
  5. EphA4
  6. EphA4 Protein, Mouse (528a.a, HEK293, His)

EphA4 Protein, Mouse (528a.a, HEK293, His)

Cat. No.: HY-P78293
Handling Instructions

The EphA4 protein is a receptor tyrosine kinase that participates in contact-dependent bidirectional signaling with ephrin A and ephrin B ligands. EphA4 is activated by GPI-anchored EFNA and transmembrane EFNB and regulates cell morphology, integrin-dependent adhesion, and GTPase activity. EphA4 Protein, Mouse (528a.a, HEK293, His) is the recombinant mouse-derived EphA4 protein, expressed by HEK293 , with C-His labeled tag. The total length of EphA4 Protein, Mouse (528a.a, HEK293, His) is 528 a.a., with molecular weight of 60-70 kDa.

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Description

The EphA4 protein is a receptor tyrosine kinase that participates in contact-dependent bidirectional signaling with ephrin A and ephrin B ligands. EphA4 is activated by GPI-anchored EFNA and transmembrane EFNB and regulates cell morphology, integrin-dependent adhesion, and GTPase activity. EphA4 Protein, Mouse (528a.a, HEK293, His) is the recombinant mouse-derived EphA4 protein, expressed by HEK293 , with C-His labeled tag. The total length of EphA4 Protein, Mouse (528a.a, HEK293, His) is 528 a.a., with molecular weight of 60-70 kDa.

Background

The EphA4 protein, a receptor tyrosine kinase, engages in contact-dependent bidirectional signaling with membrane-bound ephrin family ligands on adjacent cells. Distinguished by its high promiscuity, EphA4 uniquely binds and is physiologically activated by both GPI-anchored ephrin-A and transmembrane ephrin-B ligands, including EFNA1 and EFNB3. Upon activation by ephrin ligands, EphA4 modulates cell morphology and integrin-dependent cell adhesion through the regulation of Rac, Rap, and Rho GTPases activity. Crucial in the development of the nervous system, EphA4 controls various steps of axonal guidance, including the establishment of corticospinal projections and the segregation of motor and sensory axons during neuromuscular circuit development. In synaptic plasticity, EphA4 participates by phosphorylating CDK5 at 'Tyr-15,' leading to the regulation of RHOA and dendritic spine morphogenesis. Furthermore, EphA4 plays roles in repair after injury by preventing axonal regeneration and in angiogenesis, contributing to central nervous system vascular formation. Its promiscuity extends its involvement in various cell-cell signaling processes, regulating the development of the thymic epithelium and, during the development of the cochlear organ of Corti, facilitating pillar cell separation through the formation of a ternary complex with ADAM10 and CADH1, leading to the cleavage of CADH1 and disruption of adherens junctions. EphA4 also phosphorylates CAPRIN1, promoting CAPRIN1-dependent formation of a membraneless compartment.

Species

Mouse

Source

HEK293

Tag

C-His

Accession

Q03137 (V20-T547)

Gene ID

13838  [NCBI]

Molecular Construction
N-term
EphA4 (V20-T547)
Accession # Q03137
His
C-term
Synonyms
Sek; Sek1; EphA4; HEK8; SEK; TYRO1; EK8
Molecular Weight

60-70 kDa

Purity

Greater than 95% as determined by Tris-Bis PAGE.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Documentation
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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EphA4 Protein, Mouse (528a.a, HEK293, His)
Cat. No.:
HY-P78293
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