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FANCC Protein, Human (His-SUMO)

Cat. No.: HY-P72190
COA Handling Instructions

FANCC Protein repairs and maintains DNA, aids in STAT1 activation, and forms a multisubunit FA complex. It interacts with ZBTB32, STAT1, CDK1, and EIF2AK2. The FA complex excludes FA patients and may include HSP70, while interaction with EIF2AK2 leads to increased activation and cell death. FANCC Protein, Human (His-SUMO) is the recombinant human-derived FANCC protein, expressed by E. coli , with N-6*His, N-SUMO labeled tag. The total length of FANCC Protein, Human (His-SUMO) is 558 a.a., with molecular weight of ~79.4 kDa.

For research use only. We do not sell to patients.

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Description

FANCC Protein repairs and maintains DNA, aids in STAT1 activation, and forms a multisubunit FA complex. It interacts with ZBTB32, STAT1, CDK1, and EIF2AK2. The FA complex excludes FA patients and may include HSP70, while interaction with EIF2AK2 leads to increased activation and cell death. FANCC Protein, Human (His-SUMO) is the recombinant human-derived FANCC protein, expressed by E. coli , with N-6*His, N-SUMO labeled tag. The total length of FANCC Protein, Human (His-SUMO) is 558 a.a., with molecular weight of ~79.4 kDa.

Background

FANCC protein, a crucial component of the Fanconi anemia (FA) complex, is implicated in DNA repair, potentially operating in postreplication repair or functioning within cell cycle checkpoints. It may play a vital role in the repair of interstrand DNA cross-links and contribute to the maintenance of normal chromosome stability. Upon induction by interferon-gamma (IFNG), FANCC may facilitate the activation of STAT1 by recruiting it to IFNGR1. As a part of the multisubunit FA complex, consisting of FANCA, FANCB, FANCC, FANCE, FANCF, FANCG, FANCL/PHF9, and FANCM, it potentially includes HSP70. Importantly, this complex is absent in individuals with Fanconi anemia. FANCC interacts with various proteins, including ZBTB32, STAT1 (especially upon IFNG induction), CDK1, and EIF2AK2. The interaction between FANCC variants and EIF2AK2 may lead to enhanced EIF2AK2 activation and subsequent cell death. These interactions underscore the multifaceted role of FANCC in cellular processes related to DNA repair, signaling, and maintenance of genomic stability.

Species

Human

Source

E. coli

Tag

N-6*His;N-SUMO

Accession

Q00597 (M1-V558)

Gene ID
Molecular Construction
N-term
6*His-SUMO
FANCC (M1-V558)
Accession # Q00597
C-term
Synonyms
bA80I15.1; FA3; FAC; FACC; FANCC; FANCC_HUMAN; Fanconi anemia complementation group C; Fanconi anemia complementation group C protein; Fanconi anemia group C protein; Fanconi pancytopenia type 3; FLJ14675; Protein FACC
AA Sequence

MAQDSVDLSCDYQFWMQKLSVWDQASTLETQQDTCLHVAQFQEFLRKMYEALKEMDSNTVIERFPTIGQLLAKACWNPFILAYDESQKILIWCLCCLINKEPQNSGQSKLNSWIQGVLSHILSALRFDKEVALFTQGLGYAPIDYYPGLLKNMVLSLASELRENHLNGFNTQRRMAPERVASLSRVCVPLITLTDVDPLVEALLICHGREPQEILQPEFFEAVNEAILLKKISLPMSAVVCLWLRHLPSLEKAMLHLFEKLISSERNCLRRIECFIKDSSLPQAACHPAIFRVVDEMFRCALLETDGALEIIATIQVFTQCFVEALEKASKQLRFALKTYFPYTSPSLAMVLLQDPQDIPRGHWLQTLKHISELLREAVEDQTHGSCGGPFESWFLFIHFGGWAEMVAEQLLMSAAEPPTALLWLLAFYYGPRDGRQQRAQTMVQVKAVLGHLLAMSRSSSLSAQDLQTVAGQGTDTDLRAPAQQLIRHLLLNFLLWAPGGHTIAWDVITLMAHTAEITHEIIGFLDQTLYRWNRLGIESPRSEKLARELLKELRTQV

Molecular Weight

Approximately 79.4 kDa

Purity

Greater than 90% as determined by reducing SDS-PAGE.

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.2 μm solution of Tris-based buffer, 50% Glycerol.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US;may vary elsewhere.

Documentation

FANCC Protein, Human (His-SUMO) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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FANCC Protein, Human (His-SUMO)
Cat. No.:
HY-P72190
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