FANCC Protein, Human (His-SUMO)

FANCC Protein repairs and maintains DNA, aids in STAT1 activation, and forms a multisubunit FA complex. It interacts with ZBTB32, STAT1, CDK1, and EIF2AK2. The FA complex excludes FA patients and may include HSP70, while interaction with EIF2AK2 leads to increased activation and cell death. FANCC Protein, Human (His-SUMO) is the recombinant human-derived FANCC protein, expressed by E. coli , with N-6*His, N-SUMO labeled tag.

FANCC protein, a crucial component of the Fanconi anemia (FA) complex, is implicated in DNA repair, potentially operating in postreplication repair or functioning within cell cycle checkpoints. It may play a vital role in the repair of interstrand DNA cross-links and contribute to the maintenance of normal chromosome stability. Upon induction by interferon-gamma (IFNG), FANCC may facilitate the activation of STAT1 by recruiting it to IFNGR1. As a part of the multisubunit FA complex, consisting of FANCA, FANCB, FANCC, FANCE, FANCF, FANCG, FANCL/PHF9, and FANCM, it potentially includes HSP70. Importantly, this complex is absent in individuals with Fanconi anemia. FANCC interacts with various proteins, including ZBTB32, STAT1 (especially upon IFNG induction), CDK1, and EIF2AK2. The interaction between FANCC variants and EIF2AK2 may lead to enhanced EIF2AK2 activation and subsequent cell death. These interactions underscore the multifaceted role of FANCC in cellular processes related to DNA repair, signaling, and maintenance of genomic stability.

Human

E. coli

N-6*His;N-SUMO

Q00597 (M1-V558)

2176  [NCBI]

Full Length

MAQDSVDLSCDYQFWMQKLSVWDQASTLETQQDTCLHVAQFQEFLRKMYEALKEMDSNTVIERFPTIGQLLAKACWNPFILAYDESQKILIWCLCCLINKEPQNSGQSKLNSWIQGVLSHILSALRFDKEVALFTQGLGYAPIDYYPGLLKNMVLSLASELRENHLNGFNTQRRMAPERVASLSRVCVPLITLTDVDPLVEALLICHGREPQEILQPEFFEAVNEAILLKKISLPMSAVVCLWLRHLPSLEKAMLHLFEKLISSERNCLRRIECFIKDSSLPQAACHPAIFRVVDEMFRCALLETDGALEIIATIQVFTQCFVEALEKASKQLRFALKTYFPYTSPSLAMVLLQDPQDIPRGHWLQTLKHISELLREAVEDQTHGSCGGPFESWFLFIHFGGWAEMVAEQLLMSAAEPPTALLWLLAFYYGPRDGRQQRAQTMVQVKAVLGHLLAMSRSSSLSAQDLQTVAGQGTDTDLRAPAQQLIRHLLLNFLLWAPGGHTIAWDVITLMAHTAEITHEIIGFLDQTLYRWNRLGIESPRSEKLARELLKELRTQV

≥ 90%, as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of PBS, 6% trehalose, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.