FAP Protein, Mouse (HEK293, His)

FAP protein is a cell surface glycoprotein serine protease that regulates tissue remodeling, fibrosis, inflammation, and tumor growth. It cleaves post-proline residues, degrades SERPINF2 and SPRY2, and exhibits dipeptidyl peptidase activity. FAP Protein, Mouse (HEK293, His) is the recombinant mouse-derived FAP protein, expressed by HEK293 , with N-His labeled tag. The total length of FAP Protein, Mouse (HEK293, His) is 736 a.a., with molecular weight of 90-110 kDa.

FAP Protein is a cell surface glycoprotein serine protease that is involved in various cellular processes such as tissue remodeling, fibrosis, wound healing, inflammation, and tumor growth. It exhibits endopeptidase activity, particularly cleaving post-proline residues, and can degrade substances like alpha-2-antiplasmin SERPINF2 and SPRY2. FAP Protein also has dipeptidyl peptidase activity, with a preference for specific dipeptide substrates. It interacts with other proteins like DPP4, PLAUR, and integrins to promote pericellular proteolysis of the extracellular matrix, leading to cell adhesion, migration, and invasion. Additionally, FAP Protein plays a role in tissue remodeling, wound healing, and cell invasiveness in malignant melanoma cancers. It also enhances tumor growth by promoting angiogenesis, collagen fiber degradation, and apoptosis while reducing the immune system's antitumor response. In melanocytic cells, FAP Protein acts as a tumor suppressor by regulating cell proliferation and survival independently of its serine protease activity.

1.Measured by its ability to convert the substrate benzyloxycarbonyl-Gly-Pro-7-amido-4-methylcoumarin (Z-GP-AMC) to Z-Gly-Pro and 7-amino-4-methylcoumarin (AMC). The specific activity is >1871.29 pmol/min/µg, as measured under the described conditions.
2.Immobilized Mouse FAP, His Tag at 1 μg/mL (100 μl/well) on the plate. Dose response curve for Anti-FAP Antibody, hFc Tag with the EC₅₀ of 4.6 ng/mL determined by ELISA.

Mouse

HEK293

N-His

P97321-1 (L26-D761)

14089  [NCBI]

LRPSRVYKPEGNTKRALTLKDILNGTFSYKTYFPNWISEQEYLHQSEDDNIVFYNIETRESYIILSNSTMKSVNATDYGLSPDRQFVYLESDYSKLWRYSYTATYYIYDLQNGEFVRGYELPRPIQYLCWSPVGSKLAYVYQNNIYLKQRPGDPPFQITYTGRENRIFNGIPDWVYEEEMLATKYALWWSPDGKFLAYVEFNDSDIPIIAYSYYGDGQYPRTINIPYPKAGAKNPVVRVFIVDTTYPHHVGPMEVPVPEMIASSDYYFSWLTWVSSERVCLQWLKRVQNVSVLSICDFREDWHAWECPKNQEHVEESRTGWAGGFFVSTPAFSQDATSYYKIFSDKDGYKHIHYIKDTVENAIQITSGKWEAIYIFRVTQDSLFYSSNEFEGYPGRRNIYRISIGNSPPSKKCVTCHLRKERCQYYTASFSYKAKYYALVCYGPGLPISTLHDGRTDQEIQVLEENKELENSLRNIQLPKVEIKKLKDGGLTFWYKMILPPQFDRSKKYPLLIQVYGGPCSQSVKSVFAVNWITYLASKEGIVIALVDGRGTAFQGDKFLHAVYRKLGVYEVEDQLTAVRKFIEMGFIDEERIAIWGWSYGGYVSSLALASGTGLFKCGIAVAPVSSWEYYASIYSERFMGLPTKDDNLEHYKNSTVMARAEYFRNVDYLLIHGTADDNVHFQNSAQIAKALVNAQVDFQAMWYSDQNHGISSGRSQNHLYTHMTHFLKQCFSLSD

Approximately 90-110 kDa due to the glycosylation

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  Greater than 95% as determined by Tris-Bis PAGE.

Lyophilized powder

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4 or 50 mM Tris-HCL, 300 mM NaCl, pH 8.0

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.