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  4. FAP Protein, Mouse (HEK293, His)

FAP Protein, Mouse (HEK293, His)

Cat. No.: HY-P77934
COA Handling Instructions

FAP protein is a cell surface glycoprotein serine protease that regulates tissue remodeling, fibrosis, inflammation, and tumor growth. It cleaves post-proline residues, degrades SERPINF2 and SPRY2, and exhibits dipeptidyl peptidase activity. FAP Protein, Mouse (HEK293, His) is the recombinant mouse-derived FAP protein, expressed by HEK293 , with N-His labeled tag. The total length of FAP Protein, Mouse (HEK293, His) is 736 a.a., with molecular weight of 90-110 kDa.

For research use only. We do not sell to patients.

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  • Biological Activity

  • Technical Parameters

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Description

FAP protein is a cell surface glycoprotein serine protease that regulates tissue remodeling, fibrosis, inflammation, and tumor growth. It cleaves post-proline residues, degrades SERPINF2 and SPRY2, and exhibits dipeptidyl peptidase activity. FAP Protein, Mouse (HEK293, His) is the recombinant mouse-derived FAP protein, expressed by HEK293 , with N-His labeled tag. The total length of FAP Protein, Mouse (HEK293, His) is 736 a.a., with molecular weight of 90-110 kDa.

Background

FAP Protein is a cell surface glycoprotein serine protease that is involved in various cellular processes such as tissue remodeling, fibrosis, wound healing, inflammation, and tumor growth. It exhibits endopeptidase activity, particularly cleaving post-proline residues, and can degrade substances like alpha-2-antiplasmin SERPINF2 and SPRY2. FAP Protein also has dipeptidyl peptidase activity, with a preference for specific dipeptide substrates. It interacts with other proteins like DPP4, PLAUR, and integrins to promote pericellular proteolysis of the extracellular matrix, leading to cell adhesion, migration, and invasion. Additionally, FAP Protein plays a role in tissue remodeling, wound healing, and cell invasiveness in malignant melanoma cancers. It also enhances tumor growth by promoting angiogenesis, collagen fiber degradation, and apoptosis while reducing the immune system's antitumor response. In melanocytic cells, FAP Protein acts as a tumor suppressor by regulating cell proliferation and survival independently of its serine protease activity.

Biological Activity

1.Measured by its ability to convert the substrate benzyloxycarbonyl-Gly-Pro-7-amido-4-methylcoumarin (Z-GP-AMC) to Z-Gly-Pro and 7-amino-4-methylcoumarin (AMC). The specific activity is >1871.29 pmol/min/µg, as measured under the described conditions.
2.Immobilized Mouse FAP, His Tag at 1 μg/mL (100 μl/well) on the plate. Dose response curve for Anti-FAP Antibody, hFc Tag with the EC50 of 4.6 ng/mL determined by ELISA.

Species

Mouse

Source

HEK293

Tag

N-His

Accession

P97321-1 (L26-D761)

Gene ID

14089  [NCBI]

Molecular Construction
N-term
His
FAP (L26-D761)
Accession # P97321
C-term
Synonyms
FAP; FAPalpha; SIMP; Seprase; APCE; DPPIV; DPPIVA; FAPA
AA Sequence

LRPSRVYKPEGNTKRALTLKDILNGTFSYKTYFPNWISEQEYLHQSEDDNIVFYNIETRESYIILSNSTMKSVNATDYGLSPDRQFVYLESDYSKLWRYSYTATYYIYDLQNGEFVRGYELPRPIQYLCWSPVGSKLAYVYQNNIYLKQRPGDPPFQITYTGRENRIFNGIPDWVYEEEMLATKYALWWSPDGKFLAYVEFNDSDIPIIAYSYYGDGQYPRTINIPYPKAGAKNPVVRVFIVDTTYPHHVGPMEVPVPEMIASSDYYFSWLTWVSSERVCLQWLKRVQNVSVLSICDFREDWHAWECPKNQEHVEESRTGWAGGFFVSTPAFSQDATSYYKIFSDKDGYKHIHYIKDTVENAIQITSGKWEAIYIFRVTQDSLFYSSNEFEGYPGRRNIYRISIGNSPPSKKCVTCHLRKERCQYYTASFSYKAKYYALVCYGPGLPISTLHDGRTDQEIQVLEENKELENSLRNIQLPKVEIKKLKDGGLTFWYKMILPPQFDRSKKYPLLIQVYGGPCSQSVKSVFAVNWITYLASKEGIVIALVDGRGTAFQGDKFLHAVYRKLGVYEVEDQLTAVRKFIEMGFIDEERIAIWGWSYGGYVSSLALASGTGLFKCGIAVAPVSSWEYYASIYSERFMGLPTKDDNLEHYKNSTVMARAEYFRNVDYLLIHGTADDNVHFQNSAQIAKALVNAQVDFQAMWYSDQNHGISSGRSQNHLYTHMTHFLKQCFSLSD

Molecular Weight

Approximately 90-110 kDa due to the glycosylation

Purity
  • Greater than 95% as determined by Tris-Bis PAGE.
Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4 or 50 mM Tris-HCL, 300 mM NaCl, pH 8.0

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

FAP Protein, Mouse (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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FAP Protein, Mouse (HEK293, His)
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HY-P77934
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