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FCRN-B2M Heterodimer Protein, Human (HEK293, C-His)

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FCGRT is an IgG Fc receptor. Fc receptor expression is up-regulated by pro-inflammatory cytokines TNF and down-regulated by IFN-γ. FCGRT plays a role in regulating IgG and serum albumin conversion. Overexpression of FCGRT is also associated with poor prognosis of tumors. FCRN-B2M Heterodimer Protein, Human (HEK293, C-His) is a recombinant protein dimer complex containing human-derived FCRN-B2M protein, expressed by HEK293 , with C-6*His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for FCRN-B2M Heterodimer Protein, Human (HEK293, C-His)
  • Experimental Validation Results for FCRN-B2M Heterodimer Protein, Human (HEK293, C-His)
  • FCRN-B2M Heterodimer Protein, Human (HEK293, C-His) Product Image
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  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

FCGRT is an IgG Fc receptor. Fc receptor expression is up-regulated by pro-inflammatory cytokines TNF and down-regulated by IFN-γ. FCGRT plays a role in regulating IgG and serum albumin conversion. Overexpression of FCGRT is also associated with poor prognosis of tumors. FCRN-B2M Heterodimer Protein, Human (HEK293, C-His) is a recombinant protein dimer complex containing human-derived FCRN-B2M protein, expressed by HEK293 , with C-6*His labeled tag.

Background

IgG receptor FcRn large subunit p51 is an IgG Fc receptor with a molecular structure similar to MHC Class I and also binds to β-2 microglobulin. In rodents, FcRn was originally thought to be a receptor that trantranks maternal immunoglobulin G (IgG) from mother to newborn offspring via breast milk and is therefore known as a neonatal Fc receptor. FcRn has also been shown to play a role in regulating IgG and serum albumin conversion, and neonatal Fc receptor expression is up-regulated by pro-inflammatory cytokines TNF and down-regulated by IFN-γ. In the acidic endosomes of endothelial and hematopoietic cells, monomer IgG binds to FcRn, circulates IgG to the cell surface, where it is released into the circulation, regulating, in addition to IgG, homeostasis of the circulating protein albumin /ALB. The up-regulated expression of FCGRT in bladder cancer may serve as a key neutrophil gene associated with poor prognosis. FCGRT overexpression was also associated with decreased PD-L1 expression and decreased tumor mutation load (TMB) level[1][2][3][4][5][6].

Verified Bioactivity

Measured by its binding ability in a functional ELISA. When FCRN-B2M is immobilized at 2.00 µg/mL (100 µL/well), can bind  Biotinylated Human lgG1. The ED50 for this effect is <2.5 μg/mL.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

  • Bioactivity - ELISA

    Bioactivity - ELISA

    Measured by its binding ability in a functional ELISA. When FCRN-B2M is immobilized at 2.00 µg/mL (100 µL/well) can bind Biotinylated Human lgG1. The ED50 for this effect is 199.7 ng/mL.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag C-6*His
  • Accession

    AAF72596 (A24-S297)&P61769 (I21-M119)

  • Gene ID
    2217  [NCBI]&567  [NCBI]
  • Synonyms

    FCGRT; Major Histocompatibility Complex Class I-Like Fc Receptor; Fc Gamma Receptor And Transporter; Neonatal Crystallizable Fc Receptor FcRn Splice Variant 6; Neonatal Fc Receptor; Neonatal Crystallizable Fc Receptor FcRn Splice Variant 5; FcRn; Neonatal

  • AA Sequence

    A1:
    AESHLSLLYHLTAVSSPAPGTPAFWVSGWLGPQQYLSYNSLRGEAEPCGAWVWENQVSWYWEKETTDLRIKEKLFLEAFKALGGKGPYTLQGLLGCELGPDNTSVPTAKFALNGEEFMNFDLKQGTWGGDWPEALAISQRWQQQDKAANKELTFLLFSCPHRLREHLERGRGNLEWKEPPSMRLKARPSSPGFSVLTCSAFSFYPPELQLRFLRNGLAAGTGQGDFGPNSDGSFHASSSLTVKSGDEHHYCCIVQHAGLAQPLRVELESPAKSS
    A2:
    IQRTPKIQVYSRHPAENGKSNFLNCYVSGFHPSDIEVDLLKNGERIEKVEHSDLSFSKDWSFYLLYYTEFTPTEKDEYACRVNHVTLSQPKIVKWDRDM

  • Molecular Weight

    Approximately 37 kDa & 13 kDa, based on SDS-PAGE under reducing conditions.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder

Formulation

1.Lyophilized from a 0.22 μm filtered solution of 50 mM HEPES, 150 mM NaCl, pH 7.4.
2.Lyophilized from a 0.22 μm filtered solution of 10 mM HEPES, 100 mM KCl, 50% Glycerol, pH 7.4.
3.Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
4.Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.
Please refer to the lot-specific COA for specific buffer information.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured by its binding ability in a functional ELISA. When FCRN-B2M is immobilized at 2.00 µg/mL (100 µL/well) can bind Biotinylated Human lgG1. The ED50 for this effect is 199.7 ng/mL.

  • FCRN-B2M Heterodimer Protein, Human (HEK293, C-His) Product Image
    1/16
  • Experimental Validation Results for FCRN-B2M Heterodimer Protein, Human (HEK293, C-His)
  • Experimental Validation Results for FCRN-B2M Heterodimer Protein, Human (HEK293, C-His)
  • FCRN-B2M Heterodimer Protein, Human (HEK293, C-His) Product Image