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FLT3 Protein, Human (T227M, HEK293, His)

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Based on 1 Customer Validation

FLT3 is a tyrosine-protein kinase receptor that regulates differentiation, proliferation, and survival of hematopoietic progenitor cells and dendritic cells. It phosphorylates downstream effectors such as SHC1 and AKT1, activating signaling cascades involving MTOR, RAS, and MAP kinases. FLT3 Protein, Human (T227M, HEK293, His) is the recombinant human-derived FLT3 protein, expressed by HEK293 , with C-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for FLT3 Protein, Human (T227M, HEK293, His)
  • Experimental Validation Results for FLT3 Protein, Human (T227M, HEK293, His)
  • Experimental Validation Results for FLT3 Protein, Human (T227M, HEK293, His)
  • Experimental Validation Results for FLT3 Protein, Human (T227M, HEK293, His)
  • Experimental Validation Results for FLT3 Protein, Human (T227M, HEK293, His)
  • FLT3 Protein, Human (T227M, HEK293, His) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

FLT3 is a tyrosine-protein kinase receptor that regulates differentiation, proliferation, and survival of hematopoietic progenitor cells and dendritic cells. It phosphorylates downstream effectors such as SHC1 and AKT1, activating signaling cascades involving MTOR, RAS, and MAP kinases. FLT3 Protein, Human (T227M, HEK293, His) is the recombinant human-derived FLT3 protein, expressed by HEK293 , with C-His labeled tag.

Background

FLT3, a tyrosine-protein kinase, functions as a cell-surface receptor for the cytokine FLT3LG, exerting regulatory control over the differentiation, proliferation, and survival of hematopoietic progenitor cells and dendritic cells. This receptor facilitates the phosphorylation of various downstream effectors, including SHC1 and AKT1, and activates signaling cascades involving MTOR, RAS, MAPK1/ERK2, and/or MAPK3/ERK1. Moreover, it plays a pivotal role in the phosphorylation of FES, FER, PTPN6/SHP, PTPN11/SHP-2, PLCG1, and STAT5A and/or STAT5B. While wild-type FLT3 activation leads to modest STAT5A or STAT5B activation, mutations causing constitutive kinase activity result in heightened cell proliferation and resistance to apoptosis, underscoring its role in fostering aberrant signaling pathways.

Verified Bioactivity

1.This product does not contain protein kinase domain.
2.Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human Flt-3 Ligand at 10 μg/mL can bind Biotinylated Human Flt-3. The ED50 for this effect is 0.1-1.0 μg/mL.
3.Loaded Anti-FLT3 Antibody (AGS62P) (HY-P990786) on AHC2 biosensor, can bind FLT3 Protein, Human (T227M, HEK293, His) with an affinity constant of 2.203E-08 M as determined in BLI assay.
4.Loaded Flt-3L-Ig (hum/hum, HY-P991208) on AHC2 biosensor, can bind FLT3 Protein, Human (T227M, HEK293, His) with an affinity constant of 2.041E-08 M as determined in BLI assay.
5.Loaded IMC-EB10 (HY-P991641) on ACH2 biosensor, can bind FLT3 Protein, Human (T227M, HEK293, His) with an affinity constant of 4.903E-07 M as determined in BLI assay.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

  • Bioactivity - ELISA

    Bioactivity - ELISA

    Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human Flt-3 Ligand at 10 μg/mL can bind Biotinylated Human Flt-3 .The ED50 for this effect is 0.3422 μg/mL.

  • Bioactivity - BLI

    Bioactivity - BLI

    Loaded Anti-FLT3 Antibody (AGS62P) (HY-P990786) on AHC2 biosensor, can bind FLT3 Protein, Human (T227M, HEK293, His) with an affinity constant of 2.203E-08 M as determined in BLI assay.

  • Bioactivity - BLI

    Bioactivity - BLI

    Loaded Flt-3L-Ig (hum/hum, HY-P991208) on AHC2 biosensor, can bind FLT3 Protein, Human (T227M, HEK293, His) with an affinity constant of 2.041E-08 M as determined in BLI assay.

  • Bioactivity - BLI

    Bioactivity - BLI

    Loaded IMC-EB10 (HY-P991641) on ACH2 biosensor, can bind FLT3 Protein, Human (T227M, HEK293, His) with an affinity constant of 4.903E-07 M as determined in BLI assay.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag C-10*His
  • Accession

    P36888-1 (N27-N541, T227M)

  • Gene ID
    2322  [NCBI]
  • Molecular Construction
    • N-term
    • FLT3 (N27-N541, T227M)
      Accession # P36888-1
    • His
    • C-term
  • Protein Length

    Extracellular Domain

  • Synonyms

    FLT3; CD135 Antigen; Fms Related Receptor Tyrosine Kinase 3; FLK-2; CD135; Growth Factor Receptor Tyrosine Kinase Type III; STK1; Receptor Protein-Tyrosine Kinase; FLK2; Fms-Related Tyrosine Kinase 3; Fms-Like Tyrosine Kinase 3; Fetal Liver Kinase 2; Rece

  • AA Sequence

    NQDLPVIKCVLINHKNNDSSVGKSSSYPMVSESPEDLGCALRPQSSGTVYEAAAVEVDVSASITLQVLVDAPGNISCLWVFKHSSLNCQPHFDLQNRGVVSMVILKMTETQAGEYLLFIQSEATNYTILFTVSIRNTLLYTLRRPYFRKMENQDALVCISESVPEPIVEWVLCDSQGESCKEESPAVVKKEEKVLHELFGMDIRCCARNELGRECTRLFTIDLNQTPQTTLPQLFLKVGEPLWIRCKAVHVNHGFGLTWELENKALEEGNYFEMSTYSTNRTMIRILFAFVSSVARNDTGYYTCSSSKHPSQSALVTIVEKGFINATNSSEDYEIDQYEEFCFSVRFKAYPQIRCTWTFSRKSFPCEQKGLDNGYSISKFCNHKHQPGEYIFHAENDDAQFTKMFTLNIRRKPQVLAEASASQASCFSDGYPLPSWTWKKCSDKSPNCTEEITEGVWNRKANRKVFGQWVSSSTLNMSEAIKGFLVKCCAYNSLGTSCETILLNSPGPFPFIQDN

  • Molecular Weight

    Approximately 85-95 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human Flt-3 Ligand at 10 μg/mL can bind Biotinylated Human Flt-3 .The ED50 for this effect is 0.3422 μg/mL.

  • 1/16

    MedChemExpress Validation
     Loaded Anti-FLT3 Antibody (AGS62P) (HY-P990786) on AHC2 biosensor, can bind FLT3 Protein, Human (T227M, HEK293, His) with an affinity constant of 2.203E-08 M as determined in BLI assay.

  • 1/16

    MedChemExpress Validation
     Loaded Flt-3L-Ig (hum/hum, HY-P991208) on AHC2 biosensor, can bind FLT3 Protein, Human (T227M, HEK293, His) with an affinity constant of 2.041E-08 M as determined in BLI assay.

  • 1/16

    MedChemExpress Validation
     Loaded IMC-EB10 (HY-P991641) on ACH2 biosensor, can bind FLT3 Protein, Human (T227M, HEK293, His) with an affinity constant of 4.903E-07 M as determined in BLI assay.

  • FLT3 Protein, Human (T227M, HEK293, His) Product Image
    1/16
  • Experimental Validation Results for FLT3 Protein, Human (T227M, HEK293, His)
  • Experimental Validation Results for FLT3 Protein, Human (T227M, HEK293, His)
  • Experimental Validation Results for FLT3 Protein, Human (T227M, HEK293, His)
  • Experimental Validation Results for FLT3 Protein, Human (T227M, HEK293, His)
  • Experimental Validation Results for FLT3 Protein, Human (T227M, HEK293, His)
  • FLT3 Protein, Human (T227M, HEK293, His) Product Image