Follistatin-like 1/FSTL1 Protein, Mouse (HEK293, His)

Follistatin-like 1 (FSTL1) is a secreted glycoprotein that plays multiple roles in angiogenesis, immune regulation, and cell differentiation. It positively affects the development of the central nervous system, skeletal system, lungs and ureters. Follistatin-like 1/FSTL1 Protein, Mouse (HEK293, His) is the recombinant mouse-derived Follistatin-like 1/FSTL1 protein, expressed by HEK293 , with C-6*His labeled tag.

Follistatin-like 1 (FSTL1) is a secreted glycoprotein with diverse roles in various physiological processes, including angiogenesis, immune response regulation, and cell proliferation and differentiation. It actively participates in the development of the central nervous system, skeletal system, lungs, and ureter. FSTL1 exerts its influence on endothelial cells by promoting survival, migration, and differentiation into network structures through an AKT-dependent mechanism, while also supporting the survival of cardiac myocytes. The glycoprotein initiates signaling cascades by activating different cell surface receptors, including DIP2A, TLR4, and BMP receptors. FSTL1 forms homodimers and interacts with SCN10A, DIP2A (acting as a potential cell surface receptor), BMP4, and CD14, with the latter interaction promoting TLR4-mediated signaling cascades. These interactions highlight the multifaceted nature of FSTL1 in orchestrating various cellular functions and signaling pathways.

Measured by its ability to inhibit the proliferation of OVCAR-3 cells. The ED₅₀ this effect is 15.17 ng/mL, corresponding to a specific activity is 6.592×10⁴ units/mg.

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  Measured by its ability to inhibit the proliferation of OVCAR-3 cells. The ED₅₀ for this effect is 15.17 ng/mL, corresponding to a specific activity is 6.592×10⁴ units/mg.

Mouse

HEK293

C-6*His

Q62356 (E19-I306)

14314  [NCBI]

Full Length of Mature Protein

EEEPRSKSKICANVFCGAGRECAVTEKGEPTCLCIEQCKPHKRPVCGSNGKTYLNHCELHRDACLTGSKIQVDYDGHCKEKKSASPSASPVVCYQANRDELRRRLIQWLEAEIIPDGWFSKGSNYSEILDKYFKSFDNGDSHLDSSEFLKFVEQNETAINITTYADQENNKLLRSLCVDALIELSDENADWKLSFQEFLKCLNPSFNPPEKKCALEDETYADGAETEVDCNRCVCSCGHWVCTAMTCDGKNQKGVQTHTEEEKTGYVQELQKHQGTAEKTKKVNTKEI

Approximately 41-58 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

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  ≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder.

1.Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
2.Lyophilized from a 0.22 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.
Please refer to the lot-specific COA for specific buffer information.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.