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Follistatin-like 1/FSTL1 Protein, Mouse (HEK293, His)

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Follistatin-like 1 (FSTL1) is a secreted glycoprotein that plays multiple roles in angiogenesis, immune regulation, and cell differentiation. It positively affects the development of the central nervous system, skeletal system, lungs and ureters. Follistatin-like 1/FSTL1 Protein, Mouse (HEK293, His) is the recombinant mouse-derived Follistatin-like 1/FSTL1 protein, expressed by HEK293 , with C-6*His labeled tag.

For research use only. We do not sell to patients.
  • Species: Mouse
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for Follistatin-like 1/FSTL1 Protein, Mouse (HEK293, His)
  • Experimental Validation Results for Follistatin-like 1/FSTL1 Protein, Mouse (HEK293, His)
  • Follistatin-like 1/FSTL1 Protein, Mouse (HEK293, His) Product Image
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  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

Follistatin-like 1 (FSTL1) is a secreted glycoprotein that plays multiple roles in angiogenesis, immune regulation, and cell differentiation. It positively affects the development of the central nervous system, skeletal system, lungs and ureters. Follistatin-like 1/FSTL1 Protein, Mouse (HEK293, His) is the recombinant mouse-derived Follistatin-like 1/FSTL1 protein, expressed by HEK293 , with C-6*His labeled tag.

Background

Follistatin-like 1 (FSTL1) is a secreted glycoprotein with diverse roles in various physiological processes, including angiogenesis, immune response regulation, and cell proliferation and differentiation. It actively participates in the development of the central nervous system, skeletal system, lungs, and ureter. FSTL1 exerts its influence on endothelial cells by promoting survival, migration, and differentiation into network structures through an AKT-dependent mechanism, while also supporting the survival of cardiac myocytes. The glycoprotein initiates signaling cascades by activating different cell surface receptors, including DIP2A, TLR4, and BMP receptors. FSTL1 forms homodimers and interacts with SCN10A, DIP2A (acting as a potential cell surface receptor), BMP4, and CD14, with the latter interaction promoting TLR4-mediated signaling cascades. These interactions highlight the multifaceted nature of FSTL1 in orchestrating various cellular functions and signaling pathways.

Verified Bioactivity

1.Measured by its ability to inhibit the proliferation of OVCAR-3 cells. The ED50 this effect is 15.17 ng/mL, corresponding to a specific activity is 6.592×104 units/mg.
2.Measured by its binding ability in a functional ELISA. Immobilized Mouse FSTL1 at 5 μg/mL (100 μL/well) can bind Biotinylated Mouse BMP-4. The ED50 for this effect is 70-110 ng/mL.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

  • Bioactivity - Cell-Based Assay

    Bioactivity - Cell-Based Assay

    Measured by its ability to inhibit the proliferation of OVCAR-3 cells. The ED50 for this effect is 15.17 ng/mL, corresponding to a specific activity is 6.592×104 units/mg.

Technical Parameters

  • Species Mouse
  • Source HEK293
  • Tag C-6*His
  • Accession

    Q62356 (E19-I306)

  • Gene ID
    14314  [NCBI]
  • Molecular Construction
    • N-term
    • FSTL1 (E19-I306)
      Accession # Q62356
    • 6*His
    • C-term
  • Protein Length

    Full Length of Mature Protein

  • Synonyms

    FSTL1; OCC-1; Follistatin Like 1; Tsc36; FRP; FSL1; Follistatin-Related Protein 1; OCC1; Follistatin-Like Protein 1; Follistatin-Like 1

  • AA Sequence

    EEEPRSKSKICANVFCGAGRECAVTEKGEPTCLCIEQCKPHKRPVCGSNGKTYLNHCELHRDACLTGSKIQVDYDGHCKEKKSASPSASPVVCYQANRDELRRRLIQWLEAEIIPDGWFSKGSNYSEILDKYFKSFDNGDSHLDSSEFLKFVEQNETAINITTYADQENNKLLRSLCVDALIELSDENADWKLSFQEFLKCLNPSFNPPEKKCALEDETYADGAETEVDCNRCVCSCGHWVCTAMTCDGKNQKGVQTHTEEEKTGYVQELQKHQGTAEKTKKVNTKEI

  • Predicted Molecular Mass

    33.5 kDa

  • Molecular Weight

    Approximately 45-58 kDa, based on SDS-PAGE under reducing conditions.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

1.Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
2.Lyophilized from a 0.22 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.
Please refer to the lot-specific COA for specific buffer information.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured by its ability to inhibit the proliferation of OVCAR-3 cells. The ED50 for this effect is 15.17 ng/mL, corresponding to a specific activity is 6.592×104 units/mg.

  • Follistatin-like 1/FSTL1 Protein, Mouse (HEK293, His) Product Image
    1/16
  • Experimental Validation Results for Follistatin-like 1/FSTL1 Protein, Mouse (HEK293, His)
  • Experimental Validation Results for Follistatin-like 1/FSTL1 Protein, Mouse (HEK293, His)
  • Follistatin-like 1/FSTL1 Protein, Mouse (HEK293, His) Product Image