Galectin-1/LGALS1 Protein, Human

Galectin-1/LGALS1 is a lectin that binds β-galactoside and complex carbohydrates and regulates apoptosis, proliferation, and differentiation. It inhibits CD45 phosphatase activity, blocks Lyn kinase dephosphorylation, and induces T cell apoptosis. Galectin-1/LGALS1 Protein, Human is the recombinant human-derived Galectin-1/LGALS1 protein, expressed by E. coli , with tag free.

The Galectin-1/LGALS1 protein serves as a lectin with the ability to bind beta-galactoside and a diverse range of complex carbohydrates. It plays a pivotal role in the regulation of apoptosis, cell proliferation, and cell differentiation. Galectin-1/LGALS1 exerts its influence by inhibiting the protein phosphatase activity of CD45, consequently impeding the dephosphorylation of Lyn kinase. Additionally, it acts as a potent inducer of T-cell apoptosis. Existing as a homodimer, Galectin-1/LGALS1 forms interactions with a variety of cellular entities, including CD2, CD3, CD4, CD6, CD7, CD43, ALCAM, and CD45. It also binds LGALS3BP, laminin (via poly-N-acetyllactosamine), and SUSD2. Notably, Galectin-1/LGALS1 engages in an interaction with the cargo receptor TMED10, facilitating translocation from the cytoplasm into the endoplasmic reticulum-Golgi intermediate compartment (ERGIC) and subsequent secretion.

1.Measured by its ability to agglutinate human red blood cells and the ED₅₀ is typically 3-30 μg/mL.
2.Measured in a cell proliferation assay using ARPE-19 cells. The ED₅₀ for this effect is 0.2112-0.3412 μg/mL.

• 
  Measured in a cell proliferation assay using ARPE-19 cells. The ED₅₀ for this effect is 0.3052 μg/mL, corresponding to a specific activity is 3276.54 units/mg.

Human

E. coli

Tag Free

P09382/NP_002296.1 (A2-D135)

3956  [NCBI]

Full Length of Mature Protein

ACGLVASNLNLKPGECLRVRGEVAPDAKSFVLNLGKDSNNLCLHFNPRFNAHGDANTIVCNSKDGGAWGTEQREAVFPFQPGSVAEVCITFDQANLTVKLPDGYEFKFPNRLNLEAINYMAADGDFKIKCVAFD

Approximately 15 kDa

• 
  ≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder

1.Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 5% trehalose, 5% mannitol, 0.01% tween 80.
2.Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.
Please refer to the lot-specific COA for specific buffer information.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.