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IKBKB Protein, Human (HEK293, His, Strep)

IKKβ protein is a serine kinase that is critical in the NF-kappa-B signaling pathway and can respond to various stimuli such as cytokines or cellular stress. As part of the classical IKK complex, IKKβ activates NF-kappa-B through phosphorylation inhibitors, leading to its degradation and release of NF-kappa-B for gene transcription. IKBKB Protein, Human (HEK293, His, Strep) is the recombinant IKBKB, expressed by HEK293, with His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • IKBKB Protein, Human (HEK293, His, Strep) Product Image
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  • Biological Activity
  • Technical Parameters
  • Product Properties
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Biological Activity

Description

IKKβ protein is a serine kinase that is critical in the NF-kappa-B signaling pathway and can respond to various stimuli such as cytokines or cellular stress. As part of the classical IKK complex, IKKβ activates NF-kappa-B through phosphorylation inhibitors, leading to its degradation and release of NF-kappa-B for gene transcription. IKBKB Protein, Human (HEK293, His, Strep) is the recombinant IKBKB, expressed by HEK293, with His labeled tag.

Background

IKKβ protein, a serine kinase, plays a pivotal role in the NF-kappa-B signaling pathway, activated by diverse stimuli such as inflammatory cytokines, bacterial or viral products, DNA damage, or cellular stresses. As a constituent of the canonical IKK complex, IKKβ contributes to the conventional NF-kappa-B activation pathway. It phosphorylates inhibitors of NF-kappa-B on critical serine residues, facilitating their polyubiquitination and subsequent degradation by the proteasome. This orchestrated modification unleashes free NF-kappa-B, allowing its translocation into the nucleus and activation of the transcription of numerous genes involved in immune response, growth control, or protection against apoptosis. Beyond NF-kappa-B inhibitors, IKKβ phosphorylates various signaling pathway components, including NEMO/IKBKG, NF-kappa-B subunits RELA and NFKB1, as well as IKK-related kinases TBK1 and IKBKE. These phosphorylations exert a negative regulation on canonical IKKs and may prevent the excessive production of inflammatory mediators. Moreover, IKKβ targets substrates such as FOXO3, NAA10, NCOA3, BCL10, IRS1, RIPK1, and IRF5, influencing diverse cellular processes and responses.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag N-His;N-Strep
  • Accession

    O14920-1 (M1-S756)

  • Gene ID
    3551
  • Molecular Construction
    • N-term
    • His-Strep
    • O14920-1 (MO14920-1)
      Accession # O14920-1
    • C-term
  • Protein Length

    Full Length of Isoform-1

  • Synonyms

    IKBKB; IKK-2; Inhibitor Of Nuclear Factor Kappa B Kinase Subunit Beta; CDNA FLJ33978 Fis, Clone DFNES2004354, Highly Similar To Inhibitor Of Nuclear Factor Kappa B Kinase Subunit Beta; IKK-Beta; Inhibitor Of Kappa Light Polypeptide Gene Enhancer In B-Cells, Kinase Beta, Isoform CRA_a; NFKBIKB; Nuclear Factor Kappa B Kinase Subunit Beta; IKK2; IkappaB Kinase; IKKB; IKBKB Protein; Inhibitor Of Nuclear Factor Kappa-B Kinase Subunit Beta; IMD15A; Nuclear Factor NF-Kappa-B Inhibitor Kinase Beta; IMD15B; Serine/Threonine Protein Kinase IKBKB; IMD15; I-Kappa-B Kinase 2; IkBKB; Inhibitor Of Kappa Light Polypeptide Gene Enhancer In B-Cells, Kinase Beta; IKK-B; I-Kappa-B-Kinase Beta

  • AA Sequence

    MSWSPSLTTQTCGAWEMKERLGTGGFGNVIRWHNQETGEQIAIKQCRQELSPRNRERWCLEIQIMRRLTHPNVVAARDVPEGMQNLAPNDLPLLAMEYCQGGDLRKYLNQFENCCGLREGAILTLLSDIASALRYLHENRIIHRDLKPENIVLQQGEQRLIHKIIDLGYAKELDQGSLCTSFVGTLQYLAPELLEQQKYTVTVDYWSFGTLAFECITGFRPFLPNWQPVQWHSKVRQKSEVDIVVSEDLNGTVKFSSSLPYPNNLNSVLAERLEKWLQLMLMWHPRQRGTDPTYGPNGCFKALDDILNLKLVHILNMVTGTIHTYPVTEDESLQSLKARIQQDTGIPEEDQELLQEAGLALIPDKPATQCISDGKLNEGHTLDMDLVFLFDNSKITYETQISPRPQPESVSCILQEPKRNLAFFQLRKVWGQVWHSIQTLKEDCNRLQQGQRAAMMNLLRNNSCLSKMKNSMASMSQQLKAKLDFFKTSIQIDLEKYSEQTEFGITSDKLLLAWREMEQAVELCGRENEVKLLVERMMALQTDIVDLQRSPMGRKQGGTLDDLEEQARELYRRLREKPRDQRTEGDSQEMVRLLLQAIQSFEKKVRVIYTQLSKTVVCKQKALELLPKVEEVVSLMNEDEKTVVRLQEKRQKELWNLLKIACSKVRGPVSGSPDSMNASRLSQPGQLMSQPSTASNSLPEPAKKSEELVAEAHNLCTLLENAIQDTVREQDQSFTALDWSWLQTEEEEHSCLEQAS

  • Predicted Molecular Mass

    89.7 KDa

  • Purity

    ≥ 90%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Solution

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

Please use rapid thawing with running water to thaw the protein.

Shipping

Shipping with dry ice.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • IKBKB Protein, Human (HEK293, His, Strep) Product Image
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  • IKBKB Protein, Human (HEK293, His, Strep) Product Image