IL-4 Protein, Human (Biotinylated, HEK293, His-Avi)

The IL-4 protein is primarily secreted by mast cells, T cells, eosinophils, and basophils and is critical for antibody production, hematopoiesis, and immune responses. It induces MHC class II expression on B cells, enhances IgE and IgG1 secretion, and modulates CD23 and IL31RA expression. IL-4 Protein, Human (Biotinylated, HEK293, His-Avi) is the recombinant human-derived IL-4 protein, expressed by HEK293 , with C-Avi, C-His labeled tag. The total length of IL-4 Protein, Human (Biotinylated, HEK293, His-Avi) is 129 a.a., with molecular weight of 23-25 kDa.

The cytokine IL-4, primarily secreted by mast cells, T-cells, eosinophils, and basophils, plays a crucial role in regulating antibody production, hematopoiesis, inflammation, and the development of effector T-cell responses. IL-4 induces the expression of class II MHC molecules on resting B-cells and enhances both the secretion and cell surface expression of IgE and IgG1, contributing to immune responses. Additionally, IL-4 regulates the expression of the low-affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes and positively regulates IL31RA expression in macrophages. Furthermore, IL-4 stimulates autophagy in dendritic cells by interfering with mTORC1 signaling and inducing RUFY4. Beyond its immunological functions, IL-4 plays a critical role in higher functions of the normal brain, such as memory and learning. Upon binding to its receptor, IL-4R, IL-4 initiates signaling through two types of receptor complexes, type 1 mainly on hematopoietic cells and type 2 on nonhematopoietic cells, activating JAK3 and to a lesser extent JAK1 phosphorylation, leading to the activation of the signal transducer and activator of transcription 6/STAT6. IL-4 interacts with both IL-4R and IL13RA1 to mediate its diverse physiological effects.

Immobilized Biotinylated Human IL-4 His at 0.5 μg/mL (100μL/Well) on the plate. Dose response curve for Human IL-4R alpha hFc with the EC₅₀ <25 ng/mL determined by ELISA.

Human

HEK293

C-Avi;C-His

P05112 (H25-S153)

3565  [NCBI]

23-25 kDa

Greater than 95% as determined by Tris-Bis PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Normally 5% trehalose is added as protectant before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.