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Integrin alpha 8 beta 1 Protein, Human (HEK293, Flag, His)

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Based on 1 Customer Validation

Integrin α-8/β-1 coordinates the recruitment of mesenchymal cells during renal organogenesis and recognizes RGD sequences in ligands such as TNC, FN1, SPP1, TGFB1, TGFB3, and VTN. It plays a role in multiple cellular interactions critical for organogenesis, including NPNT as a functional ligand in nephrogenesis. Integrin alpha 8 beta 1 Protein, Human (HEK293, Flag, His) is a recombinant protein dimer complex containing human-derived Integrin alpha 8 beta 1 protein, expressed by HEK293 , with C-10*His, C-Flag labeled tag. Integrin alpha 8 beta 1 Protein, Human (HEK293, Flag, His), has molecular weight of ~85-140 kDa.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for Integrin alpha 8 beta 1 Protein, Human (HEK293, Flag, His)
  • Integrin alpha 8 beta 1 Protein, Human (HEK293, Flag, His) Product Image
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Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
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Biological Activity

Description

Integrin α-8/β-1 coordinates the recruitment of mesenchymal cells during renal organogenesis and recognizes RGD sequences in ligands such as TNC, FN1, SPP1, TGFB1, TGFB3, and VTN. It plays a role in multiple cellular interactions critical for organogenesis, including NPNT as a functional ligand in nephrogenesis. Integrin alpha 8 beta 1 Protein, Human (HEK293, Flag, His) is a recombinant protein dimer complex containing human-derived Integrin alpha 8 beta 1 protein, expressed by HEK293 , with C-10*His, C-Flag labeled tag. Integrin alpha 8 beta 1 Protein, Human (HEK293, Flag, His), has molecular weight of ~85-140 kDa.

Background

The Integrin alpha-8/beta-1 protein plays a crucial role in organogenesis, particularly in the development of the kidney and potentially other organs, by orchestrating the recruitment of mesenchymal cells into epithelial structures. Recognizing the R-G-D sequence within a broad spectrum of ligands, including TNC, FN1, SPP1, TGFB1, TGFB3, and VTN, Integrin alpha-8/beta-1 is implicated in diverse cellular interactions critical for organ formation. In kidney genesis, NPNT is identified as its likely functional ligand. Acting as a neuronal receptor for TNC, it regulates cell-cell interactions and modulates the neurite outgrowth of sensory and motor neurons. Structurally, Integrin alpha-8/beta-1 forms a heterodimer, with the alpha subunit comprising a heavy and a light chain linked by a disulfide bond. Specifically, the alpha-8 subunit associates with the beta-1 subunit, underscoring its integral role in mediating cell-matrix and cell-cell interactions during organ development and neural processes.

Verified Bioactivity

Measured by its binding ability in a functional ELISA. Immobilized Human Fibronectin, at 5 μg/mL (100 μL/well) can bind Biotinylated Human Integrin alpha 8 beta 1 protein. The ED50 for this effect is 428.7 ng/mL

MCE Validation Data

  • Bioactivity - ELISA

    Bioactivity - ELISA

    Measured by its binding ability in a functional ELISA. Immobilized Human Fibronectin, at 5 μg/mL (100 μL/well) can bind Biotinylated Human Integrin alpha 8 beta 1 protein. The ED50 for this effect is 428.7 ng/mL .

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag C-10*His;C-Flag
  • Accession

    P53708 (F39-L1012)&P05556-1 (Q21-D728)

  • Gene ID
    8516  [NCBI]&3688  [NCBI]
  • Synonyms

    ITGA8; Integrin, Alpha 8; Integrin Subunit Alpha 8; Integrin Alpha-8; ITGB1; Very Late Activation Protein, Beta Polypeptide; Prev. MSK12; Integrin VLA-4 Beta Subunit; Prev. FNRB; VLA-4 Subunit Beta; Prev. MDF2; Integrin Beta 1; GPIIA; Integrin Beta; Integ

  • AA Sequence

    FNLDVEKLTVYSGPKGSYFGYAVDFHIPDARTASVLVGAPKANTSQPDIVEGGAVYYCPWPAEGSAQCRQIPFDTTNNRKIRVNGTKEPIEFKSNQWFGATVKAHKGKVVACAPLYHWRTLKPTPEKDPVGTCYVAIQNFSAYAEFSPCRNSNADPEGQGYCQAGFSLDFYKNGDLIVGGPGSFYWQGQVITASVADIIANYSFKDILRKLAGEKQTEVAPASYDDSYLGYSVAAGEFTGDSQQELVAGIPRGAQNFGYVSIINSTDMTFIQNFTGEQMASYFGYTVVVSDVNSDGLDDVLVGAPLFMEREFESNPREVGQIYLYLQVSSLLFRDPQILTGTETFGRFGSAMAHLGDLNQDGYNDIAIGVPFAGKDQRGKVLIYNGNKDGLNTKPSQVLQGVWASHAVPSGFGFTLRGDSDIDKNDYPDLIVGAFGTGKVAVYRARPVVTVDAQLLLHPMIINLENKTCQVPDSMTSAACFSLRVCASVTGQSIANTIVLMAEVQLDSLKQKGAIKRTLFLDNHQAHRVFPLVIKRQKSHQCQDFIVYLRDETEFRDKLSPINISLNYSLDESTFKEGLEVKPILNYYRENIVSEQAHILVDCGEDNLCVPDLKLSARPDKHQVIIGDENHLMLIINARNEGEGAYEAELFVMIPEEADYVGIERNNKGFRPLSCEYKMENVTRMVVCDLGNPMVSGTNYSLGLRFAVPRLEKTNMSINFDLQIRSSNKDNPDSNFVSLQINITAVAQVEIRGVSHPPQIVLPIHNWEPEEEPHKEEEVGPLVEHIYELHNIGPSTISDTILEVGWPFSARDEFLLYIFHIQTLGPLQCQPNPNINPQDIKPAASPEDTPELSAFLRNSTIPHLVRKRDVHVVEFHRQSPAKILNCTNIECLQISCAVGRLEGGESAVLKVRSRLWAHTFLQRKNDPYALASLVSFEVKKMPYTDQPAKLPEGSIVIKTSVIWATPNVSFSIPL&QTDENRCLKANAKSCGECIQAGPNCGWCTNSTFLQEGMPTSARCDDLEALKKKGCPPDDIENPRGSKDIKKNKNVTNRSKGTAEKLKPEDITQIQPQQLVLRLRSGEPQTFTLKFKRAEDYPIDLYYLMDLSYSMKDDLENVKSLGTDLMNEMRRITSDFRIGFGSFVEKTVMPYISTTPAKLRNPCTSEQNCTSPFSYKNVLSLTNKGEVFNELVGKQRISGNLDSPEGGFDAIMQVAVCGSLIGWRNVTRLLVFSTDAGFHFAGDGKLGGIVLPNDGQCHLENNMYTMSHYYDYPSIAHLVQKLSENNIQTIFAVTEEFQPVYKELKNLIPKSAVGTLSANSSNVIQLIIDAYNSLSSEVILENGKLSEGVTISYKSYCKNGVNGTGENGRKCSNISIGDEVQFEISITSNKCPKKDSDSFKIRPLGFTEEVEVILQYICECECQSEGIPESPKCHEGNGTFECGACRCNEGRVGRHCECSTDEVNSEDMDAYCRKENSSEICSNNGECVCGQCVCRKRDNTNEIYSGKFCECDNFNCDRSNGLICGGNGVCKCRVCECNPNYTGSACDCSLDTSTCEASNGQICNGRGICECGVCKCTDPKFQGQTCEMCQTCLGVCAEHKECVQCRAFNKGEKKDTCTQECSYFNITKVESRDKLPQPVQPDPVSHCKEKDVDDCWFYFTYSVNGNNEVMVHVVENPECPTGPD

  • Predicted Molecular Mass

    109 kDa & 80 kDa

  • Molecular Weight

    Approximately 85-140 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by Bis-Tris PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Measured by its binding ability in a functional ELISA. Immobilized Human Fibronectin, at 5 μg/mL (100 μL/well) can bind Biotinylated Human Integrin alpha 8 beta 1 protein. The ED50 for this effect is 428.7 ng/mL .

  • Integrin alpha 8 beta 1 Protein, Human (HEK293, Flag, His) Product Image
    1/16
  • Experimental Validation Results for Integrin alpha 8 beta 1 Protein, Human (HEK293, Flag, His)
  • Integrin alpha 8 beta 1 Protein, Human (HEK293, Flag, His) Product Image