MERTK Protein, Rat (HEK293, His)

MERTK Protein, a receptor tyrosine kinase, transmits signals by binding to ligands like LGALS3, TUB, TULP1, or GAS6. Governing cell survival, migration, differentiation, and efferocytosis, MERTK undergoes autophosphorylation upon ligand binding. Docking sites for downstream signaling molecules are created, initiating interactions with GRB2 or PLCG2 and phosphorylation of MAPK1, MAPK2, FAK/PTK2, or RAC1. MERTK signaling contributes to diverse processes, including macrophage clearance, platelet aggregation, cytoskeleton reorganization, and engulfment. In the retinal pigment epithelium, MERTK regulates phagocytosis, and it suppresses the innate immune response by activating STAT1 in the Toll-like receptor pathway, promoting the production of SOCS1 and SOCS3. MERTK Protein, Rat (HEK293, His) is the recombinant rat-derived MERTK protein, expressed by HEK293, with N-6*His labeled tag. The total length of MERTK Protein, Rat (HEK293, His) is 479 a.a., with molecular weight of 55.5 kDa.

MERTK Protein is a receptor tyrosine kinase that facilitates the transmission of signals from the extracellular matrix to the cytoplasm by binding to various ligands, including LGALS3, TUB, TULP1, or GAS6. This protein governs crucial physiological processes such as cell survival, migration, differentiation, and the engulfment of apoptotic cells (efferocytosis). Upon ligand binding, autophosphorylation of MERTK occurs on its intracellular domain, creating docking sites for downstream signaling molecules. Subsequently, MERTK interacts with GRB2 or PLCG2, leading to the phosphorylation of MAPK1, MAPK2, FAK/PTK2, or RAC1. Consequently, MERTK signaling contributes to diverse processes, including macrophage clearance of apoptotic cells, platelet aggregation, cytoskeleton reorganization, and engulfment. Within the retinal pigment epithelium (RPE), MERTK serves as a regulator of phagocytosis of rod outer segment fragments. Furthermore, it plays a vital role in suppressing the innate immune response triggered by Toll-like receptors (TLRs) by activating STAT1, which selectively promotes the production of suppressors of cytokine signaling SOCS1 and SOCS3.

Rat

HEK293

N-6*His

P57097 (G19-M497)

65037  [NCBI]

GGTAEKEEEIKPDQPFSGPLPGSLPADHRPFFAPHSSGDQLSPSQTGRSHPAHTATPQMTSAASNLLPPVAFKNTIGRIVLSEHKSVKFNCSINIPNVYQETAGISWWKDGKELLGAHHSITQFYPDEEGVSIIALFSITSVQRSDNGSYICKMKVNDREVVSDPIYVEVQGLPYFTKQPESVNVTRNTAFNLTCQAVGPPEPVNIFWVQNSSRVNENPERSPSVLTVAGLTETAVFSCEAHNDKGLTVSKGVQINIKVIPSPPTEVHILNSTAHSILVSWVPGFDGYSPLQNCSIQVKEADQLSNGSVMVFNTSASPHLYEVQQLQALANYSVTVSCRNEIGWSAVSPWILASTTEGAPAVAPLNITVFLNESSNNLEIRWTKPPIKRQDGELVGYRISHVWESAGTSKELSEEVSQNGSWAQVPVQMHNATCTVRIAVITKGGIGPFSEPVDVAIPEHSRVDYAPSSTPAPGNTESM

55.5 kDa

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.