Nicastrin Protein, Mouse (HEK293, hFc)
Nicastrin is an important subunit of the γ-secretase complex and plays a key role in the endoproteolytic cleavage of integral membrane proteins, including Notch receptors and amyloid-β precursor protein (APP). In the γ-secretase complex, nicastin acts as a key regulator of Notch and Wnt signaling cascades and affects downstream processes. Nicastrin Protein, Mouse (HEK293, hFc) is the recombinant mouse-derived Nicastrin protein, expressed by HEK293 , with C-hFc labeled tag.
- Species: Mouse
- Source: HEK293
-
Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Technical Parameters
-
Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
Nicastrin is an important subunit of the γ-secretase complex and plays a key role in the endoproteolytic cleavage of integral membrane proteins, including Notch receptors and amyloid-β precursor protein (APP). In the γ-secretase complex, nicastin acts as a key regulator of Notch and Wnt signaling cascades and affects downstream processes. Nicastrin Protein, Mouse (HEK293, hFc) is the recombinant mouse-derived Nicastrin protein, expressed by HEK293 , with C-hFc labeled tag.
Background
Nicastrin, an indispensable subunit of the gamma-secretase complex, takes center stage in the intricate molecular machinery responsible for the endoproteolytic cleavage of integral membrane proteins. This includes pivotal substrates like Notch receptors and the amyloid-beta precursor protein (APP). The gamma-secretase complex, in which Nicastrin plays a crucial role, serves as a linchpin in the modulation of Notch and Wnt signaling cascades, exerting regulatory influence on downstream processes. In concert with other essential components, such as a presenilin homodimer, APH1, and PEN2, Nicastrin forms the functional gamma-secretase complex. Nicastrin's binding affinity extends to the proteolytically processed C-terminal fragments of APP, further underscoring its pivotal role in orchestrating the intricacies of this proteolytic machinery. Moreover, Nicastrin's interactions with presenilin components accentuate its integral role within the gamma-secretase complex.
Technical Parameters
-
Species Mouse
-
Source HEK293
-
Tag C-hFc
-
Accession
P57716 (G28-E668)
-
Molecular Construction
-
N-term
-
Nicastrin (G28-E668)
Accession # P57716 -
hFc
-
C-term
-
-
Protein Length
Lumenal Domain
-
Synonyms
NCSTN; APH2; Nicastrin; Anterior Pharynx-Defective 2; KIAA0253; ATAG1874
-
AA Sequence
GLCGGNSVERKIYIPLNKTAPCVRLLNATHQIGCQSSISGDTGVIHVVEKEEDLKWVLTDGPNPPYMVLLEGKLFTRDVMEKLKGTTSRIAGLAVTLAKPNSTSSFSPSVQCPNDGFGIYSNSYGPEFAHCKKTLWNELGNGLAYEDFSFPIFLLEDENETKVIKQCYQDHNLGQNGSAPSFPLCAMQLFSHMHAVISTATCMRRSFIQSTFSINPEIVCDPLSDYNVWSMLKPINTSVGLEPDVRVVVAATRLDSRSFFWNVAPGAESAVASFVTQLAAAEALHKAPDVTTLSRNVMFVFFQGETFDYIGSSRMVYDMENGKFPVRLENIDSFVELGQVALRTSLDLWMHTDPMSQKNESVKNQVEDLLATLEKSGAGVPEVVLRRLAQSQALPPSSLQRFLRARNISGVVLADHSGSFHNRYYQSIYDTAENINVTYPEWQSPEEDLNFVTDTAKALANVATVLARALYELAGGTNFSSSIQADPQTVTRLLYGFLVRANNSWFQSILKHDLRSYLDDRPLQHYIAVSSPTNTTYVVQYALANLTGKATNLTREQCQDPSKVPNESKDLYEYSWVQGPWNSNRTERLPQCVRSTVRLARALSPAFELSQWSSTEYSTWAESRWKDIQARIFLIASKELE
-
Predicted Molecular Mass
98.5 kDa
-
Molecular Weight
Approximately 117 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
-
Glycosylation
Yes
-
Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Solution
<1 EU/μg, determined by LAL method.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Powered by Bioz
