OXSR1 Protein, Human (sf9, GST)

The OXSR1 protein is a key serine/threonine protein kinase in the WNK-SPAK/OSR1 cascade, regulating ion transport, hyperosmotic stress response, and blood pressure. Downstream of WNK kinase, OXSR1 phosphorylates ion cotransporters such as SLC12A1/NKCC2, SLC12A2/NKCC1, and SLC12A3/NCC, regulating their activity. OXSR1 Protein, Human (sf9, GST) is the recombinant human-derived OXSR1 protein, expressed by Sf9 insect cells , with N-GST labeled tag.

OXSR1 Protein functions as a key effector serine/threonine-protein kinase in the WNK-SPAK/OSR1 cascade, participating in diverse cellular processes such as ion transport, response to hypertonic stress, and blood pressure regulation. It recognizes and binds proteins with a RFXV motif, acting downstream of WNK kinases to phosphorylate ion cotransporters including SLC12A1/NKCC2, SLC12A2/NKCC1, SLC12A3/NCC, SLC12A5/KCC2, and SLC12A6/KCC3, thereby modulating their activity. OXSR1 mediates regulatory volume increase in response to hyperosmotic stress and regulates NaCl reabsorption in the distal nephron by phosphorylating the thiazide-sensitive Na-Cl cotransporter SLC12A3/NCC. Additionally, it serves as a regulator of angiogenesis in endothelial cells and activates inward rectifier potassium channels KCNJ2/Kir2.1 and KCNJ4/Kir2.3 downstream of WNK1, influencing their membrane localization. OXSR1 also plays a role in phosphorylating various substrates, including RELL1, RELL2, RELT, PAK1, and PLSCR1 in the presence of RELT.

Human

Sf9 insect cells

N-GST

O95747 (M1-S527)

9943  [NCBI]

Full Length

MSEDSSALPWSINRDDYELQEVIGSGATAVVQAAYCAPKKEKVAIKRINLEKCQTSMDELLKEIQAMSQCHHPNIVSYYTSFVVKDELWLVMKLLSGGSVLDIIKHIVAKGEHKSGVLDESTIATILREVLEGLEYLHKNGQIHRDVKAGNILLGEDGSVQIADFGVSAFLATGGDITRNKVRKTFVGTPCWMAPEVMEQVRGYDFKADIWSFGITAIELATGAAPYHKYPPMKVLMLTLQNDPPSLETGVQDKEMLKKYGKSFRKMISLCLQKDPEKRPTAAELLRHKFFQKAKNKEFLQEKTLQRAPTISERAKKVRRVPGSSGRLHKTEDGGWEWSDDEFDEESEEGKAAISQLRSPRVKESISNSELFPTTDPVGTLLQVPEQISAHLPQPAGQIATQPTQVSLPPTAEPAKTAQALSSGSGSQETKIPISLVLRLRNSKKELNDIRFEFTPGRDTAEGVSQELISAGLVDGRDLVIVAANLQKIVEEPQSNRSVTFKLASGVEGSDIPDDGKLIGFAQLSIS

Approximately 80 kDa,based on SDS-PAGE under reducing conditions.

≥ 85%, as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.