PGK1 Protein, Human (HEK293, His)
Based on 1 Customer Validation
The PGK1 protein plays a key role in the glycolysis pathway by catalyzing one of two ATP-generating reactions, promoting the reversible conversion of 1,3-bisphosphoglycerate to 3-phosphoglycerate. In addition to its glycolytic function, PGK1 appears to act as a cofactor protein for polymerase α, playing a role in primer recognition. PGK1 Protein, Human (HEK293, His) is the recombinant human-derived PGK1 protein, expressed by HEK293 , with C-6*His labeled tag.
- Species: Human
- Source: HEK293
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
The PGK1 protein plays a key role in the glycolysis pathway by catalyzing one of two ATP-generating reactions, promoting the reversible conversion of 1,3-bisphosphoglycerate to 3-phosphoglycerate. In addition to its glycolytic function, PGK1 appears to act as a cofactor protein for polymerase α, playing a role in primer recognition. PGK1 Protein, Human (HEK293, His) is the recombinant human-derived PGK1 protein, expressed by HEK293 , with C-6*His labeled tag.
Background
Phosphoglycerate kinase 1 (PGK1) plays a crucial role in glycolysis by catalyzing the reversible conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate, contributing to the production of ATP. Beyond its established role in glycolytic energy metabolism, PGK1 is suggested to function as a cofactor protein, particularly in association with polymerase alpha, where it acts as a primer recognition protein. Additionally, PGK1 may play a role in sperm motility, indicating potential contributions to cellular processes beyond its classic glycolytic function. The multifaceted roles attributed to PGK1 underscore its significance in cellular bioenergetics and suggest additional functions in cellular processes such as DNA replication and sperm motility.
Verified Bioactivity
Measured by NADH production in a reaction coupled with GAPDH. The specific activity is > 50000 pmol/min/μg, as measured under the described conditions.
MCE Validation Data
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Purity - SDS-PAGE
Purity - SDS-PAGE
Technical Parameters
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Species Human
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Source HEK293
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Tag C-6*His
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Accession
P00558-1 (S2-I417)
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Molecular Construction
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N-term
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PGK1 (S2-I417)
Accession # P00558 -
6*His
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C-term
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Protein Length
Full Length of Isoform-1 Mature Protein
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Synonyms
PGK1; PGKA; Phosphoglycerate Kinase 1; Epididymis Secretory Sperm Binding Protein Li 68p; Cell Migration-Inducing Gene 10 Protein; Phosphoglycerate Kinase; Primer Recognition Protein 2; HEL-S-68p; PRP 2; MIG10
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AA Sequence
SLSNKLTLDKLDVKGKRVVMRVDFNVPMKNNQITNNQRIKAAVPSIKFCLDNGAKSVVLMSHLGRPDGVPMPDKYSLEPVAVELKSLLGKDVLFLKDCVGPEVEKACANPAAGSVILLENLRFHVEEEGKGKDASGNKVKAEPAKIEAFRASLSKLGDVYVNDAFGTAHRAHSSMVGVNLPQKAGGFLMKKELNYFAKALESPERPFLAILGGAKVADKIQLINNMLDKVNEMIIGGGMAFTFLKVLNNMEIGTSLFDEEGAKIVKDLMSKAEKNGVKITLPVDFVTADKFDENAKTGQATVASGIPAGWMGLDCGPESSKKYAEAVTRAKQIVWNGPVGVFEWEAFARGTKALMDEVVKATSRGCITIIGGGDTATCCAKWNTEDKVSHVSTGGGASLELLEGKVLPGVDALSNI
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Molecular Weight
Approximately 37-50 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Solution
1.Supplied as a 0.22 μm filtered solution of 20 mM Tris-HCl, 150 mM NaCl, 20% glycerol, pH 8.0.
2.Supplied as a 0.22 μm filtered solution of 50 mM Tris, 300 mM NaCl, pH 8.0, 20% glycerol.
Please refer to the lot-specific COA for specific buffer information.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.
<1 EU/μg, determined by LAL method.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
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Data Sheet (238 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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