PLAU/uPA Protein, Mouse (HEK293, C-His-Avi)

PLAU or urokinase-type plasminogen activator (uPA) critically initiates fibrinolysis by cleaving plasminogen to form active plasmin.This enzymatic conversion is critical for regulating clot dissolution and tissue remodeling.PLAU/uPA Protein, Mouse (HEK293, C-His-Avi) is the recombinant mouse-derived PLAU/uPA protein, expressed by HEK293 , with C-His-Avi labeled tag.

PLAU, also known as urokinase-type plasminogen activator (uPA), plays a crucial role in fibrinolysis by specifically cleaving the zymogen plasminogen to generate the active enzyme plasmin. This enzymatic conversion is a pivotal step in the regulation of blood clot dissolution and tissue remodeling. PLAU's ability to activate plasmin sets in motion a cascade of proteolytic events, contributing to the breakdown of fibrin clots and extracellular matrix components. This process is essential for various physiological functions, including wound healing, tissue repair, and the resolution of blood clots.

1.Human uPAR, hFc Tag captured on CM5 Chip via Protein A can bind Mouse PLAU, His-Avi Tag with an affinity constant of 0.33-10.24 μM as determined in SPR assay (Biacore T200).
2.Measured by its ability to cleave 1 mM fluorogenic peptide substrate, N-carbobenzyloxy-Gly-Gly-Arg-7-amido-4-methylcoumarin (HY-P0019) that incubate at room temperature in kinetic mode for 5 minutes. The specific activity is >1700 pmol/min/μg.
3.Measured by its ability to cleave 2 mM fluorogenic peptide substrate, N-carbobenzyloxy-Gly-Gly-Arg-7-amido-4-methylcoumarin (HY-P0019) that incubate at room temperature in kinetic mode for 5 minutes. The specific activity is 6287.051 pmol/min/μg.

Mouse

HEK293

C-Avi;C-His

P06869 (G21-F433)

18792  [NCBI]

Full Length of Mature Protein

GSVLGAPDESNCGCQNGGVCVSYKYFSRIRRCSCPRKFQGEHCEIDASKTCYHGNGDSYRGKANTDTKGRPCLAWNAPAVLQKPYNAHRPDAISLGLGKHNYCRNPDNQKRPWCYVQIGLRQFVQECMVHDCSLSKKPSSSVDQQGFQCGQKALRPRFKIVGGEFTEVENQPWFAAIYQKNKGGSPPSFKCGGSLISPCWVASAAHCFIQLPKKENYVVYLGQSKESSYNPGEMKFEVEQLILHEYYREDSLAYHNDIALLKIRTSTGQCAQPSRSIQTICLPPRFTDAPFGSDCEITGFGKESESDYLYPKNLKMSVVKLVSHEQCMQPHYYGSEINYKMLCAADPEWKTDSCKGDSGGPLICNIEGRPTLSGIVSWGRGCAEKNKPGVYTRVSHFLDWIQSHIGEEKGLAF

Approximately 28-35 kDa, based on SDS-PAGE under reducing conditions, due to the enzyme lysis and glycosylation.

• 
  ≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder

1.Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.
2.Lyophilized from a 0.22 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 6.0, 8% trehalose.
3.Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
Please refer to the lot-specific COA for specific buffer information.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.