Copy Link Print Information

PLAU/uPA Protein, Mouse (HEK293, C-His-Avi)

1 Cited Publications
Customer Review

Based on 1 publication(s) in Google Scholar

PLAU or urokinase-type plasminogen activator (uPA) critically initiates fibrinolysis by cleaving plasminogen to form active plasmin.This enzymatic conversion is critical for regulating clot dissolution and tissue remodeling.PLAU/uPA Protein, Mouse (HEK293, C-His-Avi) is the recombinant mouse-derived PLAU/uPA protein, expressed by HEK293 , with C-His-Avi labeled tag.

For research use only. We do not sell to patients.
  • Species: Mouse
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for PLAU/uPA Protein, Mouse (HEK293, C-His-Avi)
  • PLAU/uPA Protein, Mouse (HEK293, C-His-Avi) Product Image
Size
Price
Stock
Quantity
Amount: USD 0.00
Please select quantity
or
or

* Please select Quantity before adding items.

To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download

To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
Top Publications Citing

Use of MCE Products

Biological Activity

Description

PLAU or urokinase-type plasminogen activator (uPA) critically initiates fibrinolysis by cleaving plasminogen to form active plasmin.This enzymatic conversion is critical for regulating clot dissolution and tissue remodeling.PLAU/uPA Protein, Mouse (HEK293, C-His-Avi) is the recombinant mouse-derived PLAU/uPA protein, expressed by HEK293 , with C-His-Avi labeled tag.

Background

PLAU, also known as urokinase-type plasminogen activator (uPA), plays a crucial role in fibrinolysis by specifically cleaving the zymogen plasminogen to generate the active enzyme plasmin. This enzymatic conversion is a pivotal step in the regulation of blood clot dissolution and tissue remodeling. PLAU's ability to activate plasmin sets in motion a cascade of proteolytic events, contributing to the breakdown of fibrin clots and extracellular matrix components. This process is essential for various physiological functions, including wound healing, tissue repair, and the resolution of blood clots.

Verified Bioactivity

1.Human uPAR, hFc Tag captured on CM5 Chip via Protein A can bind Mouse PLAU, His-Avi Tag with an affinity constant of 0.33-10.24 μM as determined in SPR assay (Biacore T200).
2.Measured by its ability to cleave 1 mM fluorogenic peptide substrate, N-carbobenzyloxy-Gly-Gly-Arg-7-amido-4-methylcoumarin (HY-P0019) that incubate at room temperature in kinetic mode for 5 minutes. The specific activity is >1700 pmol/min/μg.
3.Measured by its ability to cleave 2 mM fluorogenic peptide substrate, N-carbobenzyloxy-Gly-Gly-Arg-7-amido-4-methylcoumarin (HY-P0019) that incubate at room temperature in kinetic mode for 5 minutes. The specific activity is 3000-7000 pmol/min/μg.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

Technical Parameters

  • Species Mouse
  • Source HEK293
  • Tag C-Avi;C-His
  • Accession

    P06869 (G21-F433)

  • Gene ID
    18792  [NCBI]
  • Molecular Construction
    • N-term
    • PLAU (G21-F433)
      Accession # P06869
    • His-Avi
    • C-term
  • Protein Length

    Full Length of Mature Protein

  • Synonyms

    PLAU; Plasminogen Activator, Urinary; Plasminogen Activator, Urokinase; PLAU Protein; UPA; BDPLT5; Urokinase-Type Plasminogen Activator; U-PA; URK; ATF; U-Plasminogen Activator; QPD

  • AA Sequence

    GSVLGAPDESNCGCQNGGVCVSYKYFSRIRRCSCPRKFQGEHCEIDASKTCYHGNGDSYRGKANTDTKGRPCLAWNAPAVLQKPYNAHRPDAISLGLGKHNYCRNPDNQKRPWCYVQIGLRQFVQECMVHDCSLSKKPSSSVDQQGFQCGQKALRPRFKIVGGEFTEVENQPWFAAIYQKNKGGSPPSFKCGGSLISPCWVASAAHCFIQLPKKENYVVYLGQSKESSYNPGEMKFEVEQLILHEYYREDSLAYHNDIALLKIRTSTGQCAQPSRSIQTICLPPRFTDAPFGSDCEITGFGKESESDYLYPKNLKMSVVKLVSHEQCMQPHYYGSEINYKMLCAADPEWKTDSCKGDSGGPLICNIEGRPTLSGIVSWGRGCAEKNKPGVYTRVSHFLDWIQSHIGEEKGLAF

  • Predicted Molecular Mass

    49 kDa

  • Molecular Weight

    Approximately 28-35 kDa, based on SDS-PAGE under reducing conditions, due to the enzyme lysis and glycosylation.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE or Bis-Tris PAGE.

Product Properties

Appearance

Lyophilized powder

Formulation

1.Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.
2.Lyophilized from a 0.22 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 6.0, 8% trehalose.
3.Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
Please refer to the lot-specific COA for specific buffer information.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) Volume (to add to vial)
=
Mass (in vial) Mass (in vial)
÷
Desired Reconstitution Concentration Desired Reconstitution Concentration
Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

Concentration (start) Concentration (start)
×
Volume (start) Volume (start)
=
Concentration (final) Concentration (final)
×
Volume (final) Volume (final)
The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

Get Quote In-stock

Other size
Get Quote
Please select quantity
Amount: USD 0.00

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • PLAU/uPA Protein, Mouse (HEK293, C-His-Avi) Product Image
    1/16
  • Experimental Validation Results for PLAU/uPA Protein, Mouse (HEK293, C-His-Avi)
  • PLAU/uPA Protein, Mouse (HEK293, C-His-Avi) Product Image