PPM1A Protein, Mouse (His)
Based on 1 publication(s) in Google Scholar
PPM1A is a multifunctional enzyme whose enzymatic activity exhibits broad specificity. It acts as a negative regulator of TGF-β signaling by dephosphorylating SMAD2 and SMAD3, leading to their dissociation from SMAD4, nuclear export of SMAD, and termination of TGF-β-mediated signaling. PPM1A Protein, Mouse (His) is the recombinant mouse-derived PPM1A protein, expressed by E. coli , with C-His labeled tag.
- Species: Mouse
- Source: E. coli
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
PPM1A is a multifunctional enzyme whose enzymatic activity exhibits broad specificity. It acts as a negative regulator of TGF-β signaling by dephosphorylating SMAD2 and SMAD3, leading to their dissociation from SMAD4, nuclear export of SMAD, and termination of TGF-β-mediated signaling. PPM1A Protein, Mouse (His) is the recombinant mouse-derived PPM1A protein, expressed by E. coli , with C-His labeled tag.
Protein Phosphatase, Mg2+/Mn2+ Dependent 1A (PPM1A) is an enzyme characterized by its broad specificity. It functions as a negative regulator of TGF-beta signaling by dephosphorylating SMAD2 and SMAD3, leading to their dissociation from SMAD4 and subsequent nuclear export. This enzymatic activity ultimately terminates the TGF-beta-mediated signaling pathway. Additionally, PPM1A dephosphorylates PRKAA1 and PRKAA2, contributing to the regulation of cellular processes associated with AMP-activated protein kinase. Furthermore, PPM1A plays a crucial role in the termination of TNF-alpha-mediated NF-kappa-B activation by dephosphorylating and inactivating IKBKB/IKKB. The diverse substrate specificity of PPM1A underscores its importance in modulating key signaling pathways, highlighting its regulatory functions in cellular responses to TGF-beta and TNF-alpha signaling (
Measured by its ability to dephosphorylate the peptide substrate, DLDVPIPGRFDRRVS(PO3)VAAE. The specific activity is 450.99 nmol/min/mg, as measured under the described conditions.
Publications (1)
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Journal Impact Factor
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Most Recent
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Cancer Res
SAICAR Drives T Regulatory Cell Differentiation and FOXP3 Maintenance to Promote Immunotherapy Resistance. [Abstract]2026 May 4;86(9):2218-2236. PMID: 41671386
Technical Parameters
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Species Mouse
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Source E. coli
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Tag C-6*His
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Accession
P49443/NP_032936.1 (M1-W382)
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Molecular Construction
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N-term
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PPM1A (M1-W382)
Accession # P49443/NP_032936.1 -
His
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C-term
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Protein Length
Full Length
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Synonyms
PPM1A; Protein Phosphatase 1A (Formerly 2C), Magnesium-Dependent, Alpha Isoform; Protein Phosphatase, Mg2+/Mn2+ Dependent 1A; Protein Phosphatase, Mg2+/Mn2+ Dependent, 1A; Protein Phosphatase 1A; Protein Phosphatase 2C, Alpha Isoform; PP2Calpha; Alternati
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AA Sequence
MGAFLDKPKMEKHNAQGQGNGLRYGLSSMQGWRVEMEDAHTAVIGLPSGLETWSFFAVYDGHAGSQVAKYCCEHLLDHITNNQDFRGSAGAPSVENVKNGIRTGFLEIDEHMRVMSEKKHGADRSGSTAVGVLISPQHTYFINCGDSRGLLCRNRKVHFFTQDHKPSNPLEKERIQNAGGSVMIQRVNGSLAVSRALGDFDYKCVHGKGPTEQLVSPEPEVHDIERSEEDDQFIILACDGIWDVMGNEELCDFVRSRLEVTDDLEKVCNEVVDTCLYKGSRDNMSVILICFPSAPKVSAEAVKKEAELDKYLESRVEEIIKKQVEGVPDLVHVMRTLASENIPSLPPGGELASKRNVIEAVYNRLNPYKNDDTDSASTDDMW
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Molecular Weight
Approximately 44 kDa
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (237 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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