PPM1A Protein, Mouse (His)

PPM1A is a multifunctional enzyme whose enzymatic activity exhibits broad specificity. It acts as a negative regulator of TGF-β signaling by dephosphorylating SMAD2 and SMAD3, leading to their dissociation from SMAD4, nuclear export of SMAD, and termination of TGF-β-mediated signaling. PPM1A Protein, Mouse (His) is the recombinant mouse-derived PPM1A protein, expressed by E. coli , with C-His labeled tag. The total length of PPM1A Protein, Mouse (His) is 382 a.a., with molecular weight of ~44 kDa.

Protein Phosphatase, Mg2+/Mn2+ Dependent 1A (PPM1A) is an enzyme characterized by its broad specificity. It functions as a negative regulator of TGF-beta signaling by dephosphorylating SMAD2 and SMAD3, leading to their dissociation from SMAD4 and subsequent nuclear export. This enzymatic activity ultimately terminates the TGF-beta-mediated signaling pathway. Additionally, PPM1A dephosphorylates PRKAA1 and PRKAA2, contributing to the regulation of cellular processes associated with AMP-activated protein kinase. Furthermore, PPM1A plays a crucial role in the termination of TNF-alpha-mediated NF-kappa-B activation by dephosphorylating and inactivating IKBKB/IKKB. The diverse substrate specificity of PPM1A underscores its importance in modulating key signaling pathways, highlighting its regulatory functions in cellular responses to TGF-beta and TNF-alpha signaling (

Measured by its ability to dephosphorylate the peptide substrate, DLDVPIPGRFDRRVS(PO3)VAAE. The specific activity is 450.99 nmol/min/mg, as measured under the described conditions.

Mouse

E. coli

C-6*His

P49443 (M1-W382)

19042  [NCBI]

MGAFLDKPKMEKHNAQGQGNGLRYGLSSMQGWRVEMEDAHTAVIGLPSGLETWSFFAVYDGHAGSQVAKYCCEHLLDHITNNQDFRGSAGAPSVENVKNGIRTGFLEIDEHMRVMSEKKHGADRSGSTAVGVLISPQHTYFINCGDSRGLLCRNRKVHFFTQDHKPSNPLEKERIQNAGGSVMIQRVNGSLAVSRALGDFDYKCVHGKGPTEQLVSPEPEVHDIERSEEDDQFIILACDGIWDVMGNEELCDFVRSRLEVTDDLEKVCNEVVDTCLYKGSRDNMSVILICFPSAPKVSAEAVKKEAELDKYLESRVEEIIKKQVEGVPDLVHVMRTLASENIPSLPPGGELASKRNVIEAVYNRLNPYKNDDTDSASTDDMW

Approximately 44 kDa

Greater than 95% as determined by reducing SDS-PAGE

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.