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PRKAR1A Protein, Human (HEK293, His)

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PRKAR1A protein is a regulatory subunit of cAMP-dependent protein kinase and is critical for cAMP signaling. Upon activation by cAMP, it releases two catalytic monomers and a regulatory dimer from the inactive holoenzyme. PRKAR1A Protein, Human (HEK293, His) is the recombinant human-derived PRKAR1A protein, expressed by HEK293 , with C-6*His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • PRKAR1A Protein, Human (HEK293, His) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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[email protected] [email protected]
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  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
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Biological Activity

Description

PRKAR1A protein is a regulatory subunit of cAMP-dependent protein kinase and is critical for cAMP signaling. Upon activation by cAMP, it releases two catalytic monomers and a regulatory dimer from the inactive holoenzyme. PRKAR1A Protein, Human (HEK293, His) is the recombinant human-derived PRKAR1A protein, expressed by HEK293 , with C-6*His labeled tag.

Background

PRKAR1A Protein, a regulatory subunit of cAMP-dependent protein kinases, plays a crucial role in cAMP signaling within cells. The inactive holoenzyme consists of two regulatory chains and two catalytic chains, which are released upon activation by cAMP, resulting in the formation of two active catalytic monomers and a regulatory dimer. PRKAR1A Protein interacts with PRKACA and PRKACB, and this interaction has been shown to influence various cellular processes. Additionally, PRKAR1A Protein engages with RFC2, potentially contributing to cell survival. It also interacts with AKAP4, RARA (in the presence of cAMP or FSH, regulating RARA transcriptional activity), phosphorylated PJA2, CBFA2T3 (By similarity), PRKX (regulating this cAMP-dependent protein kinase), C2orf88/smAKAP (possibly targeting PRKAR1A to the plasma membrane), and AICDA, further highlighting its involvement in diverse molecular interactions and pathways.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag C-6*His
  • Accession

    P10644 (E2-V381)

  • Gene ID
    5573  [NCBI]
  • Molecular Construction
    • N-term
    • PRKAR1A (E2-V381)
      Accession # P10644
    • 6*His
    • C-term
  • Protein Length

    Full Length

  • Synonyms

    PRKAR1A; Carney Complex Type 1; Prev. PRKAR1; CAMP-Dependent Protein Kinase Type I-Alpha Regulatory Chain; Prev. PKR1; CAMP-Dependent Protein Kinase Regulatory Subunit RIalpha; Prev. TSE1; Epididymis Secretory Sperm Binding Protein; Prev. Prkar1alpha; Tis

  • AA Sequence

    ESGSTAASEEARSLRECELYVQKHNIQALLKDSIVQLCTARPERPMAFLREYFERLEKEEAKQIQNLQKAGTRTDSREDEISPPPPNPVVKGRRRRGAISAEVYTEEDAASYVRKVIPKDYKTMAALAKAIEKNVLFSHLDDNERSDIFDAMFSVSFIAGETVIQQGDEGDNFYVIDQGETDVYVNNEWATSVGEGGSFGELALIYGTPRAATVKAKTNVKLWGIDRDSYRRILMGSTLRKRKMYEEFLSKVSILESLDKWERLTVADALEPVQFEDGQKIVVQGEPGDEFFIILEGSAAVLQRRSENEEFVEVGRLGPSDYFGEIALLMNRPRAATVVARGPLKCVKLDRPRFERVLGPCSDILKRNIQQYNSFVSLSV

  • Predicted Molecular Mass

    44 kDa

  • Molecular Weight

    Approximately 45-55 kDa, based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 90%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

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