PRMT6 Protein, Human (HEK293, His-Flag)

PRMT6 is a multifunctional arginine methyltransferase that prefers the formation of asymmetric dimethylarginine (aDMA) and methylates arginine residues within glycine- and arginine-rich domains change. It coordinates asymmetric dimethylation of histone H3 "Arg-2" to generate H3R2me2a, a unique epigenetic tag associated with transcriptional repression. PRMT6 Protein, Human (HEK293, His-Flag) is the recombinant human-derived PRMT6 protein, expressed by HEK293 , with N-Flag, C-His labeled tag.

PRMT6, a versatile arginine methyltransferase, demonstrates the capability to catalyze the formation of omega-N monomethylarginine (MMA) and asymmetrical dimethylarginine (aDMA), exhibiting a strong preference for aDMA formation. It predominantly methylates arginyl residues within a glycine and arginine-rich domain and shows a bias toward monomethylated substrates. Notably, PRMT6 orchestrates the asymmetric dimethylation of histone H3 'Arg-2,' leading to the creation of H3R2me2a, a distinctive epigenetic tag associated with transcriptional repression. This modification is mutually exclusive with methylation on histone H3 'Lys-4' (H3K4me2 and H3K4me3). PRMT6 serves as a transcriptional repressor for various genes, including HOXA2, THBS1, and TP53, where repression of TP53 impedes cellular senescence. Additionally, PRMT6 methylates histone H2A and H4 'Arg-3' (H2AR3me and H4R3me) and acts as a regulator of DNA base excision during repair by methylating DNA polymerase beta (POLB), enhancing its polymerase activity. It functions as a transcriptional coactivator for steroid hormone receptors, participating in gluconeogenic program activation and regulating alternative splicing events. PRMT6 also plays a role in innate immunity against HIV-1 by methylating and impairing the function of various HIV-1 proteins. Furthermore, it methylates GPS2, providing protection from ubiquitination and degradation, and targets SIRT7, inhibiting its histone deacetylase activity and promoting mitochondria biogenesis.

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

HEK293

N-Flag;C-His

Q96LA8-1/NP_060607.2 (M1-D375)

55170  [NCBI]

Full Length of Isoform-1

MSQPKKRKLESGGGGEGGEGTEEEDGAEREAALERPRRTKRERDQLYYECYSDVSVHEEMIADRVRTDAYRLGILRNWAALRGKTVLDVGAGTGILSIFCAQAGARRVYAVEASAIWQQAREVVRFNGLEDRVHVLPGPVETVELPEQVDAIVSEWMGYGLLHESMLSSVLHARTKWLKEGGLLLPASAELFIAPISDQMLEWRLGFWSQVKQHYGVDMSCLEGFATRCLMGHSEIVVQGLSGEDVLARPQRFAQLELSRAGLEQELEAGVGGRFRCSCYGSAPMHGFAIWFQVTFPGGESEKPLVLSTSPFHPATHWKQALLYLNEPVQVEQDTDVSGEITLLPSRDNPRRLRVLLRYKVGDQEEKTKDFAMED

Approximately 43-46,based on SDS-PAGE under reducing conditions.

≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4, 5% Trehalose, 5% Mannitol, 0.01% Tween-80.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.