2. Recombinant Proteins
  3. CD Antigens
  4. NK Cell CD Proteins
  5. CD100/Semaphorin-4D
  6. Semaphorin-4D/SEMA4D Protein, Mouse (HEK293, His)

Semaphorin-4D/SEMA4D Protein, Mouse (HEK293, His)

Art. -Nr.: HY-P74563
COA
SDS
Handling Instructions Technical Support

Semaphorin-4D/SEMA4D is the cell surface receptor for PLXNB1 and PLXNB2 and plays a crucial role in intercellular signaling.It regulates GABAergic synapse development and promotes inhibitory synapses in a PLXNB1-dependent manner.Semaphorin-4D/SEMA4D Protein, Mouse (HEK293, His) is the recombinant mouse-derived Semaphorin-4D/SEMA4D protein, expressed by HEK293 , with C-His labeled tag.

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Beschreibung

Semaphorin-4D/SEMA4D is the cell surface receptor for PLXNB1 and PLXNB2 and plays a crucial role in intercellular signaling.It regulates GABAergic synapse development and promotes inhibitory synapses in a PLXNB1-dependent manner.Semaphorin-4D/SEMA4D Protein, Mouse (HEK293, His) is the recombinant mouse-derived Semaphorin-4D/SEMA4D protein, expressed by HEK293 , with C-His labeled tag.
Background

Semaphorin-4D (SEMA4D) operates as a cell surface receptor for PLXNB1 and PLXNB2, assuming a crucial role in cell-cell signaling. This versatile protein is implicated in diverse cellular processes, including the regulation of GABAergic synapse development, where it fosters the development of inhibitory synapses in a PLXNB1-dependent manner. In hippocampal neurons, SEMA4D influences the complexity and arborization of developing neurites by activating PLXNB1, thereby mediating RHOA activation. Additionally, SEMA4D is involved in promoting the migration of cerebellar granule cells and induces B-cell aggregation, enhancing their viability in vitro. Furthermore, SEMA4D stimulates endothelial cell migration through the activation of PTK2B/PYK2, SRC, and the phosphatidylinositol 3-kinase-AKT pathway. Operating as a homodimer, SEMA4D interacts with both PLXNB1 and PLXNB2, underscoring its intricate involvement in various cellular and developmental processes.
Species

Mouse
Source

HEK293
Tag

C-His
Accession

O09126 (M1-R733)
Gene ID
Molecular Construction
N-term
SEMA4D (M1-R733)
Accession # O09126
His
C-term
Protein Length

Partial
Synonyms
SEMA4D; Semaphorin-4D; M-Sema G; Semaphorin-C-like 2; Sema J; CD100; Semacl2
AA Sequence

MRMCAPVRGLFLALVVVLRTAVAFAPVPRLTWEHGEVGLVQFHKPGIFNYSALLMSEDKDTLYVGAREAVFAVNALNISEKQHEVYWKVSEDKKSKCAEKGKSKQTECLNYIRVLQPLSSTSLYVCGTNAFQPTCDHLNLTSFKFLGKSEDGKGRCPFDPAHSYTSVMVGGELYSGTSYNFLGSEPIISRNSSHSPLRTEYAIPWLNEPSFVFADVIQKSPDGPEGEDDKVYFFFTEVSVEYEFVFKLMIPRVARVCKGDQGGLRTLQKKWTSFLKARLICSKPDSGLVFNILQDVFVLRAPGLKEPVFYAVFTPQLNNVGLSAVCAYTLATVEAVFSRGKYMQSATVEQSHTKWVRYNGPVPTPRPGACIDSEARAANYTSSLNLPDKTLQFVKDHPLMDDSVTPIDNRPKLIKKDVNYTQIVVDRTQALDGTFYDVMFISTDRGALHKAVILTKEVHVIEETQLFRDSEPVLTLLLSSKKGRKFVYAGSNSGVVQAPLAFCEKHGSCEDCVLARDPYCAWSPAIKACVTLHQEEASSRGWIQDMSGDTSSCLDKSKESFNQHFFKHGGTAELKCFQKSNLARVVWKFQNGELKAASPKYGFVGRKHLLIFNLSDGDSGVYQCLSEERVRNKTVSQLLAKHVLEVKMVPRTPPSPTSEDAQTEGSKITSKMPVASTQGSSPPTPALWATSPRAATLPPKSSSGTSCEPKMVINTVPQLHSEKTVYLKSSDNR

Predicted Molecular Mass
80.2 kDa
Molekulargewicht

Approximately 100-110 kDa,based on SDS-PAGE under reducing conditions,due to the glycosylation.
Glycosylation
Yes
Reinheit

≥ 95%, as determined by reducing SDS-PAGE.
Endotoxin Level

<1 EU/μg, determined by LAL method.
Dokumentation
SDS (251 KB)

COA (220 KB)

Handling Instructions (2659 KB)

Semaphorin-4D/SEMA4D Protein, Mouse (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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