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Semaphorin-4D/SEMA4D Protein, Mouse (HEK293, His)

Semaphorin-4D/SEMA4D is the cell surface receptor for PLXNB1 and PLXNB2 and plays a crucial role in intercellular signaling.It regulates GABAergic synapse development and promotes inhibitory synapses in a PLXNB1-dependent manner.Semaphorin-4D/SEMA4D Protein, Mouse (HEK293, His) is the recombinant mouse-derived Semaphorin-4D/SEMA4D protein, expressed by HEK293 , with C-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Mouse
  • Source: HEK293
  • Storage:
    Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
  • Semaphorin-4D/SEMA4D Protein, Mouse (HEK293, His) Product Image
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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
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  • Biological Activity
  • Technical Parameters
  • Product Properties
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Biological Activity

Description

Semaphorin-4D/SEMA4D is the cell surface receptor for PLXNB1 and PLXNB2 and plays a crucial role in intercellular signaling.It regulates GABAergic synapse development and promotes inhibitory synapses in a PLXNB1-dependent manner.Semaphorin-4D/SEMA4D Protein, Mouse (HEK293, His) is the recombinant mouse-derived Semaphorin-4D/SEMA4D protein, expressed by HEK293 , with C-His labeled tag.

Background

Semaphorin-4D (SEMA4D) operates as a cell surface receptor for PLXNB1 and PLXNB2, assuming a crucial role in cell-cell signaling. This versatile protein is implicated in diverse cellular processes, including the regulation of GABAergic synapse development, where it fosters the development of inhibitory synapses in a PLXNB1-dependent manner. In hippocampal neurons, SEMA4D influences the complexity and arborization of developing neurites by activating PLXNB1, thereby mediating RHOA activation. Additionally, SEMA4D is involved in promoting the migration of cerebellar granule cells and induces B-cell aggregation, enhancing their viability in vitro. Furthermore, SEMA4D stimulates endothelial cell migration through the activation of PTK2B/PYK2, SRC, and the phosphatidylinositol 3-kinase-AKT pathway. Operating as a homodimer, SEMA4D interacts with both PLXNB1 and PLXNB2, underscoring its intricate involvement in various cellular and developmental processes.

Technical Parameters

  • Species Mouse
  • Source HEK293
  • Tag C-His
  • Accession

    O09126 (M1-R733)

  • Gene ID
    20354  [NCBI]
  • Molecular Construction
    • N-term
    • SEMA4D (M1-R733)
      Accession # O09126
    • His
    • C-term
  • Protein Length

    Partial Extracellular Domain

  • Synonyms

    SEMA4D; BB18; Prev. C9orf164; A8; Prev. SEMAJ; Sema Domain, Immunoglobulin Domain (Ig), Transmembrane Domain (TM) And Short Cytoplasmic Domain, 4D; CD100; Chromosome 9 Open Reading Frame 164; Semaphorin-4D; CD100 Antigen; Coll-4; M-Sema G; Sema Domain, Im

  • AA Sequence

    MRMCAPVRGLFLALVVVLRTAVAFAPVPRLTWEHGEVGLVQFHKPGIFNYSALLMSEDKDTLYVGAREAVFAVNALNISEKQHEVYWKVSEDKKSKCAEKGKSKQTECLNYIRVLQPLSSTSLYVCGTNAFQPTCDHLNLTSFKFLGKSEDGKGRCPFDPAHSYTSVMVGGELYSGTSYNFLGSEPIISRNSSHSPLRTEYAIPWLNEPSFVFADVIQKSPDGPEGEDDKVYFFFTEVSVEYEFVFKLMIPRVARVCKGDQGGLRTLQKKWTSFLKARLICSKPDSGLVFNILQDVFVLRAPGLKEPVFYAVFTPQLNNVGLSAVCAYTLATVEAVFSRGKYMQSATVEQSHTKWVRYNGPVPTPRPGACIDSEARAANYTSSLNLPDKTLQFVKDHPLMDDSVTPIDNRPKLIKKDVNYTQIVVDRTQALDGTFYDVMFISTDRGALHKAVILTKEVHVIEETQLFRDSEPVLTLLLSSKKGRKFVYAGSNSGVVQAPLAFCEKHGSCEDCVLARDPYCAWSPAIKACVTLHQEEASSRGWIQDMSGDTSSCLDKSKESFNQHFFKHGGTAELKCFQKSNLARVVWKFQNGELKAASPKYGFVGRKHLLIFNLSDGDSGVYQCLSEERVRNKTVSQLLAKHVLEVKMVPRTPPSPTSEDAQTEGSKITSKMPVASTQGSSPPTPALWATSPRAATLPPKSSSGTSCEPKMVINTVPQLHSEKTVYLKSSDNR

  • Predicted Molecular Mass

    80.2 kDa

  • Molecular Weight

    Approximately 100-110 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Solution

Endotoxin Level

<1 EU/μg, determined by LAL method.

Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Calculators

Reconstitution Calculator

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Dilution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
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