SHMT1 Protein, Human (HEK293, His)
Based on 1 Customer Validation
SHMT1 Protein, Human (HEK293, His) is the recombinant human-derived SHMT1 protein, expressed by HEK293, with C-6*His labeled tag.
- Species: Human
- Source: HEK293
-
Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Technical Parameters
-
Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
SHMT1 Protein, Human (HEK293, His) is the recombinant human-derived SHMT1 protein, expressed by HEK293, with C-6*His labeled tag.
Background
SHMT1 is a pyridoxal phosphate-containing enzyme that catalyzes the reversible conversion of serine and tetrahydrofolate to glycine and 5,10-methylenetetrahydrofolate. This reaction provides a carbon unit for the synthesis of methionine, thymidylate, and purine in the cytoplasm. SHMT1 promotes low-grade glioma progression by activating mTORC1 signaling. Targeting SHMT1 and SHMT2 can treat T-cell acute lymphoblastic leukemia. SHMT1 hypermethylation is significantly associated with elevated homocysteine (Hcy) concentrations in ischemic stroke and can be used as a potential indicator for ischemic stroke in women.
Technical Parameters
-
Species Human
-
Source HEK293
-
Tag C-6*His
-
Accession
AAH07979.1 (M3-F483)
-
Molecular Construction
-
N-term
-
SHMT1 (M3-F483)
Accession # AAH07979.1 -
6*His
-
C-term
-
-
Protein Length
Full Length
-
Synonyms
SHMT1; Serine Hydroxymethyltransferase 1 (Soluble); Serine Hydroxymethyltransferase 1; L-Allo-Threonine/L-Threonine Aldolase SHMT1; CSHMT; Serine Hydroxymethyltransferase, Cytosolic; SHMT; MGC15229; Glycine Hydroxymethyltransferase; MGC24556; Serine Methylase; HcSHMT; Cytoplasmic Serine Hydroxymethyltransferase; 14 KDa Protein
-
AA Sequence
MPVNGAHKDADLWSSHDKMLAQPLKDSDVEVYNIIKKESNRQRVGLELIASENFASRAVLEALGSCLNNKYSEGYPGQRYYGGTEFIDELETLCQKRALQAYKLDPQCWGVNVQPYSGSPANFAVYTALVEPHGRIMGLDLPDGGHLTHGFMTDKKKISATSIFFESMPYKVNPDTGYINYDQLEENARLFHPKLIIAGTSCYSRNLEYARLRKIADENGAYLMADMAHISGLVAAGVVPSPFEHCHVVTTTTHKTLRGCRAGMIFYRKGVKSVDPKTGKEILYNLESLINSAVFPGLQGGPHNHAIAGVAVALKQAMTLEFKVYQHQVVANCRALSEALTELGYKIVTGGSDNHLILVDLRSKGTDGGRAEKVLEACSIACNKNTCPGDRSALRPSGLRLGTPALTSRGLLEKDFQKVAHFIHRGIELTLQIQSDTGVRATLKEFKERLAGDKYQAAVQALREEVESFASFFPLPGLPDF
-
Predicted Molecular Mass
53.9 kDa
-
Molecular Weight
Approximately 55 kDa, based on SDS-PAGE under reducing conditions.
-
Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
1.Lyophilized from a 0.22 μm filtered solution of 20 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, 5% trehalose, pH 8.0.
2.Lyophilized from a 0.22 μm filtered solution of 20 mM PB, 150 mM NaCl, 1 mM EDTA, 5% trehalose, 5% mannitol, 0.02% Tween 80, pH 6.0.
Please refer to the lot-specific COA for specific buffer information.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
-
Data Sheet (238 KB)
-
SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
-
Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Powered by Bioz
