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  4. Src Protein, Human (sf9, His-GST)

Src Protein, Human (sf9, His-GST)

Cat. No.: HY-P73422
COA Handling Instructions

Src is a non-receptor protein tyrosine kinase that centralizes multiple cellular processes triggered by various receptors. It controls gene transcription, immune response, adhesion, cell cycle, apoptosis, migration and transformation. Src Protein, Human (sf9, His-GST) is the recombinant human-derived Src protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag. The total length of Src Protein, Human (sf9, His-GST) is 536 a.a., with molecular weight of ~81 kDa.

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Description

Src is a non-receptor protein tyrosine kinase that centralizes multiple cellular processes triggered by various receptors. It controls gene transcription, immune response, adhesion, cell cycle, apoptosis, migration and transformation. Src Protein, Human (sf9, His-GST) is the recombinant human-derived Src protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag. The total length of Src Protein, Human (sf9, His-GST) is 536 a.a., with molecular weight of ~81 kDa.

Background

Src, a non-receptor protein tyrosine kinase, emerges as a central player in an array of cellular processes triggered by the engagement of diverse receptors, including immune response receptors, integrins, receptor protein tyrosine kinases, G protein-coupled receptors, and cytokine receptors. It orchestrates signaling pathways governing gene transcription, immune responses, cell adhesion, cell cycle progression, apoptosis, migration, and cellular transformation. While functional redundancy within the SRC kinase family complicates the identification of specific roles, SRC appears to be a primary kinase activated upon receptor engagement, influencing the activation of other protein tyrosine kinase (PTK) families. SRC is implicated in the regulation of cytoskeletal organization by phosphorylating substrates like AFAP1 and cortactin (CTTN). Its involvement extends to cell-cell and cell-matrix junctions, where it phosphorylates components such as beta-catenin (CTNNB1), delta-catenin (CTNND1), plakoglobin (JUP), and connexin-43 (GJA1). Additionally, SRC participates in PDGF-mediated tyrosine phosphorylation of STAT1 and STAT3, the RAS pathway, EGF-mediated calcium-activated chloride channel activation, and beta-arrestin desensitization. It also plays a crucial role in EGFR internalization, CDK20/MAPK3 mitogen-activated protein kinase cascade stimulation, and osteoclastic bone resorption. SRC's multifaceted activities highlight its intricate role in cellular regulation and signal transduction.

Biological Activity

The specific activity was determined to be >80 nmol/min/mg using Poly (Glu:Tyr) 4:1 as substrate.

Species

Human

Source

Sf9 insect cells

Tag

N-His;N-GST

Accession

P12931 (M1-L536)

Gene ID
Molecular Construction
N-term
His-GST
Src (M1-L536)
Accession # P12931
C-term
Synonyms
Proto-oncogene tyrosine-protein kinase Src; Src; p60-Src; SRC1
Molecular Weight

Approximately 81 kDa

Purity

Greater than 90% as determined by reducing SDS-PAGE

Appearance

Solution.

Formulation

Supplied as sterile 50 mM Tris, 100 mM NaCl, pH 8.0, 20% glycerol, 0.3 mM DTT

Endotoxin Level

<1 EU/μg, determined by LAL method.

Storage & Stability

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Documentation

Src Protein, Human (sf9, His-GST) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Src Protein, Human (sf9, His-GST)
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HY-P73422
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