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  3. TRAC Protein, Human (P.pastoris, His)

TRAC Protein, Human (P.pastoris, His)

Cat. No.: HY-P72271
COA Handling Instructions

The TRAC protein is part of the T-cell receptor and is critical for immune responses. TRAC Protein, Human (P.pastoris, His) is the recombinant human-derived TRAC protein, expressed by P. pastoris , with N-6*His labeled tag. The total length of TRAC Protein, Human (P.pastoris, His) is 140 a.a., with molecular weight of ~17.0 kDa.

For research use only. We do not sell to patients.

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Description

The TRAC protein is part of the T-cell receptor and is critical for immune responses. TRAC Protein, Human (P.pastoris, His) is the recombinant human-derived TRAC protein, expressed by P. pastoris , with N-6*His labeled tag. The total length of TRAC Protein, Human (P.pastoris, His) is 140 a.a., with molecular weight of ~17.0 kDa.

Background

The constant region of the T cell receptor (TR) alpha chain, represented by the TRAC protein, is a fundamental component of alpha-beta T cell receptors crucial for the immune response. These antigen-specific receptors are present on the surface of T lymphocytes and play a pivotal role in recognizing peptide-major histocompatibility (pMH) complexes displayed by antigen-presenting cells (APC), essential for effective T cell adaptive immunity against pathogens. Binding of the alpha-beta TR to the pMH complex initiates TR-CD3 clustering on the cell surface, activating intracellular LCK that phosphorylates the ITAM motifs of CD3G, CD3D, CD3E, and CD247, facilitating the recruitment of ZAP70. ZAP70, in turn, phosphorylates LAT, assembling the LAT signalosome, which branches signals to the calcium, mitogen-activated protein kinase (MAPK), and nuclear factor NF-kappa-B (NF-kB) pathways. This leads to the mobilization of transcription factors critical for gene expression and essential for T cell growth and differentiation. The T cell repertoire, generated in the thymus through V-(D)-J rearrangement, undergoes intrathymic selection to form a self-major histocompatibility (MH) restricted, non-autoaggressive peripheral T cell pool. Post-thymic interactions with pMH complexes shape the structural and functional avidity of the alpha-beta TR, a heterodimer composed of an alpha and beta chain linked by disulfide bonds. The assembly of alpha-beta TR heterodimers with CD3 coreceptor proteins occurs in the endoplasmic reticulum, forming the TR-CD3 (TcR or TCR). This assembly involves the association of TR alpha and TR beta chains with CD3D-CD3E and CD3G-CD3E heterodimers, respectively, and the final association with the CD247 homodimer, a critical step for transport to the cell surface.

Species

Human

Source

P. pastoris

Tag

N-6*His

Accession

P01848 (I1-S140)

Gene ID
Molecular Construction
N-term
6*His
TRAC (I1-S140)
Accession # P01848
C-term
Synonyms
TRAC; TCRA; T cell receptor alpha constant
AA Sequence

IQNPDPAVYQLRDSKSSDKSVCLFTDFDSQTNVSQSKDSDVYITDKTVLDMRSMDFKSNSAVAWSNKSDFACANAFNNSIIPEDTFFPSPESSCDVKLVEKSFETDTNLNFQNLSVIGFRILLLKVAGFNLLMTLRLWSS

Molecular Weight

Approximately 17.0 kDa

Purity
  • Greater than 98% as determined by reducing SDS-PAGE.
Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.2 μm sterile filtered 20 mM Tris-HCl, 0.5 M NaCl, 3% Trehalose, pH 8.0.

Endotoxin Level

<1.0 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

TRAC Protein, Human (P.pastoris, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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TRAC Protein, Human (P.pastoris, His)
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