1. Recombinant Proteins
  2. CD Antigens
  3. Macrophage CD Proteins Stem Cell CD Proteins Endothelial cell CD Proteins
  4. Thy-1/CD90
  5. Thy1/CD90 Protein, Mouse (HEK293, His)

Thy1/CD90 protein may play a role in cell-cell interactions, especially during synaptogenesis in the brain. It facilitates communication and connections between cells, shaping neural network architecture and functionality. Understanding how Thy1/CD90 participates in these interactions can provide insights into its role in brain function and connectivity. Thy1/CD90 Protein, Mouse (HEK293, His) is the recombinant mouse-derived Thy1/CD90 protein, expressed by HEK293 , with C-His labeled tag.

For research use only. We do not sell to patients.

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Description

Thy1/CD90 protein may play a role in cell-cell interactions, especially during synaptogenesis in the brain. It facilitates communication and connections between cells, shaping neural network architecture and functionality. Understanding how Thy1/CD90 participates in these interactions can provide insights into its role in brain function and connectivity. Thy1/CD90 Protein, Mouse (HEK293, His) is the recombinant mouse-derived Thy1/CD90 protein, expressed by HEK293 , with C-His labeled tag.

Background

The Thy1/CD90 protein emerges as a potential player in cell-cell or cell-ligand interactions, particularly during crucial events such as synaptogenesis in the brain. This implies a role for Thy1/CD90 in facilitating communication and connections between cells, contributing to the intricate processes involved in the formation and organization of synapses. The specific involvement of Thy1/CD90 in these interactions highlights its potential significance in shaping the architecture and functionality of neural networks. Further exploration into the precise mechanisms by which Thy1/CD90 participates in cell-cell or cell-ligand interactions during various neurodevelopmental events could provide valuable insights into its role in brain function and connectivity.

Biological Activity

Measured by its binding ability in a functional ELISA. When Recombinant Mouse CD90/Thy-1 is immobilized at 5.00 µg/mL (100 µL/well), Recombinant Mouse Galectin-1 binds with an ED50 of 0.203 μg/mL.

  • Measured by its binding ability in a functional ELISA. When Recombinant Mouse CD90/Thy-1 is immobilized at 5.00 µg/mL (100 µL/well), Recombinant Mouse Galectin-1 binds with an ED50 of 0.203 μg/mL.
Species

Mouse

Source

HEK293

Tag

C-His

Accession

P01831 (Q20-C131)

Gene ID
Molecular Construction
N-term
Thy1 (Q20-C131)
Accession # P01831
His
C-term
Protein Length

Full Length of Thy1

Synonyms
Thy-1 membrane glycoprotein; CDw90; Thy-1 antigen; CD90
AA Sequence

QKVTSLTACLVNQNLRLDCRHENNTKDNSIQHEFSLTREKRKHVLSGTLGIPEHTYRSRVTLSNQPYIKVLTLANFTTKDEGDYFCELQVSGANPMSSNKSISVYRDKLVKC

Predicted Molecular Mass
12.8 kDa
Molecular Weight

Approximately 19-26 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

Glycosylation
Yes
Purity
  • ≥ 95%, as determined by reducing SDS-PAGE.
Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation
References
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Thy1/CD90 Protein, Mouse (HEK293, His)

 

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Product Name:
Thy1/CD90 Protein, Mouse (HEK293, His)
Cat. No.:
HY-P74515
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