S-n-Butyrylthiocholine iodide
Based on 1 Customer Validation
S-n-Butyrylthiocholine iodide is a substrate of BChE. S-n-Butyrylthiocholine iodide commonly used for in vitro enzymatic experiments.
For research use only. We do not sell to patients.
- Purity: 99.74%
- CAS No.: 1866-16-6
- Formula: C9H20INOS
- Molecular Weight:317.23
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Storage:
-20°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Chemical Information
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CAS No. 1866-16-6
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Appearance Solid
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Molecular Weight 317.23
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Formula C9H20INOS
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Color White to off-white
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SMILES
CCCC(SCC[N+](C)(C)C)=O.[I-]
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
Solvent & Solubility
H2O : 125 mg/mL (394.04 mM; Need ultrasonic)
DMSO : 66.67 mg/mL (210.16 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (7.88 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (7.88 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
Purity & Documentation
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Data Sheet (268 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Handling Instructions (2659 KB)
References
[1]. Qu Z, et al. Determination of butyrylcholinesterase activity based on thiamine luminescence modulated by MnO2 nanosheets. Talanta. 2021 Mar 1;224:121831. [Content Brief]
[2]. Masson P, et al. High activity of human butyrylcholinesterase at low pH in the presence of excess butyrylthiocholine. Eur J Biochem. 2003 Jan;270(2):315-24. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO / H2O | 1 mM | 3.1523 mL | 15.7614 mL | 31.5229 mL | 78.8072 mL |
| 5 mM | 0.6305 mL | 3.1523 mL | 6.3046 mL | 15.7614 mL | |
| 10 mM | 0.3152 mL | 1.5761 mL | 3.1523 mL | 7.8807 mL | |
| 15 mM | 0.2102 mL | 1.0508 mL | 2.1015 mL | 5.2538 mL | |
| 20 mM | 0.1576 mL | 0.7881 mL | 1.5761 mL | 3.9404 mL | |
| 25 mM | 0.1261 mL | 0.6305 mL | 1.2609 mL | 3.1523 mL | |
| 30 mM | 0.1051 mL | 0.5254 mL | 1.0508 mL | 2.6269 mL | |
| 40 mM | 0.0788 mL | 0.3940 mL | 0.7881 mL | 1.9702 mL | |
| 50 mM | 0.0630 mL | 0.3152 mL | 0.6305 mL | 1.5761 mL | |
| 60 mM | 0.0525 mL | 0.2627 mL | 0.5254 mL | 1.3135 mL | |
| 80 mM | 0.0394 mL | 0.1970 mL | 0.3940 mL | 0.9851 mL | |
| 100 mM | 0.0315 mL | 0.1576 mL | 0.3152 mL | 0.7881 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
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