Scientific Reviews
Product Guides
Blogs
Topics
Targeted therapy! The Capetin Prize winning "Click Chemistry" can be used like this!Targeted therapy! The Nobel Prize winning "Click Chemistry" can be used like this!2025-02-06
404 Results for "
phosphate buffer
" in MedChemExpress (MCE) Product Catalog:
Products (404)
404 Results for "phosphate buffer" in MCE Product Catalog:
Cat. No.: HY-D3119
CAS No.: 2246946-49-4
Research Areas:
MitoAIE1 is a Fluorescent probe for mitochondrial viscosity detection. MitoAIE1 contains a pyridinium structural unit, which endows it with mitochondria-targeting specificity. The detection mechanism of this probe is based on aggregation-induced emission (AIE): in low-viscosity media such as PBS buffer, intramolecular rotation leads to non-radiative energy dissipation, resulting in only weak fluorescence; however, in high-viscosity environments such as the mitochondrial matrix or the vicinity of the inner mitochondrial membrane, such intramolecular motion is restricted, thereby significantly enhancing its fluorescent signal; in addition, this probe is not interfered by changes in microenvironment polarity and pH. Its emission wavelength is 625 nm, with absorption peaks at 325 nm and 450 nm; when transferred from low-viscosity PBS to high-viscosity 99% glycerol, its fluorescence intensity at 625 nm can be increased by 38-fold. It can be used to monitor changes in mitochondrial viscosity during processes such as Stauroporine (HY-15141)-induced apoptosis and starvation-induced mitophagy in live cells, and has good biocompatibility .
Cat. No.: HY-134096
CAS No.: 78323-98-5
Synonyms: DNS-M
Target:
Research Areas:
Dansyl-morpholine (DNS-M) is a Fluorescent probe for lipid droplet imaging, cancer cell discrimination, and real-time tracking of lipid droplet dynamics. As a solvatochromic probe with a donor-π-acceptor structure, it relies on hydrophobic interaction for its mechanism of action: its good lipophilicity, confirmed by an oil-water partition coefficient LogP = 2.35, allows it to rapidly penetrate cell membranes, and it specifically localizes to the hydrophobic core of lipid droplets; its fluorescence is strongly enhanced in the nonpolar environment of lipid droplets, while it emits very weak fluorescence in polar environments like PBS buffer, and it exhibits a bathochromic shift in emission wavelength with increasing solvent polarity. It has negligible cytotoxicity, with cell viability remaining over 95% after 24-hour incubation with 100 μM of the probe, and it possesses excellent photostability, retaining over 97% of initial fluorescence intensity after 60 continuous laser scans. For cell imaging applications, its excitation/emission wavelengths for lipid droplet labeling are Ex/Em = 405/480−540 nm, and in a simulative lipid environment O/W emulsion, it has an excitation wavelength of ~346 nm and emission wavelength of ~500 nm, giving a Stokes shift of 154 nm[1].
Gradient elution with sodium acetate buffer-ACN eluent on two ZIC-HILIC homemade columns separates 2-deoxyuridine in under 9 min .
In Vivo:2'-Deoxyuridine (34.42 ng/mL, gavage, 15 min) passes the blood-brain barrier (BBB) to enter the hippocampus of mice brain . 2'-Deoxyuridine (20 mg/kg, gavage, daily for 4 weeks) improves cognition and memory loss and attenuates the damage to the hippocampus in Aβ25-35-induced mice model .
-
-
Protocols
Targeted therapy! The Capetin Prize winning "Click Chemistry" can be used like this!Targeted therapy! The Nobel Prize winning "Click Chemistry" can be used like this!A suitable model is crucial in drug screening experiments. Organs can mimic the three-dimensional functional structure of internal organs, have similar spatial organization to corresponding organs, maintain some key characteristics, and reproduce some physiological functions. They are widely used for modeling and personalized drug screening physiological functions.2025-02-06
