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RuBP carboxylase

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By Targets:	Biochemical Assay Reagents (3) Endogenous Metabolite (1) Environmental Pollutants (1)
By Research Areas:	Metabolic Disease (2)

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Biochemical Assay Reagents

Natural
Products

Targets Recommended: Pyruvate Carboxylase (PC) Acetyl-CoA Carboxylase

Cat. No.	Product Name	Target	Research Areas	Chemical Structure

HY-164642

Ribulose 1,5-bisphosphate RuBP	Endogenous Metabolite	Metabolic Disease
Ribulose 1,5-bisphosphate (RUBP) is a vital photosynthetic intermediate and substrate. Ribulose 1,5-bisphosphate acts as both product and substrate for Thermococcus kodakarensis KOD1 R15Pi. Ribulose 1,5-bisphosphate tightly binds to inactive **RuBP carboxylase** sites in plant leaves.Ribulose 1,5-bisphosphate serves as the key substrate for CO₂ fixation in photosynthesis. Ribulose 1,5-bisphosphate supports carboxylation and regeneration processes in photosynthesis. Ribulose 1,5-bisphosphate determines the dynamic transition temperature of photosynthetic control. Ribulose 1,5-bisphosphate can be used for photosynthesis and enzyme mechanism research .

HY-W017448

Mepiquat chloride N,N-Dimethylpiperidinium chloride; PIX	Environmental Pollutants Biochemical Assay Reagents	Others
Mepiquat chloride (N,N-Dimethylpiperidinium chloride) is a systemic plant growth regulator. Mepiquat chloride reduces the activity of *RuBP* carboxylase. Mepiquat chloride decreases plant height, total length of vegetative and fruiting branches, and total leaf area of cotton. Mepiquat chloride reduces the net photosynthetic rate of cotton leaves. Mepiquat chloride promotes starch accumulation in cotton leaves without altering sucrose content .

HY-P2744

Ribulose bisphosphate carboxylase Ribulose 1,5-bisphosphate carboxylase	Biochemical Assay Reagents	Others
Ribulose bisphosphate carboxylase (Ribulose 1,5-bisphosphate carboxylase) catalyses the carboxylation (CO₂ addition) or oxygenation (O₂ addition) of d-ribulose-1,5-bisphosphate (RuBP) and the subsequent carbon–carbon cleavage to form two molecules of 3-phospho-d-glycerate (PGA) (with CO₂) or one molecule of PGA and one molecule of 2-phospho-glycolate (PG; with O₂) .

HY-E70942

D-Ribulose 1,5-Diphosphate Carboxylase, Spinach	Biochemical Assay Reagents	Metabolic Disease
D-Ribulose 1,5-Diphosphate Carboxylase, Spinach (EC 4.1.1.39) is an enzyme involved in the first step of carbon fixation, catalyzing the carboxylation of ribulose-1,5-diphosphate (also known as RuBP).

Cat. No.	Product Name	Type

HY-W017448

Mepiquat chloride N,N-Dimethylpiperidinium chloride; PIX	Biochemical Assay Reagents
Mepiquat chloride (N,N-Dimethylpiperidinium chloride) is a systemic plant growth regulator. Mepiquat chloride reduces the activity of *RuBP* carboxylase. Mepiquat chloride decreases plant height, total length of vegetative and fruiting branches, and total leaf area of cotton. Mepiquat chloride reduces the net photosynthetic rate of cotton leaves. Mepiquat chloride promotes starch accumulation in cotton leaves without altering sucrose content .

Mepiquat chloride

N,N-Dimethylpiperidinium chloride; PIX

Biochemical Assay Reagents

Mepiquat chloride (N,N-Dimethylpiperidinium chloride) is a systemic plant growth regulator. Mepiquat chloride reduces the activity of RuBP carboxylase. Mepiquat chloride decreases plant height, total length of vegetative and fruiting branches, and total leaf area of cotton. Mepiquat chloride reduces the net photosynthetic rate of cotton leaves. Mepiquat chloride promotes starch accumulation in cotton leaves without altering sucrose content .

Cat. No.	Product Name	Category	Target	Chemical Structure

HY-164642

Ribulose 1,5-bisphosphate RuBP	Structural Classification Natural Products Endogenous metabolite Source Classification	Endogenous Metabolite
Ribulose 1,5-bisphosphate (RUBP) is a vital photosynthetic intermediate and substrate. Ribulose 1,5-bisphosphate acts as both product and substrate for Thermococcus kodakarensis KOD1 R15Pi. Ribulose 1,5-bisphosphate tightly binds to inactive **RuBP carboxylase** sites in plant leaves.Ribulose 1,5-bisphosphate serves as the key substrate for CO₂ fixation in photosynthesis. Ribulose 1,5-bisphosphate supports carboxylation and regeneration processes in photosynthesis. Ribulose 1,5-bisphosphate determines the dynamic transition temperature of photosynthetic control. Ribulose 1,5-bisphosphate can be used for photosynthesis and enzyme mechanism research .

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BODIPY 493/503 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Feb 1;16(1):1241. [Abstract]

MedchemExpress Validation 01

Western blot analysis of extracts from THP-1(lane 2(20μg), Jurkat (lane 3(20μg) and NIH3T3(lane 4(20μg) using FOXO1A (HY-P80132) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature. Western blot analysis of extracts from THP-1(lane 2(20μg), Jurkat (lane 3(20μg) and NIH3T3(lane 4(20μg) using FOXO1A (HY-P80132) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature. Western blot analysis of extracts from THP-1(lane 2(20μg), Jurkat (lane 3(20μg) and NIH3T3(lane 4(20μg) using FOXO1A (HY-P80132) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

BODIPY 493/503 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Feb 1;16(1):1241. [Abstract]

MedchemExpress Validation 01

MedchemExpress Validation 03

Western blot analysis of extracts from THP-1(lane 2(20μg), Jurkat (lane 3(20μg) and NIH3T3(lane 4(20μg) using FOXO1A (HY-P80132) Rabbit mAb. Proteins were transferred

MedchemExpress Validation 04

MedchemExpress Validation

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RuBP carboxylase

Inhibitors & Agonists

Biochemical Assay Reagents

NaturalProducts

Natural
Products