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bio-orthogonal conjugation

" in MedChemExpress (MCE) Product Catalog:

標的別:	Biochemical Assay Reagents (1) Fluorescent Dye (2)
研究分野別:	Cancer (1) Inflammation/Immunology (1)
クリックケミストリー:	TCO (1) Labeling and Fluorescence Imaging (1) Tetrazine (1)

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蛍光色素

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製品番号	製品名	Target	研究分野	構造式

HY-114354

BODIPY FL alkyne BOD FL alkyne	Fluorescent Dye	Inflammation/Immunology
BODIPY (BOD) FL alkyne is an alkyne-containing BODIPY fluorophore derivative. BODIPY FL alkyne is a bioorthogonal labeling reagent with low toxicity and extremely low non-specific reactivity, and it is widely used in fluorescent bioimaging. BODIPY FL alkyne specifically labels azide groups on intracellular glycoconjugates mainly via strain-promoted azide-alkyne cycloaddition (SPAAC), or mediates site-specific conjugation with proteins such as IL-33, and supports positive cross-linking with other probes (e.g., DBCO-SCy5) for dual labeling. With the advantages of high specificity and low background interference, BODIPY FL alkyne can be used in the research of related diseases such as asthma, atopic dermatitis and inflammatory bowel disease .

HY-151759

Sulfo-Cy5-TCO	Fluorescent Dye	Cancer
Sulfo-Cy5-TCO is a water-soluble sulfonated Cy5 fluorescent reagent containing trans-cyclooctene (TCO). Sulfo-Cy5-TCO undergoes an inverse electron demand Diels‑Alder reaction with tetrazine-labeled fixed breast cancer cells to achieve cell labeling. Sulfo-Cy5-TCO is used for fluorescent labeling and imaging of biological samples such as proteins and cells .

HY-151686

Bz-(Me)Tz-NHS	Biochemical Assay Reagents	Others
Bz-(Me)Tz-NHS is a methyltetrazine carboxylic acid NHS ester that enables conjugation of the tetrazine moiety to the 5' end of oligonucleotides via amide coupling reaction, and it is used in oligonucleotide template-mediated fluorescent bioorthogonal cycloaddition reactions. Bz-(Me) Tz-NHS is applicable to click chemistry-related studies .

製品番号	製品名	Type

HY-114354

BODIPY FL alkyne BOD FL alkyne	蛍光色素
BODIPY (BOD) FL alkyne is an alkyne-containing BODIPY fluorophore derivative. BODIPY FL alkyne is a bioorthogonal labeling reagent with low toxicity and extremely low non-specific reactivity, and it is widely used in fluorescent bioimaging. BODIPY FL alkyne specifically labels azide groups on intracellular glycoconjugates mainly via strain-promoted azide-alkyne cycloaddition (SPAAC), or mediates site-specific conjugation with proteins such as IL-33, and supports positive cross-linking with other probes (e.g., DBCO-SCy5) for dual labeling. With the advantages of high specificity and low background interference, BODIPY FL alkyne can be used in the research of related diseases such as asthma, atopic dermatitis and inflammatory bowel disease .

BODIPY FL alkyne

BOD FL alkyne

蛍光色素

BODIPY (BOD) FL alkyne is an alkyne-containing BODIPY fluorophore derivative. BODIPY FL alkyne is a bioorthogonal labeling reagent with low toxicity and extremely low non-specific reactivity, and it is widely used in fluorescent bioimaging. BODIPY FL alkyne specifically labels azide groups on intracellular glycoconjugates mainly via strain-promoted azide-alkyne cycloaddition (SPAAC), or mediates site-specific conjugation with proteins such as IL-33, and supports positive cross-linking with other probes (e.g., DBCO-SCy5) for dual labeling. With the advantages of high specificity and low background interference, BODIPY FL alkyne can be used in the research of related diseases such as asthma, atopic dermatitis and inflammatory bowel disease .

HY-151759

Sulfo-Cy5-TCO	蛍光色素
Sulfo-Cy5-TCO is a water-soluble sulfonated Cy5 fluorescent reagent containing trans-cyclooctene (TCO). Sulfo-Cy5-TCO undergoes an inverse electron demand Diels‑Alder reaction with tetrazine-labeled fixed breast cancer cells to achieve cell labeling. Sulfo-Cy5-TCO is used for fluorescent labeling and imaging of biological samples such as proteins and cells .

製品番号	製品名		Classification

HY-151759

Sulfo-Cy5-TCO		Labeling and Fluorescence Imaging TCO
Sulfo-Cy5-TCO is a water-soluble sulfonated Cy5 fluorescent reagent containing trans-cyclooctene (TCO). Sulfo-Cy5-TCO undergoes an inverse electron demand Diels‑Alder reaction with tetrazine-labeled fixed breast cancer cells to achieve cell labeling. Sulfo-Cy5-TCO is used for fluorescent labeling and imaging of biological samples such as proteins and cells .

HY-151686

Bz-(Me)Tz-NHS		Tetrazine
Bz-(Me)Tz-NHS is a methyltetrazine carboxylic acid NHS ester that enables conjugation of the tetrazine moiety to the 5' end of oligonucleotides via amide coupling reaction, and it is used in oligonucleotide template-mediated fluorescent bioorthogonal cycloaddition reactions. Bz-(Me) Tz-NHS is applicable to click chemistry-related studies .

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BODIPY 493/503 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Feb 1;16(1):1241. [Abstract]

MedchemExpress Validation 01

Western blot analysis of extracts from THP-1(lane 2(20μg), Jurkat (lane 3(20μg) and NIH3T3(lane 4(20μg) using FOXO1A (HY-P80132) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature. Western blot analysis of extracts from THP-1(lane 2(20μg), Jurkat (lane 3(20μg) and NIH3T3(lane 4(20μg) using FOXO1A (HY-P80132) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature. Western blot analysis of extracts from THP-1(lane 2(20μg), Jurkat (lane 3(20μg) and NIH3T3(lane 4(20μg) using FOXO1A (HY-P80132) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

BODIPY 493/503 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Feb 1;16(1):1241. [Abstract]

MedchemExpress Validation 01

MedchemExpress Validation 03

Western blot analysis of extracts from THP-1(lane 2(20μg), Jurkat (lane 3(20μg) and NIH3T3(lane 4(20μg) using FOXO1A (HY-P80132) Rabbit mAb. Proteins were transferred

MedchemExpress Validation 04

MedchemExpress Validation

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