Bz-(Me)Tz-NHS
Based on 1 Customer Validation
Bz-(Me)Tz-NHS is a methyltetrazine carboxylic acid NHS ester that enables conjugation of the tetrazine moiety to the 5' end of oligonucleotides via amide coupling reaction, and it is used in oligonucleotide template-mediated fluorescent bioorthogonal cycloaddition reactions. Bz-(Me) Tz-NHS is applicable to click chemistry-related studies.
For research use only. We do not sell to patients.
- Purity: 95.02%
- CAS No.: 1454558-58-7
- Formula: C19H20N6O5
- Molecular Weight:412.40
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Storage:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Bz-(Me)Tz-NHS (100 mM; 5-fold excess) efficiently modifies amine-terminated DNA oligonucleotides via amide coupling in aqueous-bicarbonate buffer with minimal side reactions[1].
Bz-(Me)Tz-NHS generates quenched fluorogenic DNA probes that exhibit rapid template-dependent ligation and fluorescence activation upon reaction with cyclopropene-modified oligonucleotides[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 1454558-58-7
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Appearance Solid
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Molecular Weight 412.40
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Formula C19H20N6O5
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Color Purple to purplish red
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SMILES
O=C(CCCC(NCC1=CC=C(C2=NN=C(C)N=N2)C=C1)=O)ON3C(CCC3=O)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
DMSO : 25 mg/mL (60.62 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.4248 mL | 12.1242 mL | 24.2483 mL | 60.6208 mL |
| 5 mM | 0.4850 mL | 2.4248 mL | 4.8497 mL | 12.1242 mL | |
| 10 mM | 0.2425 mL | 1.2124 mL | 2.4248 mL | 6.0621 mL | |
| 15 mM | 0.1617 mL | 0.8083 mL | 1.6166 mL | 4.0414 mL | |
| 20 mM | 0.1212 mL | 0.6062 mL | 1.2124 mL | 3.0310 mL | |
| 25 mM | 0.0970 mL | 0.4850 mL | 0.9699 mL | 2.4248 mL | |
| 30 mM | 0.0808 mL | 0.4041 mL | 0.8083 mL | 2.0207 mL | |
| 40 mM | 0.0606 mL | 0.3031 mL | 0.6062 mL | 1.5155 mL | |
| 50 mM | 0.0485 mL | 0.2425 mL | 0.4850 mL | 1.2124 mL | |
| 60 mM | 0.0404 mL | 0.2021 mL | 0.4041 mL | 1.0103 mL |
Bz-(Me)Tz-NHS Related Classifications
HY-151686 Related Classifications
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- Bz-(Me)Tz-NHS
- 1454558-58-7
- Biochemical Assay Reagents
- aqueous-bicarbonate buffer
- tetrazine group
- methyl-tetrazine carboxylic acid NHS ester
- oligonucleotides
- fluorogenic template-driven bioorthogonal cycloaddition reactions
- mass spectrometry
- cyclopropene-modified oligonucleotides
- amine-terminated DNA oligonucleotides
- amide coupling
- HPLC
- Inhibitor
- inhibitor
- inhibit