Bz-(Me)Tz-NHS
Based on 1 Customer Validation
Bz-(Me)Tz-NHS is a methyltetrazine carboxylic acid NHS ester that enables conjugation of the tetrazine moiety to the 5' end of oligonucleotides via amide coupling reaction, and it is used in oligonucleotide template-mediated fluorescent bioorthogonal cycloaddition reactions. Bz-(Me) Tz-NHS is applicable to click chemistry-related studies.
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- Pureté : 95.02%
- CAS No.: 1454558-58-7
- Formule: C19H20N6O5
- Masse moléculaire:412.40
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Stockage:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
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Activité biologique
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Chemical Information
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Solvant et solubilité
- Protocole
- Pureté et documentation
- Références
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Help & FAQs
Activité biologique
Description
In Vitro
Bz-(Me)Tz-NHS (100 mM; 5-fold excess) efficiently modifies amine-terminated DNA oligonucleotides via amide coupling in aqueous-bicarbonate buffer with minimal side reactions[1].
Bz-(Me)Tz-NHS generates quenched fluorogenic DNA probes that exhibit rapid template-dependent ligation and fluorescence activation upon reaction with cyclopropene-modified oligonucleotides[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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CAS No. 1454558-58-7
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Appearance Solid
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Masse moléculaire 412.40
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Formule C19H20N6O5
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Color Purple to purplish red
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SMILES
O=C(CCCC(NCC1=CC=C(C2=NN=C(C)N=N2)C=C1)=O)ON3C(CCC3=O)=O
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvant et solubilité
In Vitro:
DMSO : 25 mg/mL (60.62 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Protocole
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EdU Incorporation Assay (Click Chemistry-Based DNA Synthesis Measurement)
The EdU incorporation assay measures DNA synthesis by adding the thymidine analog 5-ethynyl-2′-deoxyuridine to cells or tissues, where it is incorporated into newly synthesized DNA during S phase. Incorporated EdU is detected by copper-catalyzed azide-alkyne cycloaddition, in which a fluorescent azide covalently reacts with the ethynyl group on EdU, allowing S-phase cells to be detected by fluorescence microscopy, flow cytometry, or high-content imaging. EdU detection does not require DNA denaturation or anti-BrdU antibody access, which preserves sample structure and improves compatibility with immunostaining and multiparameter cytometry compared with BrdU-based detection. EdU can be cytotoxic in a cell-type- and exposure-dependent manner, so pulse duration, concentration, and continuous-labeling designs should be validated for each cell type.
Pureté et documentation
Références
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.4248 mL | 12.1242 mL | 24.2483 mL | 60.6208 mL |
| 5 mM | 0.4850 mL | 2.4248 mL | 4.8497 mL | 12.1242 mL | |
| 10 mM | 0.2425 mL | 1.2124 mL | 2.4248 mL | 6.0621 mL | |
| 15 mM | 0.1617 mL | 0.8083 mL | 1.6166 mL | 4.0414 mL | |
| 20 mM | 0.1212 mL | 0.6062 mL | 1.2124 mL | 3.0310 mL | |
| 25 mM | 0.0970 mL | 0.4850 mL | 0.9699 mL | 2.4248 mL | |
| 30 mM | 0.0808 mL | 0.4041 mL | 0.8083 mL | 2.0207 mL | |
| 40 mM | 0.0606 mL | 0.3031 mL | 0.6062 mL | 1.5155 mL | |
| 50 mM | 0.0485 mL | 0.2425 mL | 0.4850 mL | 1.2124 mL | |
| 60 mM | 0.0404 mL | 0.2021 mL | 0.4041 mL | 1.0103 mL |
Bz-(Me)Tz-NHS Related Classifications
HY-151686 Related Classifications
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Keywords
- Bz-(Me)Tz-NHS
- 1454558-58-7
- Biochemical Assay Reagents
- aqueous-bicarbonate buffer
- tetrazine group
- methyl-tetrazine carboxylic acid NHS ester
- oligonucleotides
- fluorogenic template-driven bioorthogonal cycloaddition reactions
- mass spectrometry
- cyclopropene-modified oligonucleotides
- amine-terminated DNA oligonucleotides
- amide coupling
- HPLC
- Inhibitor
- inhibitor
- inhibit