country

Change Language

EN - English
FR - Français
DE - Deutsch
ES - Español
JA - 日本語
KO - 한국어

Country or Region:

United States

Product Name	Size / Unit Price	Qty

Total: USD 0.00 Checkout

Cat. No.: HY-146817

Copy Link Print Information

Tubulin polymerization-IN-11

Tubulin polymerization-IN-11 is a potent tubulin polymerization inhibitor with an IC₅₀ value of 3.4 μM. Tubulin polymerization-IN-11 shows antiproliferative activity. Tubulin polymerization-IN-11 induces Apoptosis and cell cycle arrest at G2/M phase. Tubulin polymerization-IN-11 decreases the expression of cyclin B1, p-cdc2, and Bcl-2 protein levels and increases the expression of cleaved PARP.

For research use only. We do not sell to patients.

CAS No.: 2470063-59-1
Formula: C₂₂H₂₂N₄O₄
Molecular Weight:406.43

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

Data Sheet (274 KB)

Tubulin polymerization-IN-11 Chemical Structure

CAS No.: 2470063-59-1

Size

Price

Stock

Quantity

Amount: USD 0.00

* Please select Quantity before adding items.

Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- Cellular Effect
- In Vitro
Chemical Information
Purity & Documentation
References
Calculators
Help & FAQs
Classifications
Bioz

Biological Activity

Description

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	10.4 μM Compound: 7i	Antiproliferative activity against human A549 cells measured after 48 hrs by MTT assay Antiproliferative activity against human A549 cells measured after 48 hrs by MTT assay	[PMID: 32717486]
A549	IC₅₀	10.4 μM Compound: 6	Antiproliferative activity against human A549 cells assessed as inhibition of cell growth Antiproliferative activity against human A549 cells assessed as inhibition of cell growth	[PMID: 36089111]
HCT-116	IC₅₀	>50 μM Compound: 6	Antiproliferative activity against human HCT-116 cells assessed as inhibition of cell growth Antiproliferative activity against human HCT-116 cells assessed as inhibition of cell growth	[PMID: 36089111]
HEK293	IC₅₀	>100 μM Compound: 7i	Cytotoxicity against HEK293 cells measured after 48 hrs by MTT assay Cytotoxicity against HEK293 cells measured after 48 hrs by MTT assay	[PMID: 32717486]
HeLa	IC₅₀	0.012 μM Compound: 6	Antiproliferative activity against human HeLa cells assessed as inhibition of cell growth by MTT assay Antiproliferative activity against human HeLa cells assessed as inhibition of cell growth by MTT assay	[PMID: 36089111]
HeLa	IC₅₀	0.012 μM Compound: 7i	Antiproliferative activity against human HeLa cells measured after 48 hrs by MTT assay Antiproliferative activity against human HeLa cells measured after 48 hrs by MTT assay	[PMID: 32717486]
MCF7	IC₅₀	40.4 μM Compound: 7i	Antiproliferative activity against human MCF7 cells measured after 48 hrs by MTT assay Antiproliferative activity against human MCF7 cells measured after 48 hrs by MTT assay	[PMID: 32717486]
MCF7	IC₅₀	40.4 μM Compound: 6	Antiproliferative activity against human MCF7 cells assessed as inhibition of cell growth Antiproliferative activity against human MCF7 cells assessed as inhibition of cell growth	[PMID: 36089111]
T47D	IC₅₀	27.91 μM Compound: 7i	Antiproliferative activity against human T47D cells measured after 48 hrs by MTT assay Antiproliferative activity against human T47D cells measured after 48 hrs by MTT assay	[PMID: 32717486]
T98G	IC₅₀	>50 μM Compound: 6	Antiproliferative activity against human T98G cells assessed as inhibition of cell growth Antiproliferative activity against human T98G cells assessed as inhibition of cell growth	[PMID: 36089111]

In Vitro

Tubulin polymerization-IN-11 (compound 7i) (0-100 µM; 48 h) shows antiproliferative activity with IC₅₀s of 0.012, >100, 10.40, 40.40, 27.91 µM for HeLa, HEK-293, A549, MCF-7, T47D cells, respectively^[1].
Tubulin polymerization-IN-11 (12, 24, 48 nM; 24 h) induces apoptosis and cell cycle arrest at G2/M phase in a dose-dependent manner^[1].
Tubulin polymerization-IN-11 (12, 24, 48 nM; 24 h) decreases the expression of cyclin B1, p-cdc2, Bcl-2 protein levels and increases the expression of cleaved PARP in a dose-dependent manner^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay^[1]

Cell Line:

HeLa, HEK-293, A549, MCF-7, T47D cells
Concentration:

0-100 µM
Incubation Time:

48 h
Result:

Showed antiproliferative activity with IC₅₀s of 0.012, >100, 10.40, 40.40, 27.91 µM for HeLa, HEK-293, A549, MCF-7, T47D cells, respectively.

Cell Cycle Analysis^[1]

Cell Line:

HeLa cells
Concentration:

12, 24, 48 nM
Incubation Time:

24 h
Result:

Induced cell cycle arrest at G2/M phase with the percentage of cells was 13.90%, 26.00%, and 92.65% at 12, 24, 48 nM, respectively.

Western Blot Analysis^[1]

Cell Line:

HeLa cells
Concentration:

12, 24, 48 nM
Incubation Time:

24 h
Result:

Concentration-dependently decreased cyclin B1 and p-cdc2 protein levels.

Apoptosis Analysis^[1]

Cell Line:

HeLa cells
Concentration:

12, 24, 48 nM
Incubation Time:

24 h
Result:

Induced apoptosis with the total numbers of early and late apoptotic cells were 8.44%, 26.87% and 53.3% at 12, 24, and 48 nM, respectively.

Chemical Information

CAS No. 2470063-59-1
Molecular Weight 406.43
Formula C₂₂H₂₂N₄O₄
SMILES

COC1=CC=C(C=C1N)C2=CN3C(C=C2)=NN=C3C4=CC(OC)=C(C(OC)=C4)OC
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (274 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Yang F, et al. Synthesis, and biological evaluation of 3,6-diaryl-[1,2,4]triazolo[4,3-a]pyridine analogues as new potent tubulin polymerization inhibitors. Eur J Med Chem. 2020 Oct 15;204:112625.

Calculators

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Keywords

MOQ

Minimum order quantity

100 mg

Get Quote In-stock

Other size

Get Quote