Tyrosinase-IN-50
Based on 1 Customer Validation
Tyrosinase-IN-50 (Compound 14) is a Tyrosinase inhibitor (IC50 is 0.06 μM or 0.16 μM). Tyrosinase-IN-50 inhibits melanogenesis in multiple cell types. Tyrosinase-IN-50 can be used for the research of hyperpigmentation-related diseases and inflammatory diseases.
For research use only. We do not sell to patients.
- Purity : 99.50%
- CAS No.: 3081328-14-2
- Formula: C14H14N2O4S
- Molecular Weight:306.34
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Storage:
4°C, stored under nitrogen, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
In Vitro
Tyrosinase-IN-50 (5 min) exhibits an IC50 of 0.06 μM against tyrosinase in MNT-1 melanoma cells and an IC50 of 0.16 μM against tyrosinase in B16-F10 mouse skin melanoma cells[1].
Tyrosinase-IN-50 (1-10 μM; 6 days) inhibits melanogenesis in MNT-1 human melanoma cells in a concentration-dependent manner, with the melanin content at 10 μM being only 9.57% of that in the untreated group[1].
Tyrosinase-IN-50 (3.2-10000 μM) exhibits concentration-dependent DPPH radical scavenging activity, with the maximum scavenging rate reaching up to 49.45% at 10000 μM[1].
Tyrosinase-IN-50 (10 μM; 48 h) enhances the activity of the ARE/NRF2 reporter gene in HEK293T cells[1].
Tyrosinase-IN-50 (3-30 μM; 2 h) exerts a dose-dependent anti-inflammatory effect in LPS-induced RAW264.7 mouse macrophages[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:RAW264.7 mouse macrophage cell line
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Concentration:3-30 μM
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Incubation Time:2 h (pretreatment); 16 h (LPS treatment)
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Result:Downregulated the LPS-induced expression of IL-1α, IL-6, and TNF-α in a dose-dependent manner at concentrations of 3 μM, 10 μM, and 30 μM.
Chemical Information
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CAS No. 3081328-14-2
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Appearance Solid
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Molecular Weight 306.34
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Formula C14H14N2O4S
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SMILES
O=C(NC1=NC(=CS1)C=2C=CC(O)=CC2O)C3(C)COC3
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, stored under nitrogen, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen, away from moisture)
Solvent & Solubility
In Vitro:
DMSO : 100 mg/mL (326.43 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (8.16 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (8.16 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen, away from moisture)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.2643 mL | 16.3217 mL | 32.6435 mL | 81.6087 mL |
| 5 mM | 0.6529 mL | 3.2643 mL | 6.5287 mL | 16.3217 mL | |
| 10 mM | 0.3264 mL | 1.6322 mL | 3.2643 mL | 8.1609 mL | |
| 15 mM | 0.2176 mL | 1.0881 mL | 2.1762 mL | 5.4406 mL | |
| 20 mM | 0.1632 mL | 0.8161 mL | 1.6322 mL | 4.0804 mL | |
| 25 mM | 0.1306 mL | 0.6529 mL | 1.3057 mL | 3.2643 mL | |
| 30 mM | 0.1088 mL | 0.5441 mL | 1.0881 mL | 2.7203 mL | |
| 40 mM | 0.0816 mL | 0.4080 mL | 0.8161 mL | 2.0402 mL | |
| 50 mM | 0.0653 mL | 0.3264 mL | 0.6529 mL | 1.6322 mL | |
| 60 mM | 0.0544 mL | 0.2720 mL | 0.5441 mL | 1.3601 mL | |
| 80 mM | 0.0408 mL | 0.2040 mL | 0.4080 mL | 1.0201 mL | |
| 100 mM | 0.0326 mL | 0.1632 mL | 0.3264 mL | 0.8161 mL |
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