1. Cell Cycle/DNA Damage
  2. Deubiquitinase
  3. XL177A

XL177A 

Cat. No.: HY-138794
Handling Instructions

XL177A is a highly potent and selective irreversible USP7 inhibitor with an IC50 of 0.34 nM. XL177A elicits cancer cell killing through a p53-dependent mechanism.

For research use only. We do not sell to patients.

XL177A Chemical Structure

XL177A Chemical Structure

CAS No. : 2417089-74-6

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5 mg USD 480 In-stock
Estimated Time of Arrival: December 31
10 mg USD 800 In-stock
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25 mg USD 1650 In-stock
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50 mg USD 2600 In-stock
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100 mg USD 4000 In-stock
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Based on 1 publication(s) in Google Scholar

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Description

XL177A is a highly potent and selective irreversible USP7 inhibitor with an IC50 of 0.34 nM. XL177A elicits cancer cell killing through a p53-dependent mechanism[1].

In Vitro

XL177A is a potent USP7 inhibitor and p53 stabilizer in cyto. XL177A suppresses cancer cell growth predominantly through a p53-dependent mechanism. XL177A labels the catalytic cysteine, C223, of USP7 with exquisite selectivity for USP7 across the DUBome and human proteome[1].
XL177A (1 μM) induces complete G1 arrest in MCF7 cells after 24 hours[1].
Treatment of MCF7 cells, which express WT TP53, with XL177A (0.001- 10 μM) induces rapid degradation of HDM2 within 2 hours, followed by increases in p53 and downstream p21 protein levels[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis[1]

Cell Line: MCF7 cells
Concentration: 0.001, 0.01, 0.1, 1, 10 μM
Incubation Time: 18-24 hours
Result: The p53 and p21 protein levels remained high, but MDM2 protein levels matched DMSO control.
Molecular Weight

861.47

Formula

C₄₈H₅₇ClN₈O₅

CAS No.
SMILES

O=C(C(C=CC1=C2Cl)=CC1=NC3=C2CCCC3)NCCC[[email protected]@H](CC4=CC=CC=C4)C(N5CCC(CN6C=NC7=C(C=CC(NC(CCN8CCN(C)CC8)=O)=C7)C6=O)(O)CC5)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
References
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Keywords:

XL177ADeubiquitinaseDUBsUSP7cancerp53MCF7Inhibitorinhibitorinhibit

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XL177A
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HY-138794
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