ZBP1/RIP3/MLKL activator 1
Based on 1 Customer Validation
ZBP1/RIP3/MLKL activator 1 (compound 3a) is a synthetically derived quinoline compound. ZBP1/RIP3/MLKL Activator 1 induces DNA damage, enhances intracellular levels of reactive oxygen species (ROS), and triggers apoptosis via the caspase pathway. Furthermore, when apoptosis is inhibited, ZBP1/RIP3/MLKL Activator 1 promotes necroptotic cell death through the ZBP1-RIP3-MLKL pathway. ZBP1/RIP3/MLKL Activator 1 is utilized in oncological research, particularly in the selective targeting of cells with impaired apoptotic function.
For research use only. We do not sell to patients.
- Purity: 99.73%
- CAS No.: 3057611-79-4
- Formula: C26H28N6O2
- Molecular Weight:456.54
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Storage:
4°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Bioactive Compound Library Max
HY-L181
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Bioactive Compound Library
HY-L001
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Apoptosis Compound Library
HY-L003
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Immunology/Inflammation Compound Library
HY-L007
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MAPK Compound Library
HY-L010
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NF-κB Signaling Compound Library
HY-L014
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Anti-Cancer Compound Library
HY-L025
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Oxygen Sensing Compound Library
HY-L045
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Highly Selective Activators Library
HY-L159
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Cell Death Library
HY-L162
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Multi-Target Compound Library
HY-L176
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MCE Bioactive Compound Library
HY-L001V
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Protein Kinase Compound Library
HY-L196
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Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library
HY-L199
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High-Throughput Bioactive Compound Library
HY-L205
Biological Activity
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| HCT-116 | IC50 |
1.55 μM
Compound: 3a
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Cytotoxicity against human HCT-116 cells incubated for 24 hrs by MTT assay
Cytotoxicity against human HCT-116 cells incubated for 24 hrs by MTT assay
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[PMID: 38581731] |
| HEK-293T | IC50 |
14.2 μM
Compound: 3a
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Cytotoxicity against HEK293T cells incubated for 24 hrs by MTT assay
Cytotoxicity against HEK293T cells incubated for 24 hrs by MTT assay
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[PMID: 38581731] |
| HeLa | IC50 |
3.33 μM
Compound: 3a
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Cytotoxicity against human HeLa cells incubated for 24 hrs by MTT assay
Cytotoxicity against human HeLa cells incubated for 24 hrs by MTT assay
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[PMID: 38581731] |
| HT-29 | IC50 |
4 μM
Compound: 3a
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Cytotoxicity against human HT-29 cells incubated for 24 hrs by MTT assay
Cytotoxicity against human HT-29 cells incubated for 24 hrs by MTT assay
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[PMID: 38581731] |
| Huh-7 | IC50 |
6.9 μM
Compound: 3a
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Cytotoxicity against human Huh-7 cells incubated for 24 hrs by MTT assay
Cytotoxicity against human Huh-7 cells incubated for 24 hrs by MTT assay
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[PMID: 38581731] |
Chemical Information
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CAS No. 3057611-79-4
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Appearance Solid
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Molecular Weight 456.54
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Formula C26H28N6O2
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Color Yellow to orange
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SMILES
CN(C)CCCNC1=NC2=CC([N+]([O-])=O)=CC=C2N=C1NCC3=CC=C(C4=CC=CC=C4)C=C3
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
Solvent & Solubility
DMSO : 100 mg/mL (219.04 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 2.5 mg/mL (5.48 mM); Clear solution; Need ultrasonic
This protocol yields a clear solution of 2.5 mg/mL.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (271 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.1904 mL | 10.9519 mL | 21.9039 mL | 54.7597 mL |
| 5 mM | 0.4381 mL | 2.1904 mL | 4.3808 mL | 10.9519 mL | |
| 10 mM | 0.2190 mL | 1.0952 mL | 2.1904 mL | 5.4760 mL | |
| 15 mM | 0.1460 mL | 0.7301 mL | 1.4603 mL | 3.6506 mL | |
| 20 mM | 0.1095 mL | 0.5476 mL | 1.0952 mL | 2.7380 mL | |
| 25 mM | 0.0876 mL | 0.4381 mL | 0.8762 mL | 2.1904 mL | |
| 30 mM | 0.0730 mL | 0.3651 mL | 0.7301 mL | 1.8253 mL | |
| 40 mM | 0.0548 mL | 0.2738 mL | 0.5476 mL | 1.3690 mL | |
| 50 mM | 0.0438 mL | 0.2190 mL | 0.4381 mL | 1.0952 mL | |
| 60 mM | 0.0365 mL | 0.1825 mL | 0.3651 mL | 0.9127 mL | |
| 80 mM | 0.0274 mL | 0.1369 mL | 0.2738 mL | 0.6845 mL | |
| 100 mM | 0.0219 mL | 0.1095 mL | 0.2190 mL | 0.5476 mL |
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