Zidovudine O-β-D-glucuronide
Zidovudine O-β-D-glucuronide (3'-Azido-3'-deoxythymidine β-D-glucuronide) is the glucuronide conjugate and metabolite of Zidovudine (HY-17413), which can be used to detect UGT2B7 activity. As a substrate, Zidovudine O-β-D-glucuronide undergoes deconjugation via hydrolysis by immobilized β-glucuronidase to produce Zidovudine.
For research use only. We do not sell to patients.
- CAS No.: 117675-21-5
- Formula: C16H21N5O10
- Molecular Weight:443.37
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Zidovudine O-β-D-glucuronide (0.02-2.0 mM; 30 min) undergoes concentration-dependent hydrolysis by immobilized β-glucuronidase in an on-line reactor, with 98% conversion at 0.02 mM, while free β-glucuronidase achieves >98% conversion at all tested concentrations[2].
Zidovudine O-β-D-glucuronide (0.04 mM) undergoes 90% hydrolysis by immobilized β-glucuronidase at pH 5.0, and exposure of the reactor to pH 4.0 causes irreversible loss of activity, reducing subsequent hydrolysis at pH 5.0 to 12%[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 117675-21-5
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Molecular Weight 443.37
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Formula C16H21N5O10
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SMILES
O=C1N(C=C(C(N1)=O)C)[C@@H]2O[C@@H]([C@H](C2)N=[N+]=[N-])CO[C@@H]3O[C@@H]([C@H]([C@@H]([C@H]3O)O)O)C(O)=O
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Synonyms
3'-Azido-3'-deoxythymidine β-D-glucuronide
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
[1]. Walsky RL, et al. Optimized assays for human UDP-glucuronosyltransferase (UGT) activities: altered alamethicin concentration and utility to screen for UGT inhibitors. Drug Metab Dispos. 2012;40(5):1051-1065. [Content Brief]
[2]. Di Marco MP, et al. On-line deconjugation of glucuronides using an immobilized enzyme reactor based upon beta-glucuronidase. J Chromatogr B Biomed Sci Appl. 1998;715(2):379-386. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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