Zidovudine O-β-D-glucuronide sodium
Based on 1 Customer Validation
Zidovudine O-β-D-glucuronide (3'-Azido-3'-deoxythymidine β-D-glucuronide) sodium is the glucuronide conjugate and metabolite of Zidovudine (HY-17413), which can be used to detect UGT2B7 activity. As a substrate, Zidovudine O-β-D-glucuronide sodium undergoes deconjugation via hydrolysis by immobilized β-glucuronidase to produce Zidovudine.
For research use only. We do not sell to patients.
- Purity: 99.05%
- CAS No.: 133525-01-6
- Formula: C16H20N5NaO10
- Molecular Weight:465.35
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Storage:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Zidovudine O-β-D-glucuronide sodium (0.02-2.0 mM; 30 min) undergoes concentration-dependent hydrolysis by immobilized β-glucuronidase in an on-line reactor, with 98% conversion at 0.02 mM, while free β-glucuronidase achieves >98% conversion at all tested concentrations[2].
Zidovudine O-β-D-glucuronide sodium (0.04 mM) undergoes 90% hydrolysis by immobilized β-glucuronidase at pH 5.0, and exposure of the reactor to pH 4.0 causes irreversible loss of activity, reducing subsequent hydrolysis at pH 5.0 to 12%[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 133525-01-6
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Appearance Solid
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Molecular Weight 465.35
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Formula C16H20N5NaO10
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Color White to off-white
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SMILES
O=C(NC1=O)N(C=C1C)[C@](C[C@@H]2N=[N+]=[N-])([H])O[C@@H]2CO[C@@H]([C@@H]([C@H]3O)O)O[C@@H]([C@H]3O)C(O[Na])=O
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Synonyms
3'-Azido-3'-deoxythymidine β-D-glucuronide sodium
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
DMSO : 100 mg/mL (214.89 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (5.37 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (5.37 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (277 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Handling Instructions (2659 KB)
References
[1]. Walsky RL, et al. Optimized assays for human UDP-glucuronosyltransferase (UGT) activities: altered alamethicin concentration and utility to screen for UGT inhibitors. Drug Metab Dispos. 2012;40(5):1051-1065. [Content Brief]
[2]. Di Marco MP, et al. On-line deconjugation of glucuronides using an immobilized enzyme reactor based upon beta-glucuronidase. J Chromatogr B Biomed Sci Appl. 1998;715(2):379-386. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.1489 mL | 10.7446 mL | 21.4892 mL | 53.7230 mL |
| 5 mM | 0.4298 mL | 2.1489 mL | 4.2978 mL | 10.7446 mL | |
| 10 mM | 0.2149 mL | 1.0745 mL | 2.1489 mL | 5.3723 mL | |
| 15 mM | 0.1433 mL | 0.7163 mL | 1.4326 mL | 3.5815 mL | |
| 20 mM | 0.1074 mL | 0.5372 mL | 1.0745 mL | 2.6862 mL | |
| 25 mM | 0.0860 mL | 0.4298 mL | 0.8596 mL | 2.1489 mL | |
| 30 mM | 0.0716 mL | 0.3582 mL | 0.7163 mL | 1.7908 mL | |
| 40 mM | 0.0537 mL | 0.2686 mL | 0.5372 mL | 1.3431 mL | |
| 50 mM | 0.0430 mL | 0.2149 mL | 0.4298 mL | 1.0745 mL | |
| 60 mM | 0.0358 mL | 0.1791 mL | 0.3582 mL | 0.8954 mL | |
| 80 mM | 0.0269 mL | 0.1343 mL | 0.2686 mL | 0.6715 mL | |
| 100 mM | 0.0215 mL | 0.1074 mL | 0.2149 mL | 0.5372 mL |
Zidovudine O-β-D-glucuronide sodium Related Classifications
HY-137522 Related Classifications
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