Zn(II) Mesoporphyrin IX
Based on 1 Customer Validation
Zn (II) Mesoporphyrin IX (ZnMP) is a heme oxygenase inhibitor with photochemical substrate activity. Zn (II) Mesoporphyrin IX specifically inhibits the activity of bone marrow heme oxygenase. In vitro, Zn (II) Mesoporphyrin IX inhibits the growth of erythroid and myeloid progenitor cells in rabbit bone marrow, and blocks the rhG-CSF-induced mobilization of these progenitor cells into the peripheral blood, exhibiting toxicity to hematopoietic growth and progenitor cell production in rabbits. Zn (II) Mesoporphyrin IX undergoes irreversible photochemical decomposition conforming to first-order kinetic characteristics when irradiated with UV-B in 95% ethanol solution. Zn (II) Mesoporphyrin IX can be used in hematopoietic regulation research, but its photolability and toxic effects on the hematopoietic system require attention.
For research use only. We do not sell to patients.
- Purity: 95%
- CAS No.: 14354-67-7
- Formula: C34H36N4O4Zn
- Molecular Weight:630.05
-
Storage:
Store at room temperature 3 years.
In solvent -80°C, 2 years , -20°C, 1 year
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
-
Solvent & Solubility
- Purity & Documentation
- References
-
Help & FAQs
Biological Activity
Zn (II) Mesoporphyrin IX (5-20 μM; 10-12 days) exerts a dose-dependent inhibitory effect on the colony growth of BFU-E and CFU-GM in normal rabbit bone marrow cells, with significant inhibitory effects observed at concentrations of 5, 10, and 20 μM[1].
Zn (II) Mesoporphyrin IX (2.4 μM; exposed to UV-B irradiation for 0-7.5 min) undergoes UV-B-induced first-order photochemical decomposition in 95% ethanol with a rate constant of 17×10-3 min-1, and exhibits higher stability than free mesoporphyrin IX[2].
The apparent equilibrium constant for the formation of Zn (II) Mesoporphyrin IX from zinc sulfate and mesoporphyrin IX dimethyl ester in CTAB detergent decreases with decreasing temperature, with values of 6×10-3 M at 80°C, 2.5×10-3 M at 60°C, and 1×10-3 M at 20°C[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
-
Cell Line:Normal rabbit bone marrow cells
-
Concentration:5, 10, and 20 μM
-
Incubation Time:10-12 days
-
Result:Exhibited suppression of BFU-E and CFU-GM colony growth in a dose-dependent manner.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
-
Animal Model:New Zealand white (adult, male)[1]
-
Dosage:10 μmol/l·kg BW
-
Administration:i.v.; daily; 7 days
-
Result:Inhibited peripheral blood BFU-E mobilization by 76%, with only 24% of control BFU-E growth.
Inhibited peripheral blood CFU-GM mobilization by 70%, with only 30% of control CFU-GM growth.
Reduced bone marrow heme oxygenase activity to 0.765 nmol bilirubin/mg protein/h.
Chemical Information
-
CAS No. 14354-67-7
-
Appearance Solid
-
Molecular Weight 630.05
-
Formula C34H36N4O4Zn
-
Color Brown to reddish brown
-
SMILES
O=C(CCC1=C(C)C2=CC3=C(C)C(CC)=C4C=C5C(C)=C(CC)C6=[N]5[Zn+2]([N-]34)([N-]78)[N]2=C1C=C8C(CCC(O)=O)=C(C)C7=C6)O
-
Synonyms
ZnMP
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
Store at room temperature 3 years
In solvent -80°C 2 years -20°C 1 year
Solvent & Solubility
DMSO : 50 mg/mL (79.36 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 2.5 mg/mL (3.97 mM); Suspended solution; Need ultrasonic
This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
-
-
-
-
Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
-
%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
-
%+
-
+%Tween-80 + +
-
%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
-
Data Sheet (284 KB)
-
SDS (254 KB)
- English - EN (254 KB)
- Français - FR (254 KB)
- Deutsch - DE (254 KB)
- Norwegian - NO (254 KB)
- Español - ES (254 KB)
- Swedish - SV (254 KB)
- Italian - IT (254 KB)
- Korean - KR (254 KB)
- Portuguese - PT (254 KB)
-
Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.5872 mL | 7.9359 mL | 15.8718 mL | 39.6794 mL |
| 5 mM | 0.3174 mL | 1.5872 mL | 3.1744 mL | 7.9359 mL | |
| 10 mM | 0.1587 mL | 0.7936 mL | 1.5872 mL | 3.9679 mL | |
| 15 mM | 0.1058 mL | 0.5291 mL | 1.0581 mL | 2.6453 mL | |
| 20 mM | 0.0794 mL | 0.3968 mL | 0.7936 mL | 1.9840 mL | |
| 25 mM | 0.0635 mL | 0.3174 mL | 0.6349 mL | 1.5872 mL | |
| 30 mM | 0.0529 mL | 0.2645 mL | 0.5291 mL | 1.3226 mL | |
| 40 mM | 0.0397 mL | 0.1984 mL | 0.3968 mL | 0.9920 mL | |
| 50 mM | 0.0317 mL | 0.1587 mL | 0.3174 mL | 0.7936 mL | |
| 60 mM | 0.0265 mL | 0.1323 mL | 0.2645 mL | 0.6613 mL |
Zn(II) Mesoporphyrin IX Related Classifications
HY-W583212 Related Classifications
Powered by Bioz