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  3. Biochemical Assay Reagents
  4. 1,2-Bis(maleimido)ethane

1,2-Bis(maleimido)ethane  (Synonyms: N,N'-Ethylenedimaleimide)

Cat. No.: HY-W013538 Purity: 99.89%
COA
SDS
Handling Instructions Technical Support

1,2-Bis(maleimido)ethane (N,N'-Ethylenedimaleimide) is a protein cross-linking agent.

For research use only. We do not sell to patients.

1,2-Bis(maleimido)ethane

1,2-Bis(maleimido)ethane Chemical Structure

CAS No. : 5132-30-9

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 In-stock
Solution
10 mM * 1 mL in DMSO In-stock
Solid
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25 mg In-stock
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1,2-Bis(maleimido)ethane Related Antibodies

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Description

1,2-Bis(maleimido)ethane (N,N'-Ethylenedimaleimide) is a protein cross-linking agent.

Molecular Weight

220.18

Formula

C10H8N2O4
CAS No.
Appearance

Solid

Color

Off-white to light brown

SMILES

O=C(N1CCN2C(C=CC2=O)=O)C=CC1=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (454.17 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 4.5417 mL 22.7087 mL 45.4174 mL
5 mM 0.9083 mL 4.5417 mL 9.0835 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: 99.89%

SDS (393 KB)

COA (258 KB) HNMR (116 KB) RP-HPLC (87 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 4.5417 mL 22.7087 mL 45.4174 mL 113.5435 mL
5 mM 0.9083 mL 4.5417 mL 9.0835 mL 22.7087 mL
10 mM 0.4542 mL 2.2709 mL 4.5417 mL 11.3543 mL
15 mM 0.3028 mL 1.5139 mL 3.0278 mL 7.5696 mL
20 mM 0.2271 mL 1.1354 mL 2.2709 mL 5.6772 mL
25 mM 0.1817 mL 0.9083 mL 1.8167 mL 4.5417 mL
30 mM 0.1514 mL 0.7570 mL 1.5139 mL 3.7848 mL
40 mM 0.1135 mL 0.5677 mL 1.1354 mL 2.8386 mL
50 mM 0.0908 mL 0.4542 mL 0.9083 mL 2.2709 mL
60 mM 0.0757 mL 0.3785 mL 0.7570 mL 1.8924 mL
80 mM 0.0568 mL 0.2839 mL 0.5677 mL 1.4193 mL
100 mM 0.0454 mL 0.2271 mL 0.4542 mL 1.1354 mL
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1,2-Bis(maleimido)ethane Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

1,2-Bis(maleimido)ethane5132-30-9N,N'-EthylenedimaleimideBiochemical Assay ReagentsInhibitorinhibitorinhibit

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