DTME
Based on 1 Customer Validation
DTME (Dithio-bis-maleimidoethane) is a molecular crosslinker. The molecular structure of DTME contains two maleimide groups connected by an ethane disulfide bond, and this structure enables it to react specifically with molecules containing thiol groups (such as cysteine residues) to form stable covalent bonds. DTME is commonly used to investigate and characterize protein structure (especially oligomerization) or protein interactions.
For research use only. We do not sell to patients.
- Purity : 99.03%
- CAS No.: 71865-37-7
- Formula: C12H12N2O4S2
- Molecular Weight:312.36
-
Storage:Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
-
Solvent & Solubility
- Purity & Documentation
- References
-
Help & FAQs
-
HY-W013538
1,2-Bis(maleimido)ethaneProtein Cross-Linking Agent -
HY-W011957
1,4-Bis-maleimidobutaneBiochemical Reagent -
HY-119526
Bismaleimide -
HY-W110331A
Polyethylene glycol bis(3-aminopropyl) ether (Mn 1500)Biochemical Assay Reagents -
HY-W014139
Naphthalene-2,3-dicarboxylic acidMetal-organic Framework -
HY-W008680
4,4'-Bis(4-aminophenoxy)biphenylBiphenyl Analogue -
HY-W110356
Azobenzene-3,3'-dicarboxylic acidMetal-organic Framework -
HY-W011693
1,4-PhenylenebismaleimideBiochemical Assay Reagent -
HY-W013712
GI-530159TREK1/2 Channels Opener -
HY-W110584
Carboxybetaine methacrylateAmphoteric Ionic Compound -
HY-140208
2,2-Oxybis(ethylamine)PROTAC Linkers -
HY-W043748
2,5-Dioxopyrrolidin-1-yl acrylateLysine Residue Modifier
Biological Activity
Description
In Vitro
DTME (100 μM; 5-60 min) supports in vitro selection of DAR and DAB deoxyribozymes, yielding 49% and 33% Diels-Alder activity after 10 rounds and 18% and 13% after shortened 5-min incubation[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
-
CAS No. 71865-37-7
-
Appearance Solid
-
Molecular Weight 312.36
-
Formula C12H12N2O4S2
-
Color White to off-white
-
SMILES
O=C(C=C1)N(CCSSCCN2C(C=CC2=O)=O)C1=O
-
Synonyms
Dithio-bis-maleimidoethane
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
Powder -20°C 3 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
In Vitro:
DMSO : 50 mg/mL (160.07 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
-
-
-
-
Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
-
%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
-
%+
-
+%Tween-80 + +
-
%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
-
Data Sheet (274 KB)
-
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
-
Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.2014 mL | 16.0072 mL | 32.0143 mL | 80.0359 mL |
| 5 mM | 0.6403 mL | 3.2014 mL | 6.4029 mL | 16.0072 mL | |
| 10 mM | 0.3201 mL | 1.6007 mL | 3.2014 mL | 8.0036 mL | |
| 15 mM | 0.2134 mL | 1.0671 mL | 2.1343 mL | 5.3357 mL | |
| 20 mM | 0.1601 mL | 0.8004 mL | 1.6007 mL | 4.0018 mL | |
| 25 mM | 0.1281 mL | 0.6403 mL | 1.2806 mL | 3.2014 mL | |
| 30 mM | 0.1067 mL | 0.5336 mL | 1.0671 mL | 2.6679 mL | |
| 40 mM | 0.0800 mL | 0.4002 mL | 0.8004 mL | 2.0009 mL | |
| 50 mM | 0.0640 mL | 0.3201 mL | 0.6403 mL | 1.6007 mL | |
| 60 mM | 0.0534 mL | 0.2668 mL | 0.5336 mL | 1.3339 mL | |
| 80 mM | 0.0400 mL | 0.2001 mL | 0.4002 mL | 1.0004 mL | |
| 100 mM | 0.0320 mL | 0.1601 mL | 0.3201 mL | 0.8004 mL |
DTME Related Classifications
HY-W106411 Related Classifications
Powered by Bioz