3-O-Caffeoylquinic acid methyl ester
Based on 1 Customer Validation
3-O-Caffeoylquinic acid methyl ester is a monocaffeoylquinic acid derivative and phenolic acid component, which can be found in the whole plant of Pyrrosia calvata (Backer) Ching and the flower buds of Lonicera japonica Thunb.
For research use only. We do not sell to patients.
- Purity: 99.46%
- CAS No.: 123483-19-2
- Formula: C17H20O9
- Molecular Weight:368.34
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Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| B16 | IC50 |
>40 μM
Compound: 2
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Inhibition of alpha-MSH-stimulated melanogenesis in mouse B16 cells assessed as reduction in melanin formation
Inhibition of alpha-MSH-stimulated melanogenesis in mouse B16 cells assessed as reduction in melanin formation
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[PMID: 28619537] |
| BV-2 | IC50 |
12.11 μM
Compound: 11
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Anti-neuroinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced nitric oxide production after 10 mins by Griess assay relative to control
Anti-neuroinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced nitric oxide production after 10 mins by Griess assay relative to control
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[PMID: 23462643] |
3-O-Caffeoylquinic acid methyl ester (20-100 μg/mL; 48 h) exhibits no significant cytotoxicity against HepG 2 and HepG 2.2.15 cells at concentrations up to 100 μg/mL[2].
3-O-Caffeoylquinic acid methyl ester (100 μg/mL; 9 days) shows no significant anti-HBV activity against HepG 2.2.15 cells, and exerts no obvious inhibitory effects on HBsAg secretion, HBeAg secretion, or HBV DNA replication[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:HepG 2 cells, HepG 2.2.15 cells
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Concentration:20 μg/mL, 50 μg/mL, 100 μg/mL
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Incubation Time:48 h
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Result:Showed no significant cytotoxicity against HepG 2 and HepG 2.2.15 cells, with cell viability remaining close to control levels at 100 μg/mL.
Chemical Information
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CAS No. 123483-19-2
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Appearance Solid
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Molecular Weight 368.34
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Formula C17H20O9
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Color White to off-white
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SMILES
O=C([C@@]1(O)C[C@@H](OC(/C=C/C2=CC=C(O)C(O)=C2)=O)[C@H](O)[C@H](O)C1)OC
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
DMSO : 100 mg/mL (271.49 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (6.79 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (6.79 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (273 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Chen YJ, et al. Chemical Constituents of Pyrrosia calvata. Nat Prod Commun. 2015;10(7):1191-1193. [Content Brief]
[2]. Ge L, et al. Novel caffeoylquinic acid derivatives from Lonicera japonica Thunb. flower buds exert pronounced anti-HBV activities. RSC Adv. 2018 Oct 15;8(62):35374-35385. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.7149 mL | 13.5744 mL | 27.1488 mL | 67.8721 mL |
| 5 mM | 0.5430 mL | 2.7149 mL | 5.4298 mL | 13.5744 mL | |
| 10 mM | 0.2715 mL | 1.3574 mL | 2.7149 mL | 6.7872 mL | |
| 15 mM | 0.1810 mL | 0.9050 mL | 1.8099 mL | 4.5248 mL | |
| 20 mM | 0.1357 mL | 0.6787 mL | 1.3574 mL | 3.3936 mL | |
| 25 mM | 0.1086 mL | 0.5430 mL | 1.0860 mL | 2.7149 mL | |
| 30 mM | 0.0905 mL | 0.4525 mL | 0.9050 mL | 2.2624 mL | |
| 40 mM | 0.0679 mL | 0.3394 mL | 0.6787 mL | 1.6968 mL | |
| 50 mM | 0.0543 mL | 0.2715 mL | 0.5430 mL | 1.3574 mL | |
| 60 mM | 0.0452 mL | 0.2262 mL | 0.4525 mL | 1.1312 mL | |
| 80 mM | 0.0339 mL | 0.1697 mL | 0.3394 mL | 0.8484 mL | |
| 100 mM | 0.0271 mL | 0.1357 mL | 0.2715 mL | 0.6787 mL |
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