Cat. No.: HY-W040045

Callistephin chloride

(Synonyms: Pelargonidin-3-O-glucoside chloride)

1 Cited Publications

고객리뷰

Based on 1 publication(s) in Google Scholar

Callistephin (Pelargonidin 3-O-glucoside) chloride is an anthocyanin. Callistephin chloride regulates the expression of inflammatory (reducing iNOS/TNF-α/COX-2) and apoptosis-related proteins by inhibiting p38 phosphorylation, and enhances the protective effect of Isoflurane (HY-A0134) on microglial cell damage. Callistephin chloride significantly reduces ROS levels, eliminates DPPH free radicals, protects retinal pigment epithelial cells, and inhibits lipid peroxidation. Callistephin chloride can alleviate glutamate excitotoxicity, reduce neuronal apoptosis, and protect cerebellar granule neurons. Callistephin chloride can inhibit the proliferation and metastasis of breast cancer cells by inducing apoptosis.

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Purity: 98.31%
CAS No.: 18466-51-8
화학식: C₂₁H₂₁ClO₁₀
분자량:468.84

보관:

4°C, sealed storage, away from moisture and light

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Callistephin chloride Chemical Structure

CAS No.: 18466-51-8

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Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- 제품 설명
- IC₅₀ & Target
- In Vitro
- In Vivo
Chemical Information
Publications (1)
용액&용해도
- In Vitro
- In Vivo
순도&문서
References
Help & FAQs
Classifications
Bioz

Biological Activity

제품 설명

IC₅₀ & Target^[2]

COX-2

p38

iNOS

All COX Isoforms :

All NO Synthase Isoforms :

All p38 MAPK Isoforms :

All TNF Receptor Isoforms :

In Vitro

Callistephin chloride (5-100 μg/mL) significantly alters the morphology of red blood cells, increases the density of the membrane's hydrophilic regions, and has no obvious effect on the hydrophobic regions of the membrane^[1].
Callistephin chloride (5-50 μg/mL, 30 min) inhibits erythrocyte membrane lipid oxidation induced with UVC and AAPH radical with IC₅₀s of 17.7 and 5.8 μg/mL^[1].
Callistephin (0-400 μM μM, 24 h) chloride enhances the protective effects of Isoflurane by modulating engulfment and apoptosis in C8-B4 cells involved in the modulation of inducible nitric oxide synthase, cytochrome c oxidase subunit 2, tumor necrosis factor α and NF-κB^[2].
Callistephin (0.1-1 μg/mL, 30 min) chloride can effectively protect retinal pigment epithelium (RPE) cells by decreasing intracellular ROS and increasing mitochondrial redox activity in blue-light irradiated ARPE-19 cells^[3].
Callistephin (100 μM μM, 24 h) chloride significantly protects cerebellar granule neurons (CGNs) from the toxicity of glutamate but has no capacity to defend neurons from nitric oxide (NO)-induced apoptosis^[4].
Callistephin-rich extracts (0-200 μg/mL, 24-72 h) chloride inhibits the proliferation of MCF-7 cells, induces cell apoptosis, and exhibits DPPH radical scavenging activity and iron ion reduction ability^[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

RT-PCR^[2]

Cell Line:

C8-B4 cells treated with LPS (HY-D1056) and IFN-γ
Concentration:

100 μM
Incubation Time:

24 h
Result:

Reduced the expressions of iNOS, TNF-α and COX-2, but slightly increase NF-κB.

Western Blot Analysis^[2]

Cell Line:

C8-B4 cells treated with LPS (HY-D1056) and IFN-γ
Concentration:

100 μM
Incubation Time:

24 h
Result:

Significantly increased the expression of iNOS and COX-2.
Effectively inhibited p38 phosphorylation.
Maintained the level of Bcl-xL and reduced PARP cleavage.

Apoptosis Analysis^[2]

Cell Line:

cerebellar granule neurons (CGNs)
Concentration:

100 μM
Incubation Time:

24 h
Result:

Reduced CGN apoptosis to 20%.

In Vivo

Callistephin (3 mg/kg, i.p., single dose) chloride increases the expression of neuroD induced by Aβ in rats^[6].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Hepatocyte damage assay established in adult male SD rats^[4]
Dosage:

3 mg/kg
Administration:

Intraperitoneal injection (i.p.), single dose, 2 weeks after Aβ injection
Result:

Significantly increased the number of NeuroD1-positive cells in the Aβ group.

Chemical Information

CAS No. 18466-51-8
Appearance Solid
분자량 468.84
화학식 C₂₁H₂₁ClO₁₀
Color Brown to black
SMILES

OC1=CC(O)=CC2=C1C=C(O[C@@H]3O[C@H](CO)[C@@H](O)[C@H](O)[C@H]3O)C(C4=CC=C(O)C=C4)=[O+]2.[Cl-]
Synonyms

Pelargonidin-3-O-glucoside chloride
Structure Classification
- Flavonoids
- Anthocyans
- Phenols
- Polyphenols
Initial Source
선적

Room temperature in continental US; may vary elsewhere.
보관

4°C, sealed storage, away from moisture and light

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Publications (1)

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Food Chem

Flavonoid-mediated metabolic underpinning quality variation in red bud-sport pear mutants. [Abstract]

2025 May 31:489:144992. PMID: 40466530

용액&용해도

In Vitro:

DMSO : ≥ 100 mg/mL (213.29 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 50 mg/mL (106.65 mM; Need ultrasonic)

* "≥" means soluble, but saturation unknown.

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.1329 mL

10.6646 mL

21.3292 mL

5 mM

0.4266 mL

2.1329 mL

4.2658 mL

10 mM

0.2133 mL

1.0665 mL

2.1329 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

몰농도 계산기

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

농도 희석 계산기

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (5.33 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (5.33 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Working solution concentration: 0.22 mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact

MedChemExpress (MCE) .

순도&문서

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (288 KB)
SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Korean - KR (396 KB)
- Portuguese - PT (396 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Bonarska-Kujawa D, et, al. Interaction of selected anthocyanins with erythrocytes and liposome membranes. Cell Mol Biol Lett. 2012 Jun;17(2):289-308. [Content Brief]

[2]. Zhao L, et al. Callistephin enhances the protective effects of isoflurane on microglial injury through downregulation of inflammation and apoptosis. Mol Med Rep. 2019 Jul;20(1):802-812. [Content Brief]

[3]. Yacout SM, Gaillard ER. The Anthocyanins, Oenin and Callistephin, Protect RPE Cells Against Oxidative Stress. Photochem Photobiol. 2017 Mar;93(2):590-599. [Content Brief]

[4]. Winter AN, et al. Chemical basis for the disparate neuroprotective effects of the anthocyanins, callistephin and kuromanin, against nitrosative stress. Free Radic Biol Med. 2017 Feb;103:23-34. [Content Brief]

[5]. Eroglu Ozkan E, et al. Antiproliferative effects of Turkish pomegranate (Punica granatum L.) extracts on MCF-7 human breast cancer cell lines with focus on antioxidant potential and bioactive compounds analyzed by LC-MS/MS. J Food Biochem. 2021 Sep;45(9):e13904. [Content Brief]

[6]. Ghasemi Moravej F, et al. The Effect of Callistephin on Amyloid Beta-Induced Neurotoxicity in the Hippocampus of Male Rats[J]. Anatomical Sciences, 2016, 13(4): 215-220.

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
H₂O / DMSO	1 mM	2.1329 mL	10.6646 mL	21.3292 mL	53.3231 mL
	5 mM	0.4266 mL	2.1329 mL	4.2658 mL	10.6646 mL
	10 mM	0.2133 mL	1.0665 mL	2.1329 mL	5.3323 mL
	15 mM	0.1422 mL	0.7110 mL	1.4219 mL	3.5549 mL
	20 mM	0.1066 mL	0.5332 mL	1.0665 mL	2.6662 mL
	25 mM	0.0853 mL	0.4266 mL	0.8532 mL	2.1329 mL
	30 mM	0.0711 mL	0.3555 mL	0.7110 mL	1.7774 mL
	40 mM	0.0533 mL	0.2666 mL	0.5332 mL	1.3331 mL
	50 mM	0.0427 mL	0.2133 mL	0.4266 mL	1.0665 mL
	60 mM	0.0355 mL	0.1777 mL	0.3555 mL	0.8887 mL
	80 mM	0.0267 mL	0.1333 mL	0.2666 mL	0.6665 mL
	100 mM	0.0213 mL	0.1066 mL	0.2133 mL	0.5332 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Keywords

Callistephin chloride

1 Cited Publications

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Callistephin chloride

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Publications Citing Use of MedChemExpress (MCE) Callistephin chloride

All COX Isoforms

All NO Synthase Isoforms

All p38 MAPK Isoforms

All TNF Receptor Isoforms

Biological Activity

Chemical Information

Publications (1)

용액&용해도

순도&문서

References

Help & FAQs

Complete Stock Solution Preparation Table

Callistephin chloride Related Classifications

HY-W040045 Related Classifications