Callistephin chloride
Based on 1 publication(s) in Google Scholar
Callistephin (Pelargonidin 3-O-glucoside) chloride is an anthocyanin. Callistephin chloride regulates the expression of inflammatory (reducing iNOS/TNF-α/COX-2) and apoptosis-related proteins by inhibiting p38 phosphorylation, and enhances the protective effect of Isoflurane (HY-A0134) on microglial cell damage. Callistephin chloride significantly reduces ROS levels, eliminates DPPH free radicals, protects retinal pigment epithelial cells, and inhibits lipid peroxidation. Callistephin chloride can alleviate glutamate excitotoxicity, reduce neuronal apoptosis, and protect cerebellar granule neurons. Callistephin chloride can inhibit the proliferation and metastasis of breast cancer cells by inducing apoptosis.
For research use only. We do not sell to patients.
- Purity: 98.31%
- CAS No.: 18466-51-8
- Formula: C21H21ClO10
- Molecular Weight:468.84
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Storage:
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Callistephin chloride
More
Biological Activity
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COX-2 |
p38 |
iNOS |
Callistephin chloride (5-100 μg/mL) significantly alters the morphology of red blood cells, increases the density of the membrane's hydrophilic regions, and has no obvious effect on the hydrophobic regions of the membrane[1].
Callistephin chloride (5-50 μg/mL, 30 min) inhibits erythrocyte membrane lipid oxidation induced with UVC and AAPH radical with IC50s of 17.7 and 5.8 μg/mL[1].
Callistephin (0-400 μM μM, 24 h) chloride enhances the protective effects of Isoflurane by modulating engulfment and apoptosis in C8-B4 cells involved in the modulation of inducible nitric oxide synthase, cytochrome c oxidase subunit 2, tumor necrosis factor α and NF-κB[2].
Callistephin (0.1-1 μg/mL, 30 min) chloride can effectively protect retinal pigment epithelium (RPE) cells by decreasing intracellular ROS and increasing mitochondrial redox activity in blue-light irradiated ARPE-19 cells[3].
Callistephin (100 μM μM, 24 h) chloride significantly protects cerebellar granule neurons (CGNs) from the toxicity of glutamate but has no capacity to defend neurons from nitric oxide (NO)-induced apoptosis[4].
Callistephin-rich extracts (0-200 μg/mL, 24-72 h) chloride inhibits the proliferation of MCF-7 cells, induces cell apoptosis, and exhibits DPPH radical scavenging activity and iron ion reduction ability[5].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:C8-B4 cells treated with LPS (HY-D1056) and IFN-γ
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Concentration:100 μM
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Incubation Time:24 h
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Result:Reduced the expressions of iNOS, TNF-α and COX-2, but slightly increase NF-κB.
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Cell Line:C8-B4 cells treated with LPS (HY-D1056) and IFN-γ
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Concentration:100 μM
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Incubation Time:24 h
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Result:Significantly increased the expression of iNOS and COX-2.
Effectively inhibited p38 phosphorylation.
Maintained the level of Bcl-xL and reduced PARP cleavage.
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Cell Line:cerebellar granule neurons (CGNs)
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Concentration:100 μM
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Incubation Time:24 h
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Result:Reduced CGN apoptosis to 20%.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Hepatocyte damage assay established in adult male SD rats[4]
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Dosage:3 mg/kg
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Administration:Intraperitoneal injection (i.p.), single dose, 2 weeks after Aβ injection
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Result:Significantly increased the number of NeuroD1-positive cells in the Aβ group.
Chemical Information
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CAS No. 18466-51-8
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Appearance Solid
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Molecular Weight 468.84
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Formula C21H21ClO10
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Color Brown to black
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SMILES
OC1=CC(O)=CC2=C1C=C(O[C@@H]3O[C@H](CO)[C@@H](O)[C@H](O)[C@H]3O)C(C4=CC=C(O)C=C4)=[O+]2.[Cl-]
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Synonyms
Pelargonidin-3-O-glucoside chloride
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Publications (1)
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Journal Impact Factor
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Most Recent
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Food Chem
Flavonoid-mediated metabolic underpinning quality variation in red bud-sport pear mutants. [Abstract]2025 May 31:489:144992. PMID: 40466530
Solvent & Solubility
DMSO : ≥ 100 mg/mL (213.29 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : 50 mg/mL (106.65 mM; Need ultrasonic)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (5.33 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (5.33 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
Purity & Documentation
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Data Sheet (288 KB)
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SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Korean - KR (396 KB)
- Portuguese - PT (396 KB)
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Handling Instructions (2659 KB)
References
[1]. Bonarska-Kujawa D, et, al. Interaction of selected anthocyanins with erythrocytes and liposome membranes. Cell Mol Biol Lett. 2012 Jun;17(2):289-308. [Content Brief]
[2]. Zhao L, et al. Callistephin enhances the protective effects of isoflurane on microglial injury through downregulation of inflammation and apoptosis. Mol Med Rep. 2019 Jul;20(1):802-812. [Content Brief]
[3]. Yacout SM, Gaillard ER. The Anthocyanins, Oenin and Callistephin, Protect RPE Cells Against Oxidative Stress. Photochem Photobiol. 2017 Mar;93(2):590-599. [Content Brief]
[4]. Winter AN, et al. Chemical basis for the disparate neuroprotective effects of the anthocyanins, callistephin and kuromanin, against nitrosative stress. Free Radic Biol Med. 2017 Feb;103:23-34. [Content Brief]
[5]. Eroglu Ozkan E, et al. Antiproliferative effects of Turkish pomegranate (Punica granatum L.) extracts on MCF-7 human breast cancer cell lines with focus on antioxidant potential and bioactive compounds analyzed by LC-MS/MS. J Food Biochem. 2021 Sep;45(9):e13904. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O / DMSO | 1 mM | 2.1329 mL | 10.6646 mL | 21.3292 mL | 53.3231 mL |
| 5 mM | 0.4266 mL | 2.1329 mL | 4.2658 mL | 10.6646 mL | |
| 10 mM | 0.2133 mL | 1.0665 mL | 2.1329 mL | 5.3323 mL | |
| 15 mM | 0.1422 mL | 0.7110 mL | 1.4219 mL | 3.5549 mL | |
| 20 mM | 0.1066 mL | 0.5332 mL | 1.0665 mL | 2.6662 mL | |
| 25 mM | 0.0853 mL | 0.4266 mL | 0.8532 mL | 2.1329 mL | |
| 30 mM | 0.0711 mL | 0.3555 mL | 0.7110 mL | 1.7774 mL | |
| 40 mM | 0.0533 mL | 0.2666 mL | 0.5332 mL | 1.3331 mL | |
| 50 mM | 0.0427 mL | 0.2133 mL | 0.4266 mL | 1.0665 mL | |
| 60 mM | 0.0355 mL | 0.1777 mL | 0.3555 mL | 0.8887 mL | |
| 80 mM | 0.0267 mL | 0.1333 mL | 0.2666 mL | 0.6665 mL | |
| 100 mM | 0.0213 mL | 0.1066 mL | 0.2133 mL | 0.5332 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Callistephin chloride Related Classifications
HY-W040045 Related Classifications
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