2. Immunology/Inflammation NF-κB Metabolic Enzyme/Protease
  3. Reactive Oxygen Species (ROS)
  4. Isoflurane

Isoflurane is a volatile anaesthetic. Isoflurane diminishs the effect of ROS activity. Isoflurane suppresses respiration. Isoflurane enables rapid anesthesia induction and emergence. Isoflurane protects against noise-induced hearing loss and tissue damage in mice. Isoflurane protects against renal ischemia and reperfusion injury and modulates leukocyte infiltration.

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Isoflurane

Isoflurane Chemical Structure

CAS No. : 26675-46-7

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10 mM * 1 mL in DMSO
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Based on 17 publication(s) in Google Scholar

Isoflurane Related Antibodies

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Description

Isoflurane is a volatile anaesthetic. Isoflurane diminishs the effect of ROS activity. Isoflurane suppresses respiration. Isoflurane enables rapid anesthesia induction and emergence. Isoflurane protects against noise-induced hearing loss and tissue damage in mice. Isoflurane protects against renal ischemia and reperfusion injury and modulates leukocyte infiltration[1][2][3][4].

In Vitro

Isoflurane (0.04-2000 μM) specifically inhibits complex I-coupled respiration in permeabilized thoracic tissues of *Drosophila melanogaster*, with an IC50 of approximately 356 μM[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Isoflurane Related Antibodies
In Vivo

Anesthesia with Isoflurane (1.2-1.5 vol%; inhalation; 3 h per day for 3 consecutive days) protects mice from noise-induced hearing loss and tissue damage, thereby reducing the severity of hearing threshold shifts and improving their reversibility, while increasing hair cell survival rate[2].
Anesthesia with 1.2% Isoflurane (inhalation) significantly alleviates renal ischemia-reperfusion injury in mice by improving renal function, reducing necrosis, and regulating inflammation and leukocyte infiltration[3].
Isoflurane (2.8%-5%; inhalation; 2-50 min) enables rapid anesthesia induction and emergence (1-2 min) in healthy female C57BL/6J mice[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: BALB/c (4 weeks of age, normal Preyer’s reflex, normal hearing threshold)[2]
Dosage: 1.2 to 1.5 vol%
Administration: inhaled; 3 hours per day; 3 consecutive days
Result: Recorded a pre-exposure hearing threshold of 14.1 ± 4 dB HL, which increased to 44.1 ± 7 dB HL 1 day after noise exposure, 35.8 ± 4 dB HL 1 week after exposure, and 26.5 dB HL 1 month after exposure. Observed 90% survival rate of outer hair cells in the basal turn (80% of the total length from the apex), and 100% survival rate of inner hair cells in the basal turn.
Animal Model: C57BL/6 (male, 25-30g, renal ischemia-reperfusion injury model)[3]
Dosage: 1.2%
Administration: inhaled
Result: Measured plasma creatinine at 0.9 ± 0.1 mg/dL at 3 h and 1.3 ± 0.1 mg/dL at 24 h after ischemia, values significantly lower than pentobarbital group at both time points; Detected renal tubular necrosis grade at 0.8 ± 0.3 at 24 h after ischemia, significantly lower than pentobarbital group; Recorded renal cortical myeloperoxidase activity at 1.8 ± 0.4 OD/min per mg protein at 24 h after ischemia, significantly lower than pentobarbital group; Quantified neutrophil infiltration at 13.5 ± 7.3 neutrophils/mm² at 24 h after ischemia, significantly lower than pentobarbital group; Reduced ICAM-1 expression, macrophage infiltration, pro-inflammatory mRNA (TNF-α, IL-1β, KC, ICAM-1) upregulation, and NFκB nuclear translocation compared to pentobarbital group; Observed significantly less CD3+, CD8+, and NK1.1+ lymphocyte infiltration at 3 h after ischemia; Found CD4+ lymphocyte infiltration not statistically different between isoflurane and pentobarbital groups at 3 h after ischemia; Noted only NK1.1+ lymphocyte reduction persisted at 24 h after ischemia, with CD3+ and CD8+ lymphocyte counts not significantly different between isoflurane and pentobarbital groups at 24 h after ischemia
Clinical Trial
Molecular Weight

184.49

Formula

C3H2ClF5O
CAS No.
Appearance

Liquid (Density: 1.5 g/cm3)

Color

Colorless to light yellow

SMILES

FC(F)(F)C(Cl)OC(F)F
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Pure form -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (542.03 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 5.4203 mL 27.1017 mL 54.2035 mL
5 mM 1.0841 mL 5.4203 mL 10.8407 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Purity & Documentation

Purity: 99.27%

SDS (419 KB)

COA (244 KB) HNMR (235 KB) GC (288 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 5.4203 mL 27.1017 mL 54.2035 mL 135.5087 mL
5 mM 1.0841 mL 5.4203 mL 10.8407 mL 27.1017 mL
10 mM 0.5420 mL 2.7102 mL 5.4203 mL 13.5509 mL
15 mM 0.3614 mL 1.8068 mL 3.6136 mL 9.0339 mL
20 mM 0.2710 mL 1.3551 mL 2.7102 mL 6.7754 mL
25 mM 0.2168 mL 1.0841 mL 2.1681 mL 5.4203 mL
30 mM 0.1807 mL 0.9034 mL 1.8068 mL 4.5170 mL
40 mM 0.1355 mL 0.6775 mL 1.3551 mL 3.3877 mL
50 mM 0.1084 mL 0.5420 mL 1.0841 mL 2.7102 mL
60 mM 0.0903 mL 0.4517 mL 0.9034 mL 2.2585 mL
80 mM 0.0678 mL 0.3388 mL 0.6775 mL 1.6939 mL
100 mM 0.0542 mL 0.2710 mL 0.5420 mL 1.3551 mL
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Isoflurane Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Isoflurane26675-46-7Reactive Oxygen Species (ROS)miceanaestheticrenal ischemia and reperfusion injuryhearing lossInhibitorinhibitorinhibit

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