4'-Methoxychalcone

Name: 4'-Methoxychalcone
Brand: MedChemExpress
SKU: HY-128400
Rating: 4.5 (1 reviews)

Cat. No.: HY-128400 Purity: 99.44%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

4'-Methoxychalcone regulates adipocyte differentiation through PPARγ activation. 4'-Methoxychalcone modulates the expression and secretion of various adipokines in adipose tissue that are involved in insulin sensitivity.

For research use only. We do not sell to patients.

4'-Methoxychalcone Chemical Structure

CAS No. : 959-23-9

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25 mg	USD 50	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 90	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 140	In-stock	Estimated Time of Arrival: December 31
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Customer Review

Based on 1 publication(s) in Google Scholar

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PARP PARP1 PARP2 PARP3 PARP4 TNKS1/PARP5A TNKS2/PARP5B PARP7 PARP10 PARP14 PARP15 PARP12 PARP16

Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

PPARγ^[1]

In Vitro

4'-Methoxychalcone (5 μM; 8 days) markedly increases the mRNA expression of adipogenic genes during adipocyte differentiation, PPARγ, aP2, FAS, adiponectin and GluT 4^[1].
4'-Methoxychalcone (5 μM; 8 days) reduces the upregulated mRNA expression of IL-6, PAI-1, and MCP-1 by TNF-α in preadipocyte 3T3-L1 cells^[1].
4'-Methoxychalcone (5 μM; 0-8 days) causes an increase in PPARγ expression during differentiation, while C/EBPβ protein expression is relatively unaffected^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

RT-PCR^[1]

Cell Line:	Preadipocyte 3T3-L1 cells
Concentration:	5 μM
Incubation Time:	0 day; 2 days; 4 days; 6 days; 8 days
Result:	Increased the mRNA expression of all adipogenic genes, except GluT1 in adipocyte.

Western Blot Analysis^[1]

Cell Line:	Preadipocyte 3T3-L1 cells
Concentration:	5 μM
Incubation Time:	8 days
Result:	Increased PPARγ expression in adipocyte.

Molecular Weight

238.28

Formula

C₁₆H₁₄O₂

CAS No.

959-23-9

Appearance

Solid

Color

White to light yellow

SMILES

O=C(C1=CC=C(OC)C=C1)/C=C/C2=CC=CC=C2

Structure Classification

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (209.84 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	4.1967 mL	20.9837 mL	41.9674 mL
5 mM	0.8393 mL	4.1967 mL	8.3935 mL
10 mM	0.4197 mL	2.0984 mL	4.1967 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (10.49 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.44%

Select Batch:

Data Sheet (272 KB) SDS (251 KB)

COA (244 KB) HNMR (228 KB) LCMS (264 KB)

Handling Instructions (2659 KB)

References

[1]. Han Y, et al. Regulatory effects of 4-methoxychalcone on adipocyte differentiation through PPARγ activation and reverse effect on TNF-α in 3T3-L1 cells. Food Chem Toxicol. 2017 Aug;106(Pt A):17-24. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	4.1967 mL	20.9837 mL	41.9674 mL	104.9186 mL
	5 mM	0.8393 mL	4.1967 mL	8.3935 mL	20.9837 mL
	10 mM	0.4197 mL	2.0984 mL	4.1967 mL	10.4919 mL
	15 mM	0.2798 mL	1.3989 mL	2.7978 mL	6.9946 mL
	20 mM	0.2098 mL	1.0492 mL	2.0984 mL	5.2459 mL
	25 mM	0.1679 mL	0.8393 mL	1.6787 mL	4.1967 mL
	30 mM	0.1399 mL	0.6995 mL	1.3989 mL	3.4973 mL
	40 mM	0.1049 mL	0.5246 mL	1.0492 mL	2.6230 mL
	50 mM	0.0839 mL	0.4197 mL	0.8393 mL	2.0984 mL
	60 mM	0.0699 mL	0.3497 mL	0.6995 mL	1.7486 mL
	80 mM	0.0525 mL	0.2623 mL	0.5246 mL	1.3115 mL
	100 mM	0.0420 mL	0.2098 mL	0.4197 mL	1.0492 mL