4-Methoxyphenyl isothiocyanate (Synonyms: 4-Methylphenyl ITC)

Cat. No.: HY-W540232 Purity: 99.59%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

4-Methoxyphenyl isothiocyanate (4-Methylphenyl ITC) is an antioxidant, with a IC₅₀ value of 1.25 mM for scavenging DPPH radicals. ORAC testing indicates its antioxidant capacity as 11.7 mM TE (indicating that the antioxidant efficacy of this compound is equivalent to 11.7 mmol of Trolox (HY-101445, a standard antioxidant) under the same conditions), and it extends the oxidation process by approximately 9180 seconds in the Briggs–Rauscher reaction. Additionally, 4-Methoxyphenyl isothiocyanate exhibits moderate cholinesterase inhibitory activity, with an inhibition rate of 30.4% against acetylcholinesterase (AChE) and 17.9% against butyrylcholinesterase (BChE). 4-Methoxyphenyl isothiocyanate holds potential for research in the fields of antioxidation and neurological disorders.

For research use only. We do not sell to patients.

4-Methoxyphenyl isothiocyanate Chemical Structure

CAS No. : 2284-20-0

Size	Stock	Quantity
Solid or liquid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid or liquid
1 g	In-stock	Estimated Time of Arrival: December 31
5 g	In-stock	Estimated Time of Arrival: December 31
10 g	Get quote
50 g	Get quote

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Description

Molecular Weight

165.21

Formula

C₈H₇NOS

CAS No.

2284-20-0

Appearance

Solid-Liquid Mixture

Color

Colorless to light yellow

SMILES

COC1=CC=C(N=C=S)C=C1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

Solvent & Solubility

In Vitro:

DMSO : 200 mg/mL (1210.58 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	6.0529 mL	30.2645 mL	60.5290 mL
5 mM	1.2106 mL	6.0529 mL	12.1058 mL
10 mM	0.6053 mL	3.0265 mL	6.0529 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 5 mg/mL (30.26 mM); Clear solution

This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.59%

Select Batch:

Data Sheet (270 KB) SDS (641 KB)

COA (246 KB) HNMR (128 KB) LCMS (327 KB) GC (179 KB)

Handling Instructions (2659 KB)

References

[1]. Burčul F, et al. Isothiocyanates: cholinesterase inhibiting, antioxidant, and anti-inflammatory activity. J Enzyme Inhib Med Chem. 2018 Dec;33(1):577-582. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	6.0529 mL	30.2645 mL	60.5290 mL	151.3226 mL
	5 mM	1.2106 mL	6.0529 mL	12.1058 mL	30.2645 mL
	10 mM	0.6053 mL	3.0265 mL	6.0529 mL	15.1323 mL
	15 mM	0.4035 mL	2.0176 mL	4.0353 mL	10.0882 mL
	20 mM	0.3026 mL	1.5132 mL	3.0265 mL	7.5661 mL
	25 mM	0.2421 mL	1.2106 mL	2.4212 mL	6.0529 mL
	30 mM	0.2018 mL	1.0088 mL	2.0176 mL	5.0441 mL
	40 mM	0.1513 mL	0.7566 mL	1.5132 mL	3.7831 mL
	50 mM	0.1211 mL	0.6053 mL	1.2106 mL	3.0265 mL
	60 mM	0.1009 mL	0.5044 mL	1.0088 mL	2.5220 mL
	80 mM	0.0757 mL	0.3783 mL	0.7566 mL	1.8915 mL
	100 mM	0.0605 mL	0.3026 mL	0.6053 mL	1.5132 mL

4-Methoxyphenyl isothiocyanate Related Classifications

Neurological Disease

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

4-Methoxyphenyl isothiocyanate2284-20-04-Methylphenyl ITCCholinesterase (ChE)Inhibitorinhibitorinhibit

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4-Methoxyphenyl isothiocyanate (Synonyms: 4-Methylphenyl ITC)

4-Methoxyphenyl isothiocyanate Related Antibodies

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All Cholinesterase (ChE) Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

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